COLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 23-MAY-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_pUSA030016 [new locus tag: SAUSA300_RS14815 ]
• pan locus tag^?:
• symbol: traG
• pan gene symbol^?: —
• synonym:
• product: transfer complex protein TraG

⊟Additional information (user-provided)

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_pUSA030016 [new locus tag: SAUSA300_RS14815 ]
• symbol: traG
• product: transfer complex protein TraG
• replicon: pUSA03
• strand: +
• coordinates: 15827..16903
• length: 1077
• essential: unknown

⊟Accession numbers

• Gene ID: 3912758 NCBI
• RefSeq: YP_492702 NCBI
• BioCyc: see SAUSA300_RS14815
• MicrobesOnline: 1291495 MicrobesOnline

⊟Phenotype

⊟Additional information (user-provided)

⊟DNA sequence

• 1
  61
  121
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  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  
  ATGTGGAGTGCTATTGCTAAAGGAATAACAATGCTGATGAAGAAAAAAGCATTATCCAAA
  ATATTAAAACCAGTAATAGCTATAGTAATAGGCTTTTTCCTTCTTATTTTTATAATGATT
  GCTTATCTAGTAGGATCTTTCACTAAAGAAATTAATGAGAGTGGCGAACAAAAAATGAAA
  GAGGCTGAATCAACAGCAACAGGTGCGTGTACTGGTGGAAGTGTTGACGGTTCGGGTATC
  TCTGCTTTTGAAAAAAACGCCAAAGGTGGCGCATTAGAAGGTAAAGGTAAGGATATGGTT
  AAAATTGCTAAAAAAAATAAAATACCACCAAAATTATTTATAGCAATTGTAGCTTCTGAG
  TCAGAATGGGGAAGAGGGGCAAATGCAACAAAACAAAAAAACCCCCTATCTATAATGGGG
  GCTGGCCCTCTACAAGTTTACCCTTCAATTGAAGAAGGACTTGATAAAGGTGCAAAAAAT
  TTATATGACTTATACATTTCTGAAGGTTTGACAACTCCCGAGAAAATCGGGCCAAAATAC
  GCACCAGTAGGCGCTAGTAATGACCCAGATGACTTAAATAGTAACTGGGTACCTACAGTT
  AAGAAAATCATGAAGTCATTCGGTGGCAAAGAGGCTAAATGTTCTACTGAAAGCAGTGGT
  GGAGGTTCAGACGGAAAAGGATTTGATTTTAAAGGAGAATTCCCTAAACCTGACAAATCG
  AAATATAACGGTCAATCTTATCCATGGGGACAGTGTACTTGGTATGTACATCAACGTAGA
  AAAGAAATTGGCAAACCAGTACCTTTAACTTGGGGTAATGGTGGGGACTGGGGCGATAAT
  GCAAAAGCGCAAGGCTGGGAAGTAGGTAGTAAGCCGAAAGCTGGCGCTGGCGCCTCTGTA
  AAACCCGGTAACTTTGGCGCACCACCACCGTATGGACACATCATGTTTGTCGAAAAAGTT
  AAAAAAGACGGTGGAATTGTAGTTAGTGAGGCCAATGTAAAAGGCCTAGGAGTTATAAGT
  TCAAGAGAATTTTCAAAAGCAGAAACACAACGTATGCAATTTATTTATGATAAGTAG
  60
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  1077
  

⊟Protein

⊟General

• locus tag: SAUSA300_pUSA030016 [new locus tag: SAUSA300_RS14815 ]
• symbol: TraG
• description: transfer complex protein TraG
• length: 358
• theoretical pI: 10.0211
• theoretical MW: 38301.8
• GRAVY: -0.391899

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Sporulation and germination putative sporulation protein YtaF (TIGR02840; HMM-score: 13.3)
  Metabolism Transport and binding proteins Amino acids, peptides and amines oligopeptide transporter, OPT family (TIGR00733; HMM-score: 11)
• TheSEED  :
  • Secretory antigen SsaA-like protein transposon-related
• PFAM:
  Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 78.7)
  and 3 more
  Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 30.4)
  SLT; Transglycosylase SLT domain (PF01464; HMM-score: 12.1)
  no clan defined T2SSF; Type II secretion system (T2SS), protein F (PF00482; HMM-score: 6.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0.03
  • Cytoplasmic Membrane Score: 0.77
  • Cellwall Score: 9.14
  • Extracellular Score: 0.06
  • Internal Helix: 1
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.0001
  • Cytoplasmic Membrane Score: 0.705
  • Cell wall & surface Score: 0.0002
  • Extracellular Score: 0.2947
• LocateP: Lipid anchored
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPII)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: ATGACTG
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006407
  • TAT(Tat/SPI): 0.000274
  • LIPO(Sec/SPII): 0.002706
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 87159855 NCBI
• RefSeq: YP_492702 NCBI
• UniProt: A0A0H2XJH0 UniProt

⊟Additional information (user-provided)

⊟Protein sequence

• MWSAIAKGITMLMKKKALSKILKPVIAIVIGFFLLIFIMIAYLVGSFTKEINESGEQKMKEAESTATGACTGGSVDGSGISAFEKNAKGGALEGKGKDMVKIAKKNKIPPKLFIAIVASESEWGRGANATKQKNPLSIMGAGPLQVYPSIEEGLDKGAKNLYDLYISEGLTTPEKIGPKYAPVGASNDPDDLNSNWVPTVKKIMKSFGGKEAKCSTESSGGGSDGKGFDFKGEFPKPDKSKYNGQSYPWGQCTWYVHQRRKEIGKPVPLTWGNGGDWGDNAKAQGWEVGSKPKAGAGASVKPGNFGAPPPYGHIMFVEKVKKDGGIVVSEANVKGLGVISSREFSKAETQRMQFIYDK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: traA > traB > traC > traD > traE > traF > traG > traH > traI > traJ > traK

⊟Regulation

• regulator:

⊟Additional information (user-provided)

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Additional information (user-provided)

⊟Other information (user-provided)

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications