PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS12890 [old locus tag: SAUSA300_2333 ]
• pan locus tag^?: SAUPAN005915000
• symbol: SAUSA300_RS12890
• pan gene symbol^?: narK
• synonym:
• product: MFS transporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS12890 [old locus tag: SAUSA300_2333 ]
• symbol: SAUSA300_RS12890
• product: MFS transporter
• replicon: chromosome
• strand: -
• coordinates: 2508369..2509538
• length: 1170
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (2508369..2509538) NCBI
• BioCyc: SAUSA300_RS12890 BioCyc
• MicrobesOnline: see SAUSA300_2333

⊟Phenotype

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⊟DNA sequence

• 1
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  1141
  
  ATGTACAAAACAAAAGGTGGCTTTCAACTTACATTACAAACATTAAGTTTAGTGGTTGGG
  TTTATGGCTTGGAGTATAATTGCGCCATTAATGCCCTTTATTAAACAAGATGTCAATGTT
  ACTGAAGGTCAAATATCAATCATTTTAGCGATACCAGTTATTTTGGGATCGGTGCTCCGT
  GTGCCATTTGGTTATTTAACAAACATTGTTGGCGCTAAATGGGTATTCTTTACTAGTTTT
  ATCGTATTGTTATTCCCGATATTTTTCTTAAGCCAAGCACAAACACCGGGTATGTTAATG
  GCTTCAGGATTTTTCCTTGGTGTAGGTGGTGCAATTTTCTCAGTTGGTGTTACATCAGTT
  CCTAAATATTTCCCTAAAGAAAAAGTAGGTCTAGCAAATGGTATTTATGGTATGGGAAAT
  ATCGGTACAGCAGTTTCTTCATTTTTAGCACCACCGATAGCGGGTATTATTGGTTGGCAA
  ACAACAGTTAGAAGTTACTTAATTATTATCGCTTTATTTGCATTAATTATGTTCATTTTT
  GGTGACACACAAGAACGTAAAATTAAAGTACCATTAATGGCTCAAATGAAAACATTATCT
  AAAAACTACAAATTATATTACTTAAGTTATTGGTATTTTATTACTTTTGGTGCTTTTGTA
  GCATTTGGTATTTTCTTACCTAACTACTTAGTAAATCATTTTGGAATTGACAAAGTAGAT
  GCTGGTATTCGATCAGGTGTATTCATTGCGCTGGCAACATTCTTAAGACCAATAGGTGGC
  ATTTTAGGTGATAAATTTAATGCAGTTAAAGTATTGATGATTGATTTTGTTGTTATGATT
  ATCGGTGCCATTATTTTAGGTATTTCAGACCATATCGCATTATTCACTGTAGGCTGTTTA
  ACAATAAGTATTTGTGCAGGTATTGGTAACGGCTTAATCTTCAAATTAGTACCATCATAC
  TTCTTAAATGAAGCGGGATCCGCAAATGGTATCGTATCAATGATGGGTGGTTTAGGAGGA
  TTCTTCCCACCACTAGTAATCACGTACGTAGCTAATTTAACAGGATCAAGTCATTTAGCA
  TTTATTTTCTTAGCGGTATTTGGATGTATTGCATTATTTACCATGCGTCATTTATACCAA
  AAAGAATATGGCTCATTGAAAAACGGTTGA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS12890 [old locus tag: SAUSA300_2333 ]
• symbol: SAUSA300_RS12890
• description: MFS transporter
• length: 389
• theoretical pI: 9.92595
• theoretical MW: 42148.2
• GRAVY: 0.921851

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Anions nitrite transporter (TIGR00886; HMM-score: 197.7)
  and 9 more
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 58.2)
  Metabolism Transport and binding proteins Other cyanate transporter (TIGR00896; HMM-score: 58)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 50.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 44.7)
  multidrug resistance protein (TIGR00880; HMM-score: 36.3)
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 22.1)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 22.1)
  oxalate/formate antiporter (TIGR04259; HMM-score: 21.7)
  sodium-independent organic anion transporter (TIGR00805; HMM-score: 15.3)
• TheSEED: see SAUSA300_2333
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 150.3)
  and 3 more
  OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 19)
  NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 11.5)
  MFS (CL0015) MFS_3; Transmembrane secretion effector (PF05977; HMM-score: 8.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.070673
  • TAT(Tat/SPI): 0.001376
  • LIPO(Sec/SPII): 0.020064
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 446200703 NCBI
• RefSeq: WP_000278558 NCBI
• UniProt: see SAUSA300_2333

⊟Protein sequence

• MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMASGFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQTTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGAFVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDFVVMIIGAIILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFLNEAGSANGIVSMMGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKNG

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: NreC*, Rex see SAUSA300_2333

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications