PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS10830 [old locus tag: SAUSA300_1971 ]
• pan locus tag^?: SAUPAN005160000
• symbol: SAUSA300_RS10830
• pan gene symbol^?: —
• synonym:
• product: toxin MazF

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS10830 [old locus tag: SAUSA300_1971 ]
• symbol: SAUSA300_RS10830
• product: toxin MazF
• replicon: chromosome
• strand: +
• coordinates: 2125708..2126421
• length: 714
• essential: unknown

⊟Accession numbers

• Location: NC_007793 (2125708..2126421) NCBI
• BioCyc: SAUSA300_RS10830 BioCyc
• MicrobesOnline: see SAUSA300_1971

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACGCATAATATAGAAAAACGCATTAATAAATTAAAAACTTCTGGAAATCCAAAATTT
  AAAAAATTAGATTCAGATATTCACTATTTACTCAAGAGATTTGAAGGTGAAAAAAACCAT
  AAAGGTTTTTATCCAAAGTTTAAACAAGGAGAAATAGTTTTTGTAGATTTCGGTATAAAC
  GTTAATAAAGAATTCTCTAATTCACACTTTGCAATAGTGATGAATAAAAATGATTCTAAT
  ACGGAAGATATAGTAAATGTTATTCCCTTATCTTCTAAAGAAAACAAAAAGTATTTAAAG
  ATGAATTTTGATTTGAAATGGGAGTATTATTTAAGATTGTTTTTAAATTTAATTAGCGCG
  CAAAATAATTCAGCTATATTAAAAGAAGTTTTCGATAAAAAATACCAAAAAAACAACACA
  GAATTCATCACTAAAGATTATTTTAGTGAATTTATATCTGATAGTTTAGAAATTGAAAAT
  AAATTAAATAAAATTGACAGAAACATTAATAACATAGTATCAGCAATTGATAAGGTAAAA
  AAATTAAAAGGTAATAGTTACGCTTGCATAAATTCTTTCCAGCCGATTAGTAAGTTTCGC
  ATAAGAAAAGTTTTACCCCAAAAAATTAAAAATCCAGTAATAGATTCTTCGGATATTATG
  TTACTGATAAATAGAATTAATAATAATATATTGCAGATTCCTGATATAAGATGA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS10830 [old locus tag: SAUSA300_1971 ]
• symbol: SAUSA300_RS10830
• description: toxin MazF
• length: 237
• theoretical pI: 10.2531
• theoretical MW: 27861
• GRAVY: -0.559072

⊟Function

• TIGRFAM:
• TheSEED: see SAUSA300_1971
• PFAM:
  CcdB_PemK (CL0624) PemK_toxin; PemK-like, MazF-like toxin of type II toxin-antitoxin system (PF02452; HMM-score: 34.1)
  and 6 more
  no clan defined F_actin_bind; F-actin binding (PF08919; HMM-score: 13.5)
  XhlA; Haemolysin XhlA (PF10779; HMM-score: 12.9)
  DUF16; Protein of unknown function DUF16 (PF01519; HMM-score: 12.2)
  P-loop_NTPase (CL0023) DUF87; Domain of unknown function DUF87 (PF01935; HMM-score: 12.2)
  Alk_phosphatase (CL0088) Phosphodiest; Type I phosphodiesterase / nucleotide pyrophosphatase (PF01663; HMM-score: 12)
  EF_hand (CL0220) Dockerin_1; Dockerin type I repeat (PF00404; HMM-score: 11.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004734
  • TAT(Tat/SPI): 0.000271
  • LIPO(Sec/SPII): 0.000539
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 486200495 NCBI
• RefSeq: WP_001549185 NCBI
• UniProt: see SAUSA300_1971

⊟Protein sequence

• MTHNIEKRINKLKTSGNPKFKKLDSDIHYLLKRFEGEKNHKGFYPKFKQGEIVFVDFGINVNKEFSNSHFAIVMNKNDSNTEDIVNVIPLSSKENKKYLKMNFDLKWEYYLRLFLNLISAQNNSAILKEVFDKKYQKNNTEFITKDYFSEFISDSLEIENKLNKIDRNINNIVSAIDKVKKLKGNSYACINSFQPISKFRIRKVLPQKIKNPVIDSSDIMLLINRINNNILQIPDIR

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications