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COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 23-MAY-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_pUSA030015 [new locus tag: SAUSA300_RS14810 ]
  • pan locus tag?:
  • symbol: traF
  • pan gene symbol?: —
  • synonym:
  • product: transfer complex protein TraF

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_pUSA030015 [new locus tag: SAUSA300_RS14810 ]
  • symbol: traF
  • product: transfer complex protein TraF
  • replicon: pUSA03
  • strand: +
  • coordinates: 14529..15809
  • length: 1281
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAAAATTAGAAATTTTAATGAAGGAACATTGGTAGGAAAACATAACCACTTAGAATAT
    ATCATCAATGTTGATTTGTGCCATATATCAGATATAGACGAAATTAGATATGACTTAACA
    GAACCTAATGAATTGTTTTTTGAAGTTAAAGATTTACAAAAGGAAGGCGATTATTTTCAT
    ATCATTTACAACACTGATAATGAATATAATTCCTTCCTATCAGCAAAAAAAGAAAGCAAA
    GCACTCAAATTATCTTTAATGGATTATGTGTTAAAAGTTGATCCATTAAAGGACAATATA
    ACTGTTATGCACCCAGCGAACCTATTTTTTAAAAATATTGAGGATATAAAAATAGGATTC
    AAAGGCCATGAATATTTACCTAAACCTAAAATAAATAATTTAGAACAGTATAAATTGTTA
    ATAATGAGTACATTATCACAATACACGTTTGATAAGTATTATCGCAATAAATTTGAAGTT
    TTATCAAAAGAAAAAGATGATTTTTTCCATAAAGTAAATAATGCAGAAACTTTTGAAAAA
    TTGAAAGAGATTGTAAGACATGAATTGAATCAAGTACAGACAGATTATTTACTGAATGAA
    GAAAAACGTAAAAGAGAAACACGAAAAAAGTACATTAGAAATTTAATACTTGGTTTTGTT
    GGTATTGCAATAGTTATAAGTTTAATTTCATTTATGATTATAAACGGAAAGCAACAAGAA
    TTAGATAGCAAAATAGCACAAGCAGACAAACAAGAACAAAGAAGTAAAGTATATGAAAAT
    CTTTACAATGGCAATGTTGATCAAGCAGTTAAAGGCATGAAACGTGACGATAGCTTTAAC
    AAAAAAGATATTGAAAAAACATTAAAAAAAGAAAAGAAATATGAAGAACTAATTGAATTG
    AGTAGTAAAAACACACCTTATGTAATTGAACAATTATATAAAGAAGGTAAGCAGAATAAA
    ATAAGAGAGTTAGCTTTCAATTTTGAAAATAACGATACATTGAGTTTAGAAAAGAAAATA
    TTAGACAAAGACGGAACAGCATTTAGTGTAGGCTCAACAGGTAAATACGAAGAACAAAGT
    AAACGTTTGGCTTTAGCGTCAGCTAAAGAAGGTTATGTTGAATCAGCTAAAGATATTAAT
    AAAAAACTTAAAGATAATGAAGTTGAAGAAGAAATAAATAAAGCTGAAATTAAACAACTA
    AAAGAAGAAAAAGACAGCACGAAGGATAAAGACAAGAAAAAAGAAATCCAAAAAGATATA
    GAGGAATTAGAGAAGAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1281

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_pUSA030015 [new locus tag: SAUSA300_RS14810 ]
  • symbol: TraF
  • description: transfer complex protein TraF
  • length: 426
  • theoretical pI: 7.29157
  • theoretical MW: 50186.8
  • GRAVY: -0.865023

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • TraF
  • PFAM:
    no clan defined T2SSF; Type II secretion system (T2SS), protein F (PF00482; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • LocateP: Intracellular /TMH start AFTER 60
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: Possibly Sec-
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002448
    • TAT(Tat/SPI): 0.000177
    • LIPO(Sec/SPII): 0.000784
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKIRNFNEGTLVGKHNHLEYIINVDLCHISDIDEIRYDLTEPNELFFEVKDLQKEGDYFHIIYNTDNEYNSFLSAKKESKALKLSLMDYVLKVDPLKDNITVMHPANLFFKNIEDIKIGFKGHEYLPKPKINNLEQYKLLIMSTLSQYTFDKYYRNKFEVLSKEKDDFFHKVNNAETFEKLKEIVRHELNQVQTDYLLNEEKRKRETRKKYIRNLILGFVGIAIVISLISFMIINGKQQELDSKIAQADKQEQRSKVYENLYNGNVDQAVKGMKRDDSFNKKDIEKTLKKEKKYEELIELSSKNTPYVIEQLYKEGKQNKIRELAFNFENNDTLSLEKKILDKDGTAFSVGSTGKYEEQSKRLALASAKEGYVESAKDINKKLKDNEVEEEINKAEIKQLKEEKDSTKDKDKKKEIQKDIEELEKK

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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