PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS12845 [old locus tag: SAUSA300_2324 ]
• pan locus tag^?: SAUPAN005897000
• symbol: SAUSA300_RS12845
• pan gene symbol^?: scrA
• synonym:
• product: PTS sucrose transporter subunit IIBC

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS12845 [old locus tag: SAUSA300_2324 ]
• symbol: SAUSA300_RS12845
• product: PTS sucrose transporter subunit IIBC
• replicon: chromosome
• strand: -
• coordinates: 2497831..2499273
• length: 1443
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (2497831..2499273) NCBI
• BioCyc: SAUSA300_RS12845 BioCyc
• MicrobesOnline: see SAUSA300_2324

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  481
  541
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  841
  901
  961
  1021
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  1141
  1201
  1261
  1321
  1381
  1441
  
  ATGAATTATAAGCAATCCGCAGAAGAAATTTTGAACGCGATAGGCGGAGAAGAGAATTTA
  GATGCAATGGCGCATTGTGCAACGAGACTACGATTAGTTTTAAATGATGAAAGTTTAGTA
  AATGAAGAGGCGCTAAACAATATGGATGTAGTTAAAGGGACGTTTTCTACTGGGGGACAA
  TACCAAATTATTATTGGGTCTGGTACAGTCAATAAAGTATTTAGTGAACTGGAAAAATTA
  ACTGGAAAAGAAGCATCAACCACTTCGGAAGTCAAAGCACAATCTGCTAAAAATATGAAT
  CCGTTACAGCGATTTGTAAAAATGCTTTCAGATATCTTTGTTCCGATTATACCAGCCATC
  GTTGCTGGTGGTTTATTAATGGGGTTAAATAACATTTTGACTGCGAAAGATTTATTCTTT
  TCAGGTAAATCATTGATAGATGTATATAGTCAATTTGCTGGATTAGCTGAAATGATAAAT
  GTTTTTGCGAATGCACCATTTACATTATTACCAATTTTAATTGGATTTAGTGCAGCAAAA
  CGCTTTGGTGGCAATCCATTTTTAGGTGCTGCATTAGGTATGATACTAGTTCATCCATCG
  CTAATGAGCGCATACGATTTCCCAAAAGCAGTTGAAGCAGGTAAGGCTATTCCATATTGG
  GATGTTTTTGGTTTGCATATTAATCAAGTAGGTTATCAAGGACAAGTGTTACCTATGCTT
  GTAGCAGCTTATATCTTAGCCTCAATTGAAAAAGGGTTACGCAAAGTTATTCCAACGGTG
  TTAGATAATTTGTTAACACCATTGTTATCTATTTTTATAACAGCATTTCTAACATTTTCA
  TTTGTAGGTCCAATCACTCGACAATTAGGTTACTGGTTATCAGATGGTTTAACATGGCTT
  TATGAATTTGGTGGTGCAATTGGTGGATTAATATTCGGATTATTGTATGCTCCGATTGTT
  ATTACAGGTATGCATCATAGCTTTATAGCTGTAGAAACGACATTAATTGCAGATGCCACT
  AAAACGGGTGGATCATTTATATTCCCGATTGCGACAATGTCTAATGTTGCACAAGGTGGT
  GCAGCAATTGCAGCGTTCTTTATTATTAAACAAAATAAGAAGTTAAAAGGTGTGGCATCT
  GCCGCAGGTATTTCAGCATTACTTGGTATTACAGAACCGGCTATGTTTGGTGTTAACTTA
  AAACTAAGATATCCATTTATTGGCGCTATCGTTGGATCAGGTATTGGTTCAGCATATATT
  GCTTTCTTCAAGGTTAAAGCAATCGCATTAGGAACTGCTGGATTGCCAGGATTTATTTCA
  ATCAATCCAGTACATGCAGGATGGTTACACTACTTTGTTGGTATGACAATATCATTCATC
  ATTGCTATAACAGTTACTTTAATTTTATCTAAAAGAAAAGCAAATAAAGAAGTTGTAGAA
  TAA
  60
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  1320
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  1440
  1443
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS12845 [old locus tag: SAUSA300_2324 ]
• symbol: SAUSA300_RS12845
• description: PTS sucrose transporter subunit IIBC
• length: 480
• theoretical pI: 9.25364
• theoretical MW: 51217.9
• GRAVY: 0.579583

⊟Function

• reaction:
  EC 2.7.1.69?  ExPASy
  Transferred entry: 2.7.1.191, 2.7.1.192, 2.7.1.193, 2.7.1.194, 2.7.1.195, 2.7.1.196, 2.7.1.197, 2.7.1.198, 2.7.1.199, 2.7.1.200, 2.7.1.201, 2.7.1.202, 2.7.1.203, 2.7.1.204, 2.7.1.205, 2.7.1.206, 2.7.1.207 and 2.7.1.208
• TIGRFAM:
  PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 676.1)
  PTS system, trehalose-specific IIBC component (TIGR01992; EC 2.7.1.69; HMM-score: 553.8)
  and 10 more
  PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 369.4)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 273.1)
  Signal transduction Signal transduction PTS PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 273.1)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 86.5)
  Signal transduction Signal transduction PTS PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 86.5)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, N-acetylglucosamine-specific IIBC component (TIGR01998; EC 2.7.1.69; HMM-score: 41.7)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, alpha-glucoside-specific IIBC component (TIGR02005; EC 2.7.1.69; HMM-score: 38.4)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific IIBC component (TIGR02004; EC 2.7.1.69; HMM-score: 35.9)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-specific IIBC component (TIGR02002; EC 2.7.1.69; HMM-score: 27.1)
  glycopeptide, sublancin family (TIGR04196; HMM-score: 13.8)
• TheSEED: see SAUSA300_2324
• PFAM:
  PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 206.5)
  and 2 more
  no clan defined PTS_EIIB; phosphotransferase system, EIIB (PF00367; HMM-score: 52.3)
  GPCR_A (CL0192) Ceramidase; Ceramidase (PF05875; HMM-score: 12.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 10
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.011369
  • TAT(Tat/SPI): 0.000773
  • LIPO(Sec/SPII): 0.00117
• predicted transmembrane helices (TMHMM): 8

⊟Accession numbers

• GI: 447030870 NCBI
• RefSeq: WP_001108126 NCBI
• UniProt: see SAUSA300_2324

⊟Protein sequence

• MNYKQSAEEILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEALNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPLQRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMINVFANAPFTLLPILIGFSAAKRFGGNPFLGAALGMILVHPSLMSAYDFPKAVEAGKAIPYWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAFLTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETTLIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITEPAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLHYFVGMTISFIIAITVTLILSKRKANKEVVE

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA, ScrR see SAUSA300_2324

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications