COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 23-MAY-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_pUSA030017 [new locus tag: SAUSA300_RS14820 ]
• pan locus tag^?:
• symbol: traH
• pan gene symbol^?: —
• synonym:
• product: transfer complex protein TraH

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_pUSA030017 [new locus tag: SAUSA300_RS14820 ]
• symbol: traH
• product: transfer complex protein TraH
• replicon: pUSA03
• strand: +
• coordinates: 16913..17398
• length: 486
• essential: unknown

⊟Accession numbers

• Gene ID: 3912759 NCBI
• RefSeq: YP_492703 NCBI
• BioCyc: see SAUSA300_RS14820
• MicrobesOnline: 1291496 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAAAAATTATTAACTATAGTTATTTTATTCACTTTGGTTCTGGCTGGTTGTGGCCAA
  AATCAAAGTGAGAAAAATAGTGAAAAACATAATGAAGAAAAAACAGTAGATACTACAGAA
  GAAAGAAACGATATGATAAAAGGTCTAATTGAATATGGTTATGAAAATGATGATTATAAA
  GGTTATAAGCATATGAAAGATATATTATCTGAAAAGTTGCAAAAGCAAGCAGAATTGCAA
  AATCAATCAGCACAAACTAAAGGTGTAAAAAAAGAAACTAAAGACATTAAACTTTATCAA
  GACGTAGATAATGATGATGAATTGATTTATTCTATTAAACTTTCAATTACAAACAAAAAA
  GAGAAAAACTCTGACTACTCAATGAGATATGGGAAAATTGAATTAACGGAAGAAGAAGGT
  AAACAAAAGATAGATAATGTCGAAGAACTAACACAAAGAAATTTAGTAAGTATGAATCAA
  GAATAG
  60
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  486
  

⊟Protein

⊟General

• locus tag: SAUSA300_pUSA030017 [new locus tag: SAUSA300_RS14820 ]
• symbol: TraH
• description: transfer complex protein TraH
• length: 161
• theoretical pI: 4.87837
• theoretical MW: 18763
• GRAVY: -1.0795

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Sporulation and germination sporulation lipoprotein, YhcN/YlaJ family (TIGR02898; HMM-score: 17)
  Cell structure Cell envelope Other uncharacterized lipoprotein (TIGR02722; HMM-score: 15.6)
  and 1 more
  extracellular lipase, Pla-1/cef family (TIGR03502; EC 3.1.-.-; HMM-score: 11.3)
• TheSEED  :
  • FIG01110541: hypothetical protein
• PFAM:
  LppaM (CL0421) LPAM_2; Prokaryotic lipoprotein-attachment site (PF13627; HMM-score: 12.6)
  and 1 more
  FtsL (CL0225) DivIC; Septum formation initiator (PF04977; HMM-score: 7.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helices: 0
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: 2
  • Predicted Cleavage Site: No CleavageSite
• SignalP: Signal peptide LIPO(Sec/SPII) length 17 aa
  • SP(Sec/SPI): 0.000372
  • TAT(Tat/SPI): 0.000083
  • LIPO(Sec/SPII): 0.99931
  • Cleavage Site: CS pos: 17-18. LAG-CG. Pr: 0.9999
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87159856 NCBI
• RefSeq: YP_492703 NCBI
• UniProt: A0A0H2XKN3 UniProt

⊟Protein sequence

• MKKLLTIVILFTLVLAGCGQNQSEKNSEKHNEEKTVDTTEERNDMIKGLIEYGYENDDYKGYKHMKDILSEKLQKQAELQNQSAQTKGVKKETKDIKLYQDVDNDDELIYSIKLSITNKKEKNSDYSMRYGKIELTEEEGKQKIDNVEELTQRNLVSMNQE

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: traA > traB > traC > traD > traE > traF > traG > traH > traI > traJ > traK

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications