COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 23-MAY-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_pUSA030011 [new locus tag: SAUSA300_RS14790 ]
• pan locus tag^?:
• symbol: traB
• pan gene symbol^?: —
• synonym:
• product: transfer complex protein TraB

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_pUSA030011 [new locus tag: SAUSA300_RS14790 ]
• symbol: traB
• product: transfer complex protein TraB
• replicon: pUSA03
• strand: +
• coordinates: 11030..11347
• length: 318
• essential: unknown

⊟Accession numbers

• Gene ID: 3912742 NCBI
• RefSeq: YP_492697 NCBI
• BioCyc: see SAUSA300_RS14790
• MicrobesOnline: 1291490 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  
  ATGATTAAAAAATTCAGTTTAACAACTGTTTATGTAGCATTTTTAAGCATTGTTTTATCA
  AATATAACACTTGGCGCAGAAAATCCAGGGCCGAAAATTGAACAAGGTTTACAACAAGTA
  CAAACATTCTTAACAGGCCTAATTGTTGCTGTTGGTATCTGTGCTGGTGTTTGGATAGTT
  CTTAAAAAATTACCTGGAATTGATGATCCAATGGTAAAAAATGAAATGTTTAGAGGCGTT
  GGAATGGTATTAGCTGGTGTGGCTGTTGGGGCAGCACTTGTTTGGTTGGTTCCATGGGTA
  TACAACCTTTTCCAATAA
  60
  120
  180
  240
  300
  318
  

⊟Protein

⊟General

• locus tag: SAUSA300_pUSA030011 [new locus tag: SAUSA300_RS14790 ]
• symbol: TraB
• description: transfer complex protein TraB
• length: 105
• theoretical pI: 9.19725
• theoretical MW: 11317.6
• GRAVY: 0.862857

⊟Function

• TIGRFAM:
• TheSEED  :
  • protein TrsB
• PFAM:
  no clan defined T4SS_CagC; Cag pathogenicity island, type IV secretory system (PF16943; HMM-score: 62.8)
  and 5 more
  TrbC; TrbC/VIRB2 family (PF04956; HMM-score: 23.6)
  DUF2157; Predicted membrane protein (DUF2157) (PF09925; HMM-score: 21.1)
  Maff2; Maff2 family (PF12750; HMM-score: 14.2)
  SecE; SecE/Sec61-gamma subunits of protein translocation complex (PF00584; HMM-score: 10.4)
  O-anti_assembly (CL0499) Wzy_C; O-Antigen ligase (PF04932; HMM-score: 8.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 3
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: Signal peptide SP(Sec/SPI) length 25 aa
  • SP(Sec/SPI): 0.696851
  • TAT(Tat/SPI): 0.001428
  • LIPO(Sec/SPII): 0.076762
  • Cleavage Site: CS pos: 25-26. TLG-AE. Pr: 0.4498
• predicted transmembrane helices (TMHMM): 3

⊟Accession numbers

• GI: 87159875 NCBI
• RefSeq: YP_492697 NCBI
• UniProt: A0A0H2XKQ8 UniProt

⊟Protein sequence

• MIKKFSLTTVYVAFLSIVLSNITLGAENPGPKIEQGLQQVQTFLTGLIVAVGICAGVWIVLKKLPGIDDPMVKNEMFRGVGMVLAGVAVGAALVWLVPWVYNLFQ

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: traA > traB > traC > traD > traE > traF > traG > traH > traI > traJ > traK

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications