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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS13725 [old locus tag: SAUSA300_2473 ]
  • pan locus tag?: SAUPAN006189000
  • symbol: SAUSA300_RS13725
  • pan gene symbol?:
  • synonym:
  • product: alpha/beta hydrolase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS13725 [old locus tag: SAUSA300_2473 ]
  • symbol: SAUSA300_RS13725
  • product: alpha/beta hydrolase
  • replicon: chromosome
  • strand: -
  • coordinates: 2669999..2670967
  • length: 969
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCGTAAAAAATGGTCTACACTTGCGTTTGGATTTTTAGTTGCAGCATACGCACATATT
    AGAATTAAAGAAAAACGCAGTGTGAAAAGTTATATGTTAGAACAAGGTATACGATTATCT
    AGAGCTAAGCGTCGTTTTATGTATAAAGAAGAAGCGATGAAAGCATTAGAAAAAATGGCG
    CCACAGACAGCAGGCGAATATGAGGGAACCAATTATCAGTTTAAGATGCCAGTAAAAGTG
    GATAAGCACTTCGGTTCAACCGTTTATACCGTTAACGATAAACAAGATAAGCATCAACGC
    GTTGTATTATATGCACATGGAGGCGCATGGTTCCAAGACCCACTCAAAATTCATTTCGAA
    TTTATTGATGAACTTGCAGAAACACTCAATGCTAAAGTCATCATGCCAGTATATCCGAAG
    ATTCCGCATCAAGATTATCAAGCGACGTATGTGCTTTTTGAAAAGTTGTACCATGATTTA
    TTGAATCAAGTAGCAGATTCTAAACAAATCGTTGTAATGGGTGACTCTGCGGGCGGTCAA
    ATTGCTTTATCATTTGCTCAATTGTTAAAAGAAAAACATATTGTGCAACCAGGACATATT
    GTATTAATTTCACCAGTTTTAGATGCAACGATGCAGCATCCTGAAATTCCTGACTACTTA
    AAGAAAGACCCAATGGTAGGTGTGGATGGCAGTGTGTTCTTAGCTGAACAATGGGCAGGG
    GACACACCTTTAGATAACTACAAAGTATCACCAATTAATGGTGACTTAGATGGTTTAGGT
    CGTATTACATTAACTGTTGGTACAAAAGAAGTACTGTACCCAGATGCTTTGAACTTATCG
    CAATTGTTGAGTGCGAAAGGTATCGAACATGACTTTATACCTGGATATTACCAATTCCAT
    ATTTATCCAGTATTTCCGATACCAGAGCGACGACGCTTTTTATACCAAGTGAAAAATATT
    ATTAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    969

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS13725 [old locus tag: SAUSA300_2473 ]
  • symbol: SAUSA300_RS13725
  • description: alpha/beta hydrolase
  • length: 322
  • theoretical pI: 9.11945
  • theoretical MW: 36847.4
  • GRAVY: -0.26087

Function[edit | edit source]

  • TIGRFAM:
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, Rv1855c family (TIGR03560; HMM-score: 11.9)
  • TheSEED: see SAUSA300_2473
  • PFAM:
    AB_hydrolase (CL0028) Abhydrolase_3; alpha/beta hydrolase fold (PF07859; HMM-score: 104.8)
    and 9 more
    Say1_Mug180; Steryl acetyl hydrolase (PF10340; HMM-score: 55.6)
    Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 19.7)
    Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 18.6)
    Thioesterase; Thioesterase domain (PF00975; HMM-score: 17.1)
    Esterase; Putative esterase (PF00756; HMM-score: 15.2)
    AXE1; Acetyl xylan esterase (AXE1) (PF05448; HMM-score: 14.1)
    no clan defined UPF0240; Uncharacterised protein family (UPF0240) (PF06784; HMM-score: 13.4)
    AB_hydrolase (CL0028) BAAT_C; BAAT / Acyl-CoA thioester hydrolase C terminal (PF08840; HMM-score: 13.1)
    Lipase_3; Lipase (class 3) (PF01764; HMM-score: 13)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.224571
    • TAT(Tat/SPI): 0.004058
    • LIPO(Sec/SPII): 0.162038
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKRRFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVKVDKHFGSTVYTVNDKQDKHQRVVLYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDLLNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPDYLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDALNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]