COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 23-MAY-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_pUSA030010 [new locus tag: SAUSA300_RS14785 ]
• pan locus tag^?:
• symbol: traA
• pan gene symbol^?: —
• synonym:
• product: transfer complex protein TraA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_pUSA030010 [new locus tag: SAUSA300_RS14785 ]
• symbol: traA
• product: transfer complex protein TraA
• replicon: pUSA03
• strand: +
• coordinates: 10039..11013
• length: 975
• essential: unknown

⊟Accession numbers

• Gene ID: 3912735 NCBI
• RefSeq: YP_492696 NCBI
• BioCyc: see SAUSA300_RS14785
• MicrobesOnline: 1291489 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  TTGGATAACATAACATTTCGTAAACGTATTGAAGAATATCGAAAGCGATCTATTGAGCAA
  AAGAAATATGAGAGCAAACTAAATTATCAATCAAGATTTGGTGTGAAAGATGATGAAGTT
  AGTACTTTTAATATCAAAGGCATTGCTACAGATAAAAAAGAAAATAAGTTAGTCATGGCC
  GAAGATAGAGAGGATAAACAAAAAGTTCTTTTATACGCCAATAAAGATAATGATATGTAT
  TATTTGAATTCTGGTAGCACTTATAAAGAGGCTGTTCAAAACACTTTTGGTAATGAAACC
  AATTACGAAATGGTAGATGATGAATTATTTATTTATAATTCAAAAAATCAAGCACTTACA
  AACTATGACCAACTTGATGAGTATCACAATCGACAAAAGCAATTACAATATAACACCAAA
  TTAGAACAGGAACGAAAGCAAAGATTGACTGATACAAATAAAGTTAGTGAAAATGCTAAA
  GAAGATTTAGCGTTAAGTTTAGGACTTACAGCAACAGCATTGAAAGGTAACATAAACATT
  TCTAATGTTGAAAAAGTCACTGATAATGAAAGTATGGTTACTATAGAAAATGAAATTAAT
  GGTAAGTCAGAACATTTAATTTCAGACTTTAAAGATAGTTTCATTGAAAAGGCTAAAGAA
  AAGCTAACTGTTTCCCCTTCAATGAGGTTAGGATTTGAAGAATTTAAAAACGCCTCGGGA
  CAAATAGCAATGGGATTTACAGAGTTTTTTGGCGCTAGGTATGACGAAAGCACTTTAGAA
  AACGACCCTAAAGTTGTGGCAACAGAAAAAGGGTACTCTGTTTCTGTTTTTGATAAAGAC
  GGAAACAAAAAAGATTTAACATCAGCTGACACTTTTGAGGAGGCAGTAAAATTATCCAAA
  GAAATGAAACATGATAACTCGCTTTCTCGTAGTGAAATAGAATTAAATCAAGAATTAGAA
  AAGAAAAAAATATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_pUSA030010 [new locus tag: SAUSA300_RS14785 ]
• symbol: TraA
• description: transfer complex protein TraA
• length: 324
• theoretical pI: 4.91477
• theoretical MW: 37481.4
• GRAVY: -0.988889

⊟Function

• TIGRFAM:
  Genetic information processing Mobile and extrachromosomal element functions Plasmid functions integrating conjugative element protein, PFL_4705 family (TIGR03752; HMM-score: 11.8)
• TheSEED  :
  • Transfer complex protein TraA
• PFAM:
  Phage_barrel (CL0504) Phage-tail_2; Baseplate structural protein, domain 2 (PF09096; HMM-score: 14.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004808
  • TAT(Tat/SPI): 0.000436
  • LIPO(Sec/SPII): 0.001008
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87159868 NCBI
• RefSeq: YP_492696 NCBI
• UniProt: A0A0H2XJB3 UniProt

⊟Protein sequence

• MDNITFRKRIEEYRKRSIEQKKYESKLNYQSRFGVKDDEVSTFNIKGIATDKKENKLVMAEDREDKQKVLLYANKDNDMYYLNSGSTYKEAVQNTFGNETNYEMVDDELFIYNSKNQALTNYDQLDEYHNRQKQLQYNTKLEQERKQRLTDTNKVSENAKEDLALSLGLTATALKGNINISNVEKVTDNESMVTIENEINGKSEHLISDFKDSFIEKAKEKLTVSPSMRLGFEEFKNASGQIAMGFTEFFGARYDESTLENDPKVVATEKGYSVSVFDKDGNKKDLTSADTFEEAVKLSKEMKHDNSLSRSEIELNQELEKKKI

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: traA > traB > traC > traD > traE > traF > traG > traH > traI > traJ > traK

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications