PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS12665 [old locus tag: SAUSA300_2292 ]
• pan locus tag^?: SAUPAN005845000
• symbol: SAUSA300_RS12665
• pan gene symbol^?: fni
• synonym:
• product: type 2 isopentenyl-diphosphate Delta-isomerase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS12665 [old locus tag: SAUSA300_2292 ]
• symbol: SAUSA300_RS12665
• product: type 2 isopentenyl-diphosphate Delta-isomerase
• replicon: chromosome
• strand: -
• coordinates: 2464602..2465651
• length: 1050
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (2464602..2465651) NCBI
• BioCyc: SAUSA300_RS12665 BioCyc
• MicrobesOnline: see SAUSA300_2292

⊟Phenotype

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⊟DNA sequence

• 1
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  1021
  
  ATGAGTGATTTTCAAAGAGAACAGAGAAAAAATGAACATGTTGAAATAGCAATGGCCCAA
  TCTGACGCAATGCATTCAGATTTTGATAAGATGCGTTTTGTGCATCATTCTATCCCATCA
  ATTAATGTAAATGATATCGATTTGACATCACAGACGCCTGACTTAACGATGGCATATCCG
  GTTTATATTAATGCAATGACGGGTGGTAGCGAGTGGACGAAAAACATCAATGAAAAGCTA
  GCTGTAGTTGCAAGAGAAACTGGCTTAGCGATGGCAGTTGGATCAACACATGCGGCATTG
  AGAAATCCACGCATGGCTGAGACGTTTACGATTGCGCGAAAAATGAATCCTGAAGGCATG
  ATTTTTAGCAATGTTGGTGCGGACGTACCAGTAGAAAAGGCTTTGGAAGCAGTTGAATTA
  CTTGAGGCACAAGCGTTACAAATCCATGTTAATTCTCCTCAAGAATTAGTTATGCCTGAA
  GGGAATCGTGAATTTGTGACTTGGTTAGATAATATAGCGTCGATTGTATCACGAGTGTCT
  GTTCCAGTCATTATAAAAGAAGTTGGATTTGGTATGAGCAAAGAATTAATGCATGACTTA
  CAACAAATAGGCGTCAAGTATGTCGATGTTAGTGGCAAAGGTGGTACTAACTTTGTAGAT
  ATTGAAAATGAACGTCGTGCAAATAAAGATATGGATTACTTATCATCATGGGGACAGTCT
  ACAGTTGAGTCATTACTTGAAACAACGGCTTATCAAAGCGAAATTTCAGTTTTCGCGAGT
  GGTGGTTTACGTACACCACTCGATGCAATTAAAAGTTTAGCACTTGGTGCAAAGGCAACT
  GGTATGTCACGTCCGTTTTTAAATCAAGTTGAAAATAATGGCATTGCACATACAGTAGCT
  TATGTAGAATCATTTATTGAACACATGAAATCAATAATGACGATGTTAGATGCTAAAAAT
  ATTGACGACTTAACACAAAAACAAATCGTATTTAGTCCGGAAATATTGTCATGGATAGAA
  CAACGTAACTTGAATATACATCGAGGATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS12665 [old locus tag: SAUSA300_2292 ]
• symbol: SAUSA300_RS12665
• description: type 2 isopentenyl-diphosphate Delta-isomerase
• length: 349
• theoretical pI: 5.0077
• theoretical MW: 38769.9
• GRAVY: -0.201719

⊟Function

• reaction:
  EC 5.3.3.2?  ExPASy
  Isopentenyl-diphosphate Delta-isomerase Isopentenyl diphosphate = dimethylallyl diphosphate
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other isopentenyl-diphosphate delta-isomerase, type 2 (TIGR02151; EC 5.3.3.2; HMM-score: 426.4)
  and 6 more
  heme/flavin dehydrogenase, mycofactocin system (TIGR03966; EC 1.-.-.-; HMM-score: 28.6)
  Metabolism Energy metabolism Other L-lactate oxidase (TIGR02708; EC 1.1.3.-; HMM-score: 16.2)
  Metabolism Energy metabolism Other deoxyribose-phosphate aldolase (TIGR00126; EC 4.1.2.4; HMM-score: 15.3)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other deoxyribose-phosphate aldolase (TIGR00126; EC 4.1.2.4; HMM-score: 15.3)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis inosine-5'-monophosphate dehydrogenase (TIGR01302; EC 1.1.1.205; HMM-score: 14.8)
  putative enoyl-[acyl-carrier-protein] reductase II (TIGR03151; EC 1.3.1.-; HMM-score: 13.9)
• TheSEED: see SAUSA300_2292
• PFAM:
  TIM_barrel (CL0036) FMN_dh; FMN-dependent dehydrogenase (PF01070; HMM-score: 85.5)
  and 3 more
  Glu_synthase; Conserved region in glutamate synthase (PF01645; HMM-score: 25.8)
  IMPDH; IMP dehydrogenase / GMP reductase domain (PF00478; HMM-score: 17.7)
  DHO_dh; Dihydroorotate dehydrogenase (PF01180; HMM-score: 13.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: FMN, Mg²⁺, NADPH
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008927
  • TAT(Tat/SPI): 0.001746
  • LIPO(Sec/SPII): 0.001146
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447202125 NCBI
• RefSeq: WP_001279381 NCBI
• UniProt: see SAUSA300_2292

⊟Protein sequence

• MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVNDIDLTSQTPDLTMAYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRNPRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPEGNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNFVDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALGAKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPEILSWIEQRNLNIHRG

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications