COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 23-MAY-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_pUSA030019 [new locus tag: SAUSA300_RS14830 ]
• pan locus tag^?:
• symbol: traJ
• pan gene symbol^?: —
• synonym:
• product: transfer protein complex TraJ

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_pUSA030019 [new locus tag: SAUSA300_RS14830 ]
• symbol: traJ
• product: transfer protein complex TraJ
• replicon: pUSA03
• strand: +
• coordinates: 19532..19996
• length: 465
• essential: unknown

⊟Accession numbers

• Gene ID: 3912755 NCBI
• RefSeq: YP_492705 NCBI
• BioCyc: see SAUSA300_RS14830
• MicrobesOnline: 1291498 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
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  421
  
  GTGAAAAAAGGAATATTCATTTTAACTGGTATTATTTGGCTAGTATTAATATATTTAGCG
  CAAATATTAGCATATGCTTTAAATACACTAAGTAATACTAGCAATAAGCAAAAGATATTT
  AGTGATATATCACAGTTGTTTATACCTCCAGCTAAACACCCAATAAGCACTATTATCGGT
  TTTGTATCTGATAAAAATACAGTGTTTTTTGTAATAGGTGTAATAGCAACAATTATTTGT
  ATATATATTCTTATAAAACAATTATTTGAGAGCAAAGACGGAAACGAAAAAAATCAAGAC
  TATAAAATTGCTAAACATGGTTCTCATGGATCAGCTAAATTTGCCGAAGATAGCGAATTG
  TTTTCAGAAGGACATTATAAAAAAATGAAAGAAAACGACATTACGAACTATGTTTATAAC
  AGTTTAGATAAATCATTAATAGAAGAAAGAAGTGATAAGGAATGA
  60
  120
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  420
  465
  

⊟Protein

⊟General

• locus tag: SAUSA300_pUSA030019 [new locus tag: SAUSA300_RS14830 ]
• symbol: TraJ
• description: transfer protein complex TraJ
• length: 154
• theoretical pI: 8.40965
• theoretical MW: 17469.1
• GRAVY: -0.0435065

⊟Function

• TIGRFAM:
  Unknown function General heme biosynthesis-associated TPR protein (TIGR00540; HMM-score: 12.1)
  integral membrane protein (TIGR04561; HMM-score: 11.7)
• TheSEED  :
  • putative membrane protein TraJ
• PFAM:
  no clan defined CrgA; Cell division protein CrgA (PF06781; HMM-score: 12.2)
  DUF4834; Domain of unknown function (DUF4834) (PF16118; HMM-score: 11.4)
  Tetraspannin (CL0347) DUF4064; Protein of unknown function (DUF4064) (PF13273; HMM-score: 11.3)
  no clan defined SPC12; Microsomal signal peptidase 12 kDa subunit (SPC12) (PF06645; HMM-score: 11.1)
  Rab5ip; Rab5-interacting protein (Rab5ip) (PF07019; HMM-score: 10.6)
  and 1 more
  LMBR1; LMBR1-like membrane protein (PF04791; HMM-score: 9.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.32
  • Cytoplasmic Membrane Score: 9.55
  • Cellwall Score: 0.12
  • Extracellular Score: 0.01
  • Internal Helices: 2
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: Signal peptide SP(Sec/SPI) length 26 aa
  • SP(Sec/SPI): 0.750432
  • TAT(Tat/SPI): 0.013522
  • LIPO(Sec/SPII): 0.019447
  • Cleavage Site: CS pos: 26-27. AYA-LN. Pr: 0.6184
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI: 87159852 NCBI
• RefSeq: YP_492705 NCBI
• UniProt: A0A0H2XJ91 UniProt

⊟Protein sequence

• MKKGIFILTGIIWLVLIYLAQILAYALNTLSNTSNKQKIFSDISQLFIPPAKHPISTIIGFVSDKNTVFFVIGVIATIICIYILIKQLFESKDGNEKNQDYKIAKHGSHGSAKFAEDSELFSEGHYKKMKENDITNYVYNSLDKSLIEERSDKE

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: traA > traB > traC > traD > traE > traF > traG > traH > traI > traJ > traK

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications