From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS13970 [old locus tag: SAUSA300_2517 ]
  • pan locus tag?: SAUPAN006255000
  • symbol: SAUSA300_RS13970
  • pan gene symbol?: —
  • synonym:
  • product: amidohydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS13970 [old locus tag: SAUSA300_2517 ]
  • symbol: SAUSA300_RS13970
  • product: amidohydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 2719713..2720723
  • length: 1011
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAAGTATTACGTTTGAAGAACATTATGTCATTGAAGATATTCAAAAAGAAACGATG
    AATGCGATATCAGCAGATCCTAAAGGTGTACCGATGAAAGTAATGTTAGAAGGCCTTGAA
    AAAAAGACAGGTTTTACAAATGCCGACGAATTATCACATCATGATGAACGTATCCAATTT
    ATGAATAATCAAGACGTTCAAATTCAAGTCTTATCTTATGGAAATGGTTCTCCTTCAAAT
    TTGGTTGGTCAGAAAGCCATTGAATTATGTCAAAAAGCAAATGATCAATTGGCAAACTAT
    ATTGCACAATATCCCAATCGCTTTGTAGGCTTTGCAACTTTACCTATCAATGAGCCTGAA
    GCCGCAGCGCGAGAATTCGAACGTTGCATCAATGATTTAGGATTCAAAGGTGCGCTCATT
    ATGGGACGTGCACAAGATGGTTTTCTTGATCAAGACAAATATGACATTATTTTCAAAACA
    GCTGAAAATTTAGACGTACCGATTTATCTACATCCCGCGCCAGTTAACAGTGACATTTAT
    CAATCATACTATAAAGGAAATTATCCTGAAGTAACTGCGGCAACATTTGCTTGTTTTGGT
    TATGGTTGGCACATTGATGTCGGCATTCATGCAATACATCTAGTATTATCTGGTATTTTT
    GATCGTTATCCAAAGTTAAATATGATTATTGGACATTGGGGTGAGTTTATCCCATTCTTC
    TTAGAACGTATGGATGAAGCTTTATTCGCTGAACATTTGAACCACCCTGTAAGCTATTAC
    TTTAAAAATAATTTTTATATCACACCGAGTGGCATGTTAACGAAGCCACAGTTTGATTTA
    GTCAAGAAAGAAGTAGGTATTGATAGAATTCTTTATGCTGCTGATTATCCATATATTGAG
    CCTGAAAAATTAGGTGTATTTTTAGATGAACTGGGTTTAACAGATGAAGAAAAAGAGAAA
    ATAAGTTATACAAATGGTGCTAAATTATTAGGTTTATCACCTAACAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1011

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS13970 [old locus tag: SAUSA300_2517 ]
  • symbol: SAUSA300_RS13970
  • description: amidohydrolase
  • length: 336
  • theoretical pI: 4.70058
  • theoretical MW: 38164.1
  • GRAVY: -0.280952

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: see SAUSA300_2517
  • PFAM:
    Amidohydrolase (CL0034) Amidohydro_2; Amidohydrolase (PF04909; HMM-score: 148.5)
    and 1 more
    TatD_DNase; TatD related DNase (PF01026; HMM-score: 11.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006176
    • TAT(Tat/SPI): 0.000437
    • LIPO(Sec/SPII): 0.000858
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKSITFEEHYVIEDIQKETMNAISADPKGVPMKVMLEGLEKKTGFTNADELSHHDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIAQYPNRFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKTAENLDVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSGIFDRYPKLNMIIGHWGEFIPFFLERMDEALFAEHLNHPVSYYFKNNFYITPSGMLTKPQFDLVKKEVGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSPNN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAUSA300_RS13970&oldid=88365"