PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS09905 [old locus tag: SAUSA300_1810 ]
• pan locus tag^?: SAUPAN004796000
• symbol: SAUSA300_RS09905
• pan gene symbol^?: tnp3
• synonym:
• product: ISL3 family transposase IS1181

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS09905 [old locus tag: SAUSA300_1810 ]
• symbol: SAUSA300_RS09905
• product: ISL3 family transposase IS1181
• replicon: chromosome
• strand: +
• coordinates: 1993637..1994956
• length: 1320
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (1993637..1994956) NCBI
• BioCyc: SAUSA300_RS09905 BioCyc
• MicrobesOnline: see SAUSA300_1810

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1021
  1081
  1141
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  1261
  
  ATGTGTAATGATACCTTAGAATTACTAAGAATAAAAGATGAAAATATAAAATATATAAAC
  CAAGAAATTGACGTCATTATCAAAGGAAAAAAAGCAACAGTGGTTAATGCTGTACTAACG
  TATAAGCCTTCGGCCTGTTATTGTTGTGGAGTTAAAAATGAAGGACAAATTCATAAACAT
  GGTAAGCGTGTTTCTCGTATTACTTTACTTAAAACTCAAGGGTATAACACATACCTCAAC
  TTAGCTAAACAACGTTTTAAATGCTTAGAATGCAATGGCACTTTTACTGCTAAAACGTCA
  ATTGTTGATGAGTCGTGTTTTATCTCAAGATGTGTTACTCAAAAAGTTATAGAAGAAGCT
  ACTAAAGTTAAAACAGAGATTGATACTGCAGAAGATAACTGTATCTCTCCATCTACTGTA
  AGTCGTATTAGAACTAAAGCGGCTAATTCATTACGAATTAAACCCTTTAATTGTTTGCCA
  GAACACATCGCTATGGATGAATTTAAAAGCGTTAAAAATGTAACTGGATCAATGAGTTTC
  ATTTTTATAGATAATGATACTCATGATGTTATAGATATTTTAGAAAATAGAACTACAAGA
  TTCTTGCGTGCCTATTTCGAGCGATTCGATTTAAAAAATCGACAACAAGTTAAGACGGTT
  ACTATTGACATGTATGAACCCTATGTCCGATTATTTCGCGACCTATTTCCTAATGCAGCT
  ATTATTTTTGACAGATTCCATATCGTTCAACATTTAAATAGAGAACTTAATAAGTATCGT
  GTACAAGTTATGAATGAATACCGTAATAAAAAAGGACCTGATTATACAATTTTTAAGAAT
  AACTGGAAAGTCCTATTGATGGATACTAGTAAAACCATATTTAGTAAATACAGATGGAAT
  AAATCTTTTAAGGCTTATAAACGCTCATCTGACATTGTAGAATTCATGCTTTCAAAAGAC
  GATATACTACGACACTCCTACGAACTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGT
  AATTGGCCTAAATTTATTAATCGTTTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGT
  GTATGGAAAGCGGTTAAATATTATAGAAAACATCAAAGGATGTTAAGAAATACAATTTAT
  TACCCAGCATTTAATAATGGTGCTATAGAAGGAATTAATAATAAGATAAAATTAATCAAG
  CGAATTTCTTTTGGTTACAGAAATTTCAACAACTTTAAAGCACGTATAATGATGATTTTC
  AGCTTGTACAAAGGAGAAAAAAAGAAGACAACCAAGCCCAATAATGGACTGGCCGCCTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS09905 [old locus tag: SAUSA300_1810 ]
• symbol: SAUSA300_RS09905
• description: ISL3 family transposase IS1181
• length: 439
• theoretical pI: 10.3811
• theoretical MW: 51524.6
• GRAVY: -0.489066

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Detoxification S-(hydroxymethyl)glutathione synthase (TIGR02820; EC 4.4.1.22; HMM-score: 16.5)
  Metabolism Central intermediary metabolism One-carbon metabolism S-(hydroxymethyl)glutathione synthase (TIGR02820; EC 4.4.1.22; HMM-score: 16.5)
• TheSEED: see SAUSA300_1810
• PFAM:
  RNase_H (CL0219) DDE_Tnp_ISL3; Transposase (PF01610; HMM-score: 172.2)
  and 4 more
  Zn_Beta_Ribbon (CL0167) zf-ISL3; zinc-finger of transposase IS204/IS1001/IS1096/IS1165 (PF14690; HMM-score: 42.4)
  HTH (CL0123) HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 20.6)
  no clan defined DUF3795; Protein of unknown function (DUF3795) (PF12675; HMM-score: 10.3)
  DUF4163; Domain of unknown function (DUF4163) (PF13739; HMM-score: 9.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.179974
  • TAT(Tat/SPI): 0.017784
  • LIPO(Sec/SPII): 0.012804
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 487723914 NCBI
• RefSeq: WP_001808146 NCBI
• UniProt: see SAUSA300_1810

⊟Protein sequence

• MCNDTLELLRIKDENIKYINQEIDVIIKGKKATVVNAVLTYKPSACYCCGVKNEGQIHKHGKRVSRITLLKTQGYNTYLNLAKQRFKCLECNGTFTAKTSIVDESCFISRCVTQKVIEEATKVKTEIDTAEDNCISPSTVSRIRTKAANSLRIKPFNCLPEHIAMDEFKSVKNVTGSMSFIFIDNDTHDVIDILENRTTRFLRAYFERFDLKNRQQVKTVTIDMYEPYVRLFRDLFPNAAIIFDRFHIVQHLNRELNKYRVQVMNEYRNKKGPDYTIFKNNWKVLLMDTSKTIFSKYRWNKSFKAYKRSSDIVEFMLSKDDILRHSYELVQGLRKDLRLCNWPKFINRLNSVSKKSVSKGVWKAVKYYRKHQRMLRNTIYYPAFNNGAIEGINNKIKLIKRISFGYRNFNNFKARIMMIFSLYKGEKKKTTKPNNGLAA

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications