COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS14815 [old locus tag: SAUSA300_pUSA030016 ]
• pan locus tag^?:
• symbol: SAUSA300_RS14815
• pan gene symbol^?: —
• synonym:
• product: mannosyl-glycoprotein endo-beta-N-acetylglucosamidase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS14815 [old locus tag: SAUSA300_pUSA030016 ]
• symbol: SAUSA300_RS14815
• product: mannosyl-glycoprotein endo-beta-N-acetylglucosamidase
• replicon: pUSA03
• strand: +
• coordinates: 15827..16903
• length: 1077
• essential: unknown

⊟Accession numbers

• Location: NC_007792 (15827..16903) NCBI
• BioCyc: SAUSA300_RS14815 BioCyc
• MicrobesOnline: see SAUSA300_pUSA030016

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1021
  
  ATGTGGAGTGCTATTGCTAAAGGAATAACAATGCTGATGAAGAAAAAAGCATTATCCAAA
  ATATTAAAACCAGTAATAGCTATAGTAATAGGCTTTTTCCTTCTTATTTTTATAATGATT
  GCTTATCTAGTAGGATCTTTCACTAAAGAAATTAATGAGAGTGGCGAACAAAAAATGAAA
  GAGGCTGAATCAACAGCAACAGGTGCGTGTACTGGTGGAAGTGTTGACGGTTCGGGTATC
  TCTGCTTTTGAAAAAAACGCCAAAGGTGGCGCATTAGAAGGTAAAGGTAAGGATATGGTT
  AAAATTGCTAAAAAAAATAAAATACCACCAAAATTATTTATAGCAATTGTAGCTTCTGAG
  TCAGAATGGGGAAGAGGGGCAAATGCAACAAAACAAAAAAACCCCCTATCTATAATGGGG
  GCTGGCCCTCTACAAGTTTACCCTTCAATTGAAGAAGGACTTGATAAAGGTGCAAAAAAT
  TTATATGACTTATACATTTCTGAAGGTTTGACAACTCCCGAGAAAATCGGGCCAAAATAC
  GCACCAGTAGGCGCTAGTAATGACCCAGATGACTTAAATAGTAACTGGGTACCTACAGTT
  AAGAAAATCATGAAGTCATTCGGTGGCAAAGAGGCTAAATGTTCTACTGAAAGCAGTGGT
  GGAGGTTCAGACGGAAAAGGATTTGATTTTAAAGGAGAATTCCCTAAACCTGACAAATCG
  AAATATAACGGTCAATCTTATCCATGGGGACAGTGTACTTGGTATGTACATCAACGTAGA
  AAAGAAATTGGCAAACCAGTACCTTTAACTTGGGGTAATGGTGGGGACTGGGGCGATAAT
  GCAAAAGCGCAAGGCTGGGAAGTAGGTAGTAAGCCGAAAGCTGGCGCTGGCGCCTCTGTA
  AAACCCGGTAACTTTGGCGCACCACCACCGTATGGACACATCATGTTTGTCGAAAAAGTT
  AAAAAAGACGGTGGAATTGTAGTTAGTGAGGCCAATGTAAAAGGCCTAGGAGTTATAAGT
  TCAAGAGAATTTTCAAAAGCAGAAACACAACGTATGCAATTTATTTATGATAAGTAG
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⊟Protein

⊟General

• locus tag: SAUSA300_RS14815 [old locus tag: SAUSA300_pUSA030016 ]
• symbol: SAUSA300_RS14815
• description: mannosyl-glycoprotein endo-beta-N-acetylglucosamidase
• length: 358
• theoretical pI: 10.0211
• theoretical MW: 38301.8
• GRAVY: -0.391899

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Sporulation and germination putative sporulation protein YtaF (TIGR02840; HMM-score: 13.3)
  Metabolism Transport and binding proteins Amino acids, peptides and amines oligopeptide transporter, OPT family (TIGR00733; HMM-score: 11)
• TheSEED: see SAUSA300_pUSA030016
• PFAM:
  Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 58.1)
  and 2 more
  Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 39.1)
  no clan defined T2SSF; Type II secretion system (T2SS), protein F (PF00482; HMM-score: 7.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0.03
  • Cytoplasmic Membrane Score: 0.77
  • Cellwall Score: 9.14
  • Extracellular Score: 0.06
  • Internal Helix: 1
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006407
  • TAT(Tat/SPI): 0.000274
  • LIPO(Sec/SPII): 0.002706
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 446191530 NCBI
• RefSeq: WP_000269385 NCBI
• UniProt: see SAUSA300_pUSA030016

⊟Protein sequence

• MWSAIAKGITMLMKKKALSKILKPVIAIVIGFFLLIFIMIAYLVGSFTKEINESGEQKMKEAESTATGACTGGSVDGSGISAFEKNAKGGALEGKGKDMVKIAKKNKIPPKLFIAIVASESEWGRGANATKQKNPLSIMGAGPLQVYPSIEEGLDKGAKNLYDLYISEGLTTPEKIGPKYAPVGASNDPDDLNSNWVPTVKKIMKSFGGKEAKCSTESSGGGSDGKGFDFKGEFPKPDKSKYNGQSYPWGQCTWYVHQRRKEIGKPVPLTWGNGGDWGDNAKAQGWEVGSKPKAGAGASVKPGNFGAPPPYGHIMFVEKVKKDGGIVVSEANVKGLGVISSREFSKAETQRMQFIYDK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications