PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS10090 [old locus tag: SAUSA300_1847 ]
• pan locus tag^?: SAUPAN004847000
• symbol: SAUSA300_RS10090
• pan gene symbol^?: —
• synonym:
• product: multidrug ABC transporter ATP-binding protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS10090 [old locus tag: SAUSA300_1847 ]
• symbol: SAUSA300_RS10090
• product: multidrug ABC transporter ATP-binding protein
• replicon: chromosome
• strand: -
• coordinates: 2008366..2010102
• length: 1737
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (2008366..2010102) NCBI
• BioCyc: SAUSA300_RS10090 BioCyc
• MicrobesOnline: see SAUSA300_1847

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGATTAAACGATATTTGCAATTTGTTAAGCCATACAAATATCGTATTTTTGCAACGATT
  ATTGTTGGGATAATTAAGTTTGGTATACCAATGCTTATACCACTATTAATTAAATATGCA
  ATAGATGGCGTGATTAATAACCACGCACTAACGACTGATGAAAAAGTTCATCATTTAACT
  ATTGCCATTGGTATCGCATTATTTATTTTTGTAATAGTGAGACCACCAATTGAATTTATA
  CGTCAATATTTGGCGCAATGGACAAGTAATAAAATATTGTATGATATACGTAAAAAGTTA
  TACAACCATTTACAAGCTTTAAGTGCGAGATTTTATGCTAATAATCAAGTAGGTCAAGTA
  ATATCTAGAGTGATTAATGATGTTGAACAAACAAAAGATTTCATTTTAACCGGGTTAATG
  AATATTTGGTTAGATTGTATAACAATTATTATTGCACTATCCATAATGTTCTTTTTAGAT
  GTGAAATTGACTTTAGCAGCACTGTTTATCTTCCCATTTTACATTTTAACGGTGTACGTT
  TTCTTTGGAAGATTAAGAAAATTGACACGTGAAAGATCTCAAGCATTAGCTGAGGTTCAA
  GGATTCTTGCATGAACGTGTTCAAGGTATTTCAGTCGTTAAAAGTTTTGCGATTGAAGAC
  AATGAAGCGAAAAACTTTGATAAAAAGAATACTAATTTCCTAACACGTGCGTTGAAACAT
  ACAAGATGGAATGCCTATTCCTTTGCCGCAATTAATACAGTTACAGATATTGGACCAATT
  ATTGTCATCGGTGTTGGTGCATATCTTGCTATTTCTGGATCAATCACAGTAGGTACACTT
  GCAGCATTTGTTGGATACTTAGAGTTATTGTTCGGGCCTTTACGTCGTTTAGTCGCATCA
  TTTACAACTTTAACGCAAAGTTTTGCTTCAATGGACCGTGTATTCCAATTAATTGATGAA
  GATTATGACATCAAAAATGGTGTTGGTGCTCAACCTATTGAAATTAAACAAGGTCGTATT
  GATATTGATCATGTTAGTTTTCAATATAACGATAACGAAGCTCCAATTTTAAAAGATATT
  AATTTGAGTATTGAAAAAGGAGAAACAGTTGCTTTCGTAGGTATGAGTGGTGGTGGTAAA
  TCAACATTAATTAACTTAATACCGAGATTTTACGATGTAACTTCTGGGCAAATTTTAATA
  GATGGTCACAACATTAAAGATTTTTTAACGGGAAGTTTAAGAAATCAAATAGGATTGGTG
  CAACAGGATAATATTTTATTCTCTGATACAGTTAAGGAAAATATTTTACTTGGTCGTCCA
  ACAGCAACAGATGAAGAAGTAGTTGAAGCGGCGAAAATGGCTAATGCACATGACTTTATT
  ATGAACTTGCCACAGGGATATGACACTGAAGTAGGTGAACGAGGTGTTAAATTATCAGGT
  GGTCAAAAACAAAGATTATCGATTGCTAGAATATTTTTAAATAATCCGCCAATTCTTATC
  TTGGATGAAGCAACAAGTGCACTTGATTTAGAAAGTGAATCCATTATTCAAGAAGCATTA
  GATGTGTTGAGTAAAGATCGAACGACACTTATCGTAGCGCATCGCTTGTCCACTATTACA
  CATGCTGACAAAATTGTCGTAATTGAAAATGGACATATTGTTGAAACAGGTACGCATCGT
  GAATTGATTGCAAAACAAGGTGCTTACGAGCATTTATATAGCATTCAAAACTTATAA
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  1737
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS10090 [old locus tag: SAUSA300_1847 ]
• symbol: SAUSA300_RS10090
• description: multidrug ABC transporter ATP-binding protein
• length: 578
• theoretical pI: 7.25358
• theoretical MW: 64862.8
• GRAVY: 0.196194

⊟Function

• reaction:
  EC 3.6.3.-?  ExPASy
• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides lipid A export permease/ATP-binding protein MsbA (TIGR02203; HMM-score: 517.4)
  Metabolism Transport and binding proteins Other lipid A export permease/ATP-binding protein MsbA (TIGR02203; HMM-score: 517.4)
  ABC transporter, permease/ATP-binding protein (TIGR02204; HMM-score: 487.5)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR01846; HMM-score: 450.1)
  Metabolism Transport and binding proteins Other antigen peptide transporter 2 (TIGR00958; HMM-score: 427.5)
  and 73 more
  Cellular processes Cellular processes Biosynthesis of natural products NHLM bacteriocin system ABC transporter, peptidase/ATP-binding protein (TIGR03796; HMM-score: 357.4)
  Metabolism Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system ABC transporter, peptidase/ATP-binding protein (TIGR03796; HMM-score: 357.4)
  Cellular processes Cellular processes Pathogenesis type I secretion system ATPase (TIGR03375; HMM-score: 355.7)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR03375; HMM-score: 355.7)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking ABC-type bacteriocin transporter (TIGR01193; HMM-score: 341)
  Genetic information processing Protein fate Protein modification and repair ABC-type bacteriocin transporter (TIGR01193; HMM-score: 341)
  Metabolism Transport and binding proteins Other ABC-type bacteriocin transporter (TIGR01193; HMM-score: 341)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids glucan exporter ATP-binding protein (TIGR01192; EC 3.6.3.42; HMM-score: 326.2)
  Cellular processes Cellular processes Biosynthesis of natural products NHLM bacteriocin system ABC transporter, ATP-binding protein (TIGR03797; HMM-score: 313.1)
  Metabolism Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system ABC transporter, ATP-binding protein (TIGR03797; HMM-score: 313.1)
  thiol reductant ABC exporter, CydD subunit (TIGR02857; HMM-score: 313)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR01842; HMM-score: 277.1)
  thiol reductant ABC exporter, CydC subunit (TIGR02868; HMM-score: 250)
  Metabolism Transport and binding proteins Other multi drug resistance-associated protein (MRP) (TIGR00957; HMM-score: 182.6)
  Metabolism Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04520; EC 3.6.3.-; HMM-score: 147.1)
  Metabolism Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04521; EC 3.6.3.-; HMM-score: 139.7)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, ATP-binding protein (TIGR00972; EC 3.6.3.27; HMM-score: 135.8)
  Metabolism Transport and binding proteins Anions phosphonate ABC transporter, ATP-binding protein (TIGR02315; EC 3.6.3.28; HMM-score: 123.8)
  Metabolism Transport and binding proteins Amino acids, peptides and amines glycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 123.1)
  Cellular processes Cellular processes Toxin production and resistance putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 118.3)
  Metabolism Transport and binding proteins Unknown substrate putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 118.3)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids ABC transporter, ATP-binding subunit, PQQ-dependent alcohol dehydrogenase system (TIGR03864; HMM-score: 117.9)
  ABC exporter ATP-binding subunit, DevA family (TIGR02982; HMM-score: 113.2)
  Metabolism Transport and binding proteins Other thiamine ABC transporter, ATP-binding protein (TIGR01277; EC 3.6.3.-; HMM-score: 112.9)
  Metabolism Transport and binding proteins Amino acids, peptides and amines putative 2-aminoethylphosphonate ABC transporter, ATP-binding protein (TIGR03265; HMM-score: 112.8)
  Cellular processes Cellular processes Cell division cell division ATP-binding protein FtsE (TIGR02673; HMM-score: 109.4)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking lipoprotein releasing system, ATP-binding protein (TIGR02211; EC 3.6.3.-; HMM-score: 108.5)
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel import ATP-binding protein NikE (TIGR02769; EC 3.6.3.24; HMM-score: 102.5)
  Metabolism Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtE (TIGR03410; HMM-score: 101.7)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, ATP-binding protein (TIGR00968; EC 3.6.3.25; HMM-score: 100.2)
  D-methionine ABC transporter, ATP-binding protein (TIGR02314; EC 3.6.3.-; HMM-score: 99.5)
  2-aminoethylphosphonate ABC transport system, ATP-binding component PhnT (TIGR03258; HMM-score: 97)
  Metabolism Transport and binding proteins Other pigment precursor permease (TIGR00955; HMM-score: 96.4)
  Metabolism Transport and binding proteins Other daunorubicin resistance ABC transporter, ATP-binding protein (TIGR01188; HMM-score: 95.5)
  Metabolism Transport and binding proteins Anions molybdate ABC transporter, ATP-binding protein (TIGR02142; EC 3.6.3.29; HMM-score: 94.7)
  Metabolism Transport and binding proteins Anions cystic fibrosis transmembrane conductor regulator (CFTR) (TIGR01271; EC 3.6.3.49; HMM-score: 94.2)
  Metabolism Transport and binding proteins Amino acids, peptides and amines cyclic peptide transporter (TIGR01194; HMM-score: 92.9)
  Metabolism Transport and binding proteins Other cyclic peptide transporter (TIGR01194; HMM-score: 92.9)
  proposed F420-0 ABC transporter, ATP-binding protein (TIGR03873; HMM-score: 91.3)
  Metabolism Transport and binding proteins Anions nitrate ABC transporter, ATP-binding proteins C and D (TIGR01184; HMM-score: 90.2)
  Metabolism Transport and binding proteins Other nitrate ABC transporter, ATP-binding proteins C and D (TIGR01184; HMM-score: 90.2)
  Metabolism Transport and binding proteins Amino acids, peptides and amines polyamine ABC transporter, ATP-binding protein (TIGR01187; EC 3.6.3.31; HMM-score: 90.1)
  ectoine/hydroxyectoine ABC transporter, ATP-binding protein EhuA (TIGR03005; HMM-score: 85.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other FeS assembly ATPase SufC (TIGR01978; HMM-score: 84.1)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides LPS export ABC transporter ATP-binding protein (TIGR04406; HMM-score: 83)
  Metabolism Transport and binding proteins Other LPS export ABC transporter ATP-binding protein (TIGR04406; HMM-score: 83)
  Metabolism Transport and binding proteins Cations and iron carrying compounds cobalt ABC transporter, ATP-binding protein (TIGR01166; HMM-score: 82.5)
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel import ATP-binding protein NikD (TIGR02770; HMM-score: 81.8)
  Metabolism Transport and binding proteins Amino acids, peptides and amines choline ABC transporter, ATP-binding protein (TIGR03415; HMM-score: 80.7)
  gliding motility-associated ABC transporter ATP-binding subunit GldA (TIGR03522; HMM-score: 80.2)
  lantibiotic protection ABC transporter, ATP-binding subunit (TIGR03740; HMM-score: 75.7)
  Cellular processes Cellular processes Other nodulation ABC transporter NodI (TIGR01288; HMM-score: 75.1)
  Metabolism Transport and binding proteins Other nodulation ABC transporter NodI (TIGR01288; HMM-score: 75.1)
  Metabolism Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtD (TIGR03411; HMM-score: 72.9)
  phosphonate C-P lyase system protein PhnL (TIGR02324; HMM-score: 70.4)
  Metabolism Transport and binding proteins Unknown substrate anchored repeat-type ABC transporter, ATP-binding subunit (TIGR03771; HMM-score: 68.9)
  Metabolism Energy metabolism Methanogenesis methyl coenzyme M reductase system, component A2 (TIGR03269; HMM-score: 67.3)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 66.9)
  Metabolism Transport and binding proteins Other heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 66.9)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-xylose ABC transporter, ATP-binding protein (TIGR02633; EC 3.6.3.17; HMM-score: 66)
  Metabolism Transport and binding proteins Other rim ABC transporter (TIGR01257; HMM-score: 60.7)
  Metabolism Transport and binding proteins Other pleiotropic drug resistance family protein (TIGR00956; HMM-score: 56.7)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids peroxysomal long chain fatty acyl transporter (TIGR00954; HMM-score: 54.1)
  ATP-binding cassette protein, ChvD family (TIGR03719; HMM-score: 49.2)
  Metabolism Central intermediary metabolism Phosphorus compounds phosphonate C-P lyase system protein PhnK (TIGR02323; HMM-score: 40.8)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit A (TIGR00630; EC 3.1.25.-; HMM-score: 37.1)
  Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 13.7)
  Genetic information processing Protein synthesis Translation factors ribosome small subunit-dependent GTPase A (TIGR00157; EC 3.6.-.-; HMM-score: 13.4)
  Genetic information processing Protein synthesis Other ribosome biogenesis GTPase YqeH (TIGR03597; HMM-score: 13.4)
  Metabolism Central intermediary metabolism Sulfur metabolism adenylyl-sulfate kinase (TIGR00455; EC 2.7.1.25; HMM-score: 12.5)
  rad50 (TIGR00606; HMM-score: 12.2)
  Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02168; HMM-score: 9.7)
  Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02168; HMM-score: 9.7)
• TheSEED: see SAUSA300_1847
• PFAM:
  ABC_membrane (CL0241) ABC_membrane; ABC transporter transmembrane region (PF00664; HMM-score: 163)
  and 11 more
  P-loop_NTPase (CL0023) ABC_tran; ABC transporter (PF00005; HMM-score: 120.5)
  SMC_N; RecF/RecN/SMC N terminal domain (PF02463; HMM-score: 32.4)
  RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 22.1)
  AAA_16; AAA ATPase domain (PF13191; HMM-score: 15.9)
  NTPase_1; NTPase (PF03266; HMM-score: 15)
  MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 14.3)
  ABC_membrane (CL0241) ABC_membrane_3; ABC transporter transmembrane region (PF13748; HMM-score: 13.9)
  P-loop_NTPase (CL0023) AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 13.3)
  ABC_ATPase; Predicted ATPase of the ABC class (PF09818; HMM-score: 12.5)
  no clan defined Prp19; Prp19/Pso4-like (PF08606; HMM-score: 11.8)
  DUF2207; Predicted membrane protein (DUF2207) (PF09972; HMM-score: 5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 6
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.013654
  • TAT(Tat/SPI): 0.000143
  • LIPO(Sec/SPII): 0.003511
• predicted transmembrane helices (TMHMM): 5

⊟Accession numbers

• GI: 446519892 NCBI
• RefSeq: WP_000597238 NCBI
• UniProt: see SAUSA300_1847

⊟Protein sequence

• MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGVINNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYNHLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLDVKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAIEDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSITVGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIEIKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYDVTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATDEEVVEAAKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSALDLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGHIVETGTHRELIAKQGAYEHLYSIQNL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

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⊟Literature

⊟References

⊟Relevant publications