Jump to navigation
Jump to search
COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40
NCBI: 23-MAY-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_pUSA030014 [new locus tag: SAUSA300_RS14805 ]
- pan locus tag?:
- symbol: traE
- pan gene symbol?: —
- synonym:
- product: transfer complex protein TraE
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_pUSA030014 [new locus tag: SAUSA300_RS14805 ]
- symbol: traE
- product: transfer complex protein TraE
- replicon: pUSA03
- strand: +
- coordinates: 12505..14517
- length: 2013
- essential: unknown
⊟Accession numbers[edit | edit source]
- Gene ID: 3912751 NCBI
- RefSeq: YP_492700 NCBI
- BioCyc: see SAUSA300_RS14805
- MicrobesOnline: 1291493 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981ATGGCATTTTTAAAAAAGAAAAAGCAAGAGCAAGTTACAAATAAAATTCACAATGAATCT
TTTCAAAAATTAACAGAAGTATTAGATACAGATTCTGTTGATAGTATTTATCCTTTTAGT
TGGATAGAGAAAAAATCGCATATAGAAACAGGCGAAAATTATATTAAAAATTTATTAGTA
GTTGACTACCCTCAATCAGTAAAAGGGGCGTACTTATCAAATTTATTAAAGAAAAACGGT
AATATTCAAATAACTAAATTTATTAGACCAGCAAATATAGAAAGAATGATTGACCATTTA
AACAACTCAATCAAAAACAAAACAGCAGAACAAATGCGTACTACAGACCCTAAACGAAAT
GCAACAATTAAAAGAGAAATTGAAAGTAGTAAAAAACAACTTGATAAATTTTTAGATGAA
AAAACAGGATTTATGTACATGTATATGTATATCACGTTAAATGGCGATTCATACGAAAAG
ATACAAGCGCTAGAAAAAGATGTTAAGCGTACTTTAACGAGATTAAGACTTAAAACACAT
ACACCTACAAACGCAATGCGAGAATCATTTCATACAGTTCTTCCTTTAAATAGAAACTTC
TTATCAGCATTTACACAACAAAATATGGATACAGCAACAGCAGGGCATTTCTTTATGTTT
GATGATTCAGAAATTATTGATTTAACACCTAATACATCAGTTTTTGGAATTAACAAAAAT
ACAGATTCATTAGTAGCAGTTGATTTTAATAACAAAGAAAAAACTTTAAATAAAAATATG
ACTATTATCGGTACAAGTGGTGTAGGTAAATCTACCCTCAATATGAGAATGATTTTAGAC
AATATCAAAAAATCTATAAGACAATTTATCATTGATCCCGAAGATGAATTTTCATATATA
ACTAAATATTACGGTGGAACAGTTGTAAATATTTCAACAAGTTCAAATATCAAAATTAAT
CCATTTGAAATTTTTTCAGAAGAAGTATTTGAAAAAGAAGATGAAACAGACAATGAAACA
ACATCAGATGTTTTAACGGAAAACAACGAACACGAAAGCCAAATTGACACATTAGTTCGT
TCTAAAATAGGGAAGTTAAAAACCTTCTTTAGAGTTCTTAAAGATGAAATATCTCAAACA
GAAATATCTGTATTATCTTCAACTTTAAGACAGTTATATCAAGATAAAGGATTTAAAGGT
AATGCGAAATTATCAGATTTCAAAAGCGAAGATTATCCAACTTTAACAGAATTATATAAC
AAACTAAAAGACTTAGACCCAGAAAAATATGAGGTATTAAAGGACTTAACACTTATCATA
GAAGATTACACAATGGAACATGGTACTACTACAATCTTTGACGGTTATACAAACATCAAA
TTAGATAATGAAATTGTTACTTTTAACCTTAAACCATTACAAACAGAAAAAGATGTTCAA
AGTGCAGCGTATTTGAATATATTCAGTTTCTTATGGGACGAAATAACAAAAGATAGAACA
ACAGAAACAGTATTAATGACTGACGAATTACATTTCTTAGCAACAAATGAATATAGCTTA
GATTTTTATTATCAAGCATACAAGCGTATTAGAAAATATGGCGGTGGCGCTATTGCCTCT
ACTCAACAAATTAAAGATATTCTTAGAACATCACAAGAAATAGGTTCAGCTATTATTGAA
AATAGTCATACTAAATTCTTCTTTGGAATGGATAACGTAGGCGTTGATGATGTTGTTGAT
AAATTAGGATTGAAATTTAGCGATCAAGAAATATCTCATTTAACTAAAAAGAAAAAAGGC
GAGGCATTATTACTATACGGAACACAAAGAGCATTTATAAGAATTGATTTAGATAGAGAA
GAAACAAGACTTTGGAATAAAGAATTATATGAAACAATTTATGAAGAACCAGCAGACGTA
GAGCCGAATTATGTTGAACAACTAGGATTAACAGATATTGATTTAGCAGAGTTGGAAGAA
GAATTAAGACAAGCAGAAATGATTTATGAATAG60
120
180
240
300
360
420
480
540
600
660
720
780
840
900
960
1020
1080
1140
1200
1260
1320
1380
1440
1500
1560
1620
1680
1740
1800
1860
1920
1980
2013
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_pUSA030014 [new locus tag: SAUSA300_RS14805 ]
- symbol: TraE
- description: transfer complex protein TraE
- length: 670
- theoretical pI: 4.91695
- theoretical MW: 77594.2
- GRAVY: -0.568358
⊟Function[edit | edit source]
- TIGRFAM: type-IV secretion system protein TraC (TIGR02746; HMM-score: 116.9)type IV secretion/conjugal transfer ATPase, VirB4 family (TIGR00929; HMM-score: 100.4)and 3 moreMobile and extrachromosomal element functions Plasmid functions conjugative transfer ATPase, PFL_4706 family (TIGR03744; HMM-score: 27.2)Amino acid biosynthesis Aromatic amino acid family chorismate synthase (TIGR00033; EC 4.2.3.5; HMM-score: 13.9)Transport and binding proteins Amino acids, peptides and amines glycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 13.4)
- TheSEED :
- putative ATPase TraE
- PFAM: P-loop_NTPase (CL0023) DUF87; Domain of unknown function DUF87 (PF01935; HMM-score: 36.3)AAA_10; AAA-like domain (PF12846; HMM-score: 30.5)and 11 moreTrwB_AAD_bind; Type IV secretion-system coupling protein DNA-binding domain (PF10412; HMM-score: 22.2)AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 15.5)TraG-D_C; TraM recognition site of TraD and TraG (PF12696; HMM-score: 15.1)no clan defined Maf1; Maf1 regulator (PF09174; HMM-score: 13.2)P-loop_NTPase (CL0023) AAA_16; AAA ATPase domain (PF13191; HMM-score: 13.1)ABC_tran; ABC transporter (PF00005; HMM-score: 12.9)RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 12.9)IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 12.7)AAA_22; AAA domain (PF13401; HMM-score: 12.6)FtsK_SpoIIIE; FtsK/SpoIIIE family (PF01580; HMM-score: 12)no clan defined Mlh1_C; DNA mismatch repair protein Mlh1 C-terminus (PF16413; HMM-score: 11.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.003011
- TAT(Tat/SPI): 0.000217
- LIPO(Sec/SPII): 0.000401
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MAFLKKKKQEQVTNKIHNESFQKLTEVLDTDSVDSIYPFSWIEKKSHIETGENYIKNLLVVDYPQSVKGAYLSNLLKKNGNIQITKFIRPANIERMIDHLNNSIKNKTAEQMRTTDPKRNATIKREIESSKKQLDKFLDEKTGFMYMYMYITLNGDSYEKIQALEKDVKRTLTRLRLKTHTPTNAMRESFHTVLPLNRNFLSAFTQQNMDTATAGHFFMFDDSEIIDLTPNTSVFGINKNTDSLVAVDFNNKEKTLNKNMTIIGTSGVGKSTLNMRMILDNIKKSIRQFIIDPEDEFSYITKYYGGTVVNISTSSNIKINPFEIFSEEVFEKEDETDNETTSDVLTENNEHESQIDTLVRSKIGKLKTFFRVLKDEISQTEISVLSSTLRQLYQDKGFKGNAKLSDFKSEDYPTLTELYNKLKDLDPEKYEVLKDLTLIIEDYTMEHGTTTIFDGYTNIKLDNEIVTFNLKPLQTEKDVQSAAYLNIFSFLWDEITKDRTTETVLMTDELHFLATNEYSLDFYYQAYKRIRKYGGGAIASTQQIKDILRTSQEIGSAIIENSHTKFFFGMDNVGVDDVVDKLGLKFSDQEISHLTKKKKGEALLLYGTQRAFIRIDLDREETRLWNKELYETIYEEPADVEPNYVEQLGLTDIDLAELEEELRQAEMIYE
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: no data available
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.