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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS12335 [old locus tag: SAUSA300_2234 ]
- pan locus tag?: SAUPAN005745000
- symbol: SAUSA300_RS12335
- pan gene symbol?: —
- synonym:
- product: purine nucleosidase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS12335 [old locus tag: SAUSA300_2234 ]
- symbol: SAUSA300_RS12335
- product: purine nucleosidase
- replicon: chromosome
- strand: -
- coordinates: 2400465..2401406
- length: 942
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (2400465..2401406) NCBI
- BioCyc: SAUSA300_RS12335 BioCyc
- MicrobesOnline: see SAUSA300_2234
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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901GTGACTAAGAAAGTTTATTTTAACCACGATGGTGGTGTAGATGATTTAGTATCTCTATTT
TTATTATTACAAATGGAAAACGTTCAATTGATAGGGGTCAGTACAATTGGTGCTGATTGT
TATTTAGAGCCATCTTTGAGCGCATCAGTAAAAATTATTAATCGTTTTTCAAATGAAGAT
ATTCAAGTTGCGCCATCATATGAACGAGGAAAAAATCCATTTCCTAAAGAATGGCGTATG
CATGCCTTTTTTATGGACGCATTGCCAATTTTAAATGAGCCAGTCAAACATGTTGCTTCA
AATGTGAGCGACAAAGAAGCCTTTGAAGACATTATTCAAACTTTAAAGAGACAATCAGAA
AAAGTAACATTATTATTTACAGGCCCGCTTACAGATTTAGCAAAAGCACTACAAAAAGAT
TCATCTATCGTTCAGTATATAGAAAAATTAGTTTGGATGGGTGGCACCTTTTTACCAAAA
GGAAATGTTGAAGAACCTGAGCATGATGGTTCTGCAGAATGGAATGCATATTGGGATCCA
GAAGCGGTTAAAATTGTTTTTGATAGCGATATAGAGATTGATATGGTTGCTTTAGAAAGT
ACGAATCAAGTACCGCTAACGTTAGATGTTAGACAAAGATGGGCGAATGAACGCCAATAT
ACGGGCATTGATTTTTTAGGAGTAAGTTATGCAGCGGTACCACCATTAACACACTTTATA
ACAAATTCTACTTACTTTTTATGGGATGTTTTAACGACTGCTTATATTGGTAACAAGGAC
TTGGTTCATTCAATTGAGAAAAAAGTCGATGTAATAAGTTATGGACCAAGTCAAGGTAAG
ACATTTGAGTGTAAAGATGGGCGCAAAATTAATGTCATAAATCATGTAGATAACAACGCA
TTTTTTGATTATATAACTGCACTTGCTAAAAAAGTAAATTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS12335 [old locus tag: SAUSA300_2234 ]
- symbol: SAUSA300_RS12335
- description: purine nucleosidase
- length: 313
- theoretical pI: 4.78529
- theoretical MW: 35361.9
- GRAVY: -0.19393
⊟Function[edit | edit source]
- TIGRFAM: Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone O-succinylbenzoate-CoA ligase (TIGR01923; EC 6.2.1.26; HMM-score: 13.8)
- TheSEED: see SAUSA300_2234
- PFAM: no clan defined IU_nuc_hydro; Inosine-uridine preferring nucleoside hydrolase (PF01156; HMM-score: 172.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.066744
- TAT(Tat/SPI): 0.002157
- LIPO(Sec/SPII): 0.005834
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446085561 NCBI
- RefSeq: WP_000163416 NCBI
- UniProt: see SAUSA300_2234
⊟Protein sequence[edit | edit source]
- MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEPSLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNVSDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPKGNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANERQYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGPSQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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