COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 23-MAY-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_pUSA030012 [new locus tag: SAUSA300_RS14795 ]
• pan locus tag^?:
• symbol: traC
• pan gene symbol^?: —
• synonym:
• product: membrane protein TraC

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_pUSA030012 [new locus tag: SAUSA300_RS14795 ]
• symbol: traC
• product: membrane protein TraC
• replicon: pUSA03
• strand: +
• coordinates: 11407..11814
• length: 408
• essential: unknown

⊟Accession numbers

• Gene ID: 3912752 NCBI
• RefSeq: YP_492698 NCBI
• BioCyc: see SAUSA300_RS14795
• MicrobesOnline: 1291491 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  
  ATGTCTGTTGAGCAAGAAGTTAAAGATATAATGCTCGATATGTTGGGCGAAGAAAAGAAA
  GTTAAAGAGAATATAATACCTCGTAATGTAGACGCTAGTTATAAAATATTCTTAAATTTA
  AACGGTAAAGAATTAGCAATTGTATTTGTTCCTTTTTTACTATTTCTAACAATAGCATTT
  GGTGTAACGTTTTTAATGGGCTTACTTAATTTAATAACTGGCTTTATGGTTTTTATAGTA
  GGTTTGATTTTTGGTTTAACAATATATGGCTTACTAACAATTAGACCAATTAGTACAAAA
  GAAAATATAAGAATGATTGATACCATAAAACAATCACAACGATTTAGCAGAAGGCAGAAA
  GTTTACTTCTATAAAAGTAAAGAAGGATTGGGTGATGATGATGTTTAA
  60
  120
  180
  240
  300
  360
  408
  

⊟Protein

⊟General

• locus tag: SAUSA300_pUSA030012 [new locus tag: SAUSA300_RS14795 ]
• symbol: TraC
• description: membrane protein TraC
• length: 135
• theoretical pI: 9.41803
• theoretical MW: 15476.3
• GRAVY: 0.331852

⊟Function

• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides intracellular adhesion protein D (TIGR03932; HMM-score: 11.2)
• TheSEED  :
  • putative membrane protein TraC
• PFAM:
  no clan defined PrgI; PrgI family protein (PF12666; HMM-score: 19.1)
  and 9 more
  Phage_holin_3_6; Putative Actinobacterial Holin-X, holin superfamily III (PF07332; HMM-score: 15)
  YwcE; Spore morphogenesis and germination protein YwcE (PF17368; HMM-score: 14.3)
  DUF2207; Predicted membrane protein (DUF2207) (PF09972; HMM-score: 13.7)
  DUF2149; Uncharacterized conserved protein (DUF2149) (PF09919; HMM-score: 13)
  Myc_target_1; Myc target protein 1 (PF15179; HMM-score: 12.4)
  YuiB; Putative membrane protein (PF14068; HMM-score: 9.6)
  APC (CL0062) AA_permease_C; C-terminus of AA_permease (PF13906; HMM-score: 9.4)
  no clan defined Promethin; Promethin (PF16015; HMM-score: 7.3)
  DUF5469; Family of unknown function (DUF5469) (PF17561; HMM-score: 7.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.32
  • Cytoplasmic Membrane Score: 9.55
  • Cellwall Score: 0.12
  • Extracellular Score: 0.01
  • Internal Helices: 2
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004948
  • TAT(Tat/SPI): 0.000137
  • LIPO(Sec/SPII): 0.002723
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI: 87159849 NCBI
• RefSeq: YP_492698 NCBI
• UniProt: A0A0H2XIT4 UniProt

⊟Protein sequence

• MSVEQEVKDIMLDMLGEEKKVKENIIPRNVDASYKIFLNLNGKELAIVFVPFLLFLTIAFGVTFLMGLLNLITGFMVFIVGLIFGLTIYGLLTIRPISTKENIRMIDTIKQSQRFSRRQKVYFYKSKEGLGDDDV

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: traA > traB > traC > traD > traE > traF > traG > traH > traI > traJ > traK

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications