COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 23-MAY-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_pUSA030014 [new locus tag: SAUSA300_RS14805 ]
• pan locus tag^?:
• symbol: traE
• pan gene symbol^?: —
• synonym:
• product: transfer complex protein TraE

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_pUSA030014 [new locus tag: SAUSA300_RS14805 ]
• symbol: traE
• product: transfer complex protein TraE
• replicon: pUSA03
• strand: +
• coordinates: 12505..14517
• length: 2013
• essential: unknown

⊟Accession numbers

• Gene ID: 3912751 NCBI
• RefSeq: YP_492700 NCBI
• BioCyc: see SAUSA300_RS14805
• MicrobesOnline: 1291493 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGCATTTTTAAAAAAGAAAAAGCAAGAGCAAGTTACAAATAAAATTCACAATGAATCT
  TTTCAAAAATTAACAGAAGTATTAGATACAGATTCTGTTGATAGTATTTATCCTTTTAGT
  TGGATAGAGAAAAAATCGCATATAGAAACAGGCGAAAATTATATTAAAAATTTATTAGTA
  GTTGACTACCCTCAATCAGTAAAAGGGGCGTACTTATCAAATTTATTAAAGAAAAACGGT
  AATATTCAAATAACTAAATTTATTAGACCAGCAAATATAGAAAGAATGATTGACCATTTA
  AACAACTCAATCAAAAACAAAACAGCAGAACAAATGCGTACTACAGACCCTAAACGAAAT
  GCAACAATTAAAAGAGAAATTGAAAGTAGTAAAAAACAACTTGATAAATTTTTAGATGAA
  AAAACAGGATTTATGTACATGTATATGTATATCACGTTAAATGGCGATTCATACGAAAAG
  ATACAAGCGCTAGAAAAAGATGTTAAGCGTACTTTAACGAGATTAAGACTTAAAACACAT
  ACACCTACAAACGCAATGCGAGAATCATTTCATACAGTTCTTCCTTTAAATAGAAACTTC
  TTATCAGCATTTACACAACAAAATATGGATACAGCAACAGCAGGGCATTTCTTTATGTTT
  GATGATTCAGAAATTATTGATTTAACACCTAATACATCAGTTTTTGGAATTAACAAAAAT
  ACAGATTCATTAGTAGCAGTTGATTTTAATAACAAAGAAAAAACTTTAAATAAAAATATG
  ACTATTATCGGTACAAGTGGTGTAGGTAAATCTACCCTCAATATGAGAATGATTTTAGAC
  AATATCAAAAAATCTATAAGACAATTTATCATTGATCCCGAAGATGAATTTTCATATATA
  ACTAAATATTACGGTGGAACAGTTGTAAATATTTCAACAAGTTCAAATATCAAAATTAAT
  CCATTTGAAATTTTTTCAGAAGAAGTATTTGAAAAAGAAGATGAAACAGACAATGAAACA
  ACATCAGATGTTTTAACGGAAAACAACGAACACGAAAGCCAAATTGACACATTAGTTCGT
  TCTAAAATAGGGAAGTTAAAAACCTTCTTTAGAGTTCTTAAAGATGAAATATCTCAAACA
  GAAATATCTGTATTATCTTCAACTTTAAGACAGTTATATCAAGATAAAGGATTTAAAGGT
  AATGCGAAATTATCAGATTTCAAAAGCGAAGATTATCCAACTTTAACAGAATTATATAAC
  AAACTAAAAGACTTAGACCCAGAAAAATATGAGGTATTAAAGGACTTAACACTTATCATA
  GAAGATTACACAATGGAACATGGTACTACTACAATCTTTGACGGTTATACAAACATCAAA
  TTAGATAATGAAATTGTTACTTTTAACCTTAAACCATTACAAACAGAAAAAGATGTTCAA
  AGTGCAGCGTATTTGAATATATTCAGTTTCTTATGGGACGAAATAACAAAAGATAGAACA
  ACAGAAACAGTATTAATGACTGACGAATTACATTTCTTAGCAACAAATGAATATAGCTTA
  GATTTTTATTATCAAGCATACAAGCGTATTAGAAAATATGGCGGTGGCGCTATTGCCTCT
  ACTCAACAAATTAAAGATATTCTTAGAACATCACAAGAAATAGGTTCAGCTATTATTGAA
  AATAGTCATACTAAATTCTTCTTTGGAATGGATAACGTAGGCGTTGATGATGTTGTTGAT
  AAATTAGGATTGAAATTTAGCGATCAAGAAATATCTCATTTAACTAAAAAGAAAAAAGGC
  GAGGCATTATTACTATACGGAACACAAAGAGCATTTATAAGAATTGATTTAGATAGAGAA
  GAAACAAGACTTTGGAATAAAGAATTATATGAAACAATTTATGAAGAACCAGCAGACGTA
  GAGCCGAATTATGTTGAACAACTAGGATTAACAGATATTGATTTAGCAGAGTTGGAAGAA
  GAATTAAGACAAGCAGAAATGATTTATGAATAG
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⊟Protein

⊟General

• locus tag: SAUSA300_pUSA030014 [new locus tag: SAUSA300_RS14805 ]
• symbol: TraE
• description: transfer complex protein TraE
• length: 670
• theoretical pI: 4.91695
• theoretical MW: 77594.2
• GRAVY: -0.568358

⊟Function

• TIGRFAM:
  type-IV secretion system protein TraC (TIGR02746; HMM-score: 116.9)
  type IV secretion/conjugal transfer ATPase, VirB4 family (TIGR00929; HMM-score: 100.4)
  and 3 more
  Genetic information processing Mobile and extrachromosomal element functions Plasmid functions conjugative transfer ATPase, PFL_4706 family (TIGR03744; HMM-score: 27.2)
  Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate synthase (TIGR00033; EC 4.2.3.5; HMM-score: 13.9)
  Metabolism Transport and binding proteins Amino acids, peptides and amines glycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 13.4)
• TheSEED  :
  • putative ATPase TraE
• PFAM:
  P-loop_NTPase (CL0023) DUF87; Domain of unknown function DUF87 (PF01935; HMM-score: 36.3)
  AAA_10; AAA-like domain (PF12846; HMM-score: 30.5)
  and 11 more
  TrwB_AAD_bind; Type IV secretion-system coupling protein DNA-binding domain (PF10412; HMM-score: 22.2)
  AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 15.5)
  TraG-D_C; TraM recognition site of TraD and TraG (PF12696; HMM-score: 15.1)
  no clan defined Maf1; Maf1 regulator (PF09174; HMM-score: 13.2)
  P-loop_NTPase (CL0023) AAA_16; AAA ATPase domain (PF13191; HMM-score: 13.1)
  ABC_tran; ABC transporter (PF00005; HMM-score: 12.9)
  RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 12.9)
  IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 12.7)
  AAA_22; AAA domain (PF13401; HMM-score: 12.6)
  FtsK_SpoIIIE; FtsK/SpoIIIE family (PF01580; HMM-score: 12)
  no clan defined Mlh1_C; DNA mismatch repair protein Mlh1 C-terminus (PF16413; HMM-score: 11.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003011
  • TAT(Tat/SPI): 0.000217
  • LIPO(Sec/SPII): 0.000401
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87159848 NCBI
• RefSeq: YP_492700 NCBI
• UniProt: A0A0H2XKN8 UniProt

⊟Protein sequence

• MAFLKKKKQEQVTNKIHNESFQKLTEVLDTDSVDSIYPFSWIEKKSHIETGENYIKNLLVVDYPQSVKGAYLSNLLKKNGNIQITKFIRPANIERMIDHLNNSIKNKTAEQMRTTDPKRNATIKREIESSKKQLDKFLDEKTGFMYMYMYITLNGDSYEKIQALEKDVKRTLTRLRLKTHTPTNAMRESFHTVLPLNRNFLSAFTQQNMDTATAGHFFMFDDSEIIDLTPNTSVFGINKNTDSLVAVDFNNKEKTLNKNMTIIGTSGVGKSTLNMRMILDNIKKSIRQFIIDPEDEFSYITKYYGGTVVNISTSSNIKINPFEIFSEEVFEKEDETDNETTSDVLTENNEHESQIDTLVRSKIGKLKTFFRVLKDEISQTEISVLSSTLRQLYQDKGFKGNAKLSDFKSEDYPTLTELYNKLKDLDPEKYEVLKDLTLIIEDYTMEHGTTTIFDGYTNIKLDNEIVTFNLKPLQTEKDVQSAAYLNIFSFLWDEITKDRTTETVLMTDELHFLATNEYSLDFYYQAYKRIRKYGGGAIASTQQIKDILRTSQEIGSAIIENSHTKFFFGMDNVGVDDVVDKLGLKFSDQEISHLTKKKKGEALLLYGTQRAFIRIDLDREETRLWNKELYETIYEEPADVEPNYVEQLGLTDIDLAELEEELRQAEMIYE

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: traA > traB > traC > traD > traE > traF > traG > traH > traI > traJ > traK

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications