COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 23-MAY-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_pUSA030020 [new locus tag: SAUSA300_RS14835 ]
• pan locus tag^?:
• symbol: traK
• pan gene symbol^?: —
• synonym:
• product: transfer complex protein TraK

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_pUSA030020 [new locus tag: SAUSA300_RS14835 ]
• symbol: traK
• product: transfer complex protein TraK
• replicon: pUSA03
• strand: +
• coordinates: 19993..21633
• length: 1641
• essential: unknown

⊟Accession numbers

• Gene ID: 3912754 NCBI
• RefSeq: YP_492706 NCBI
• BioCyc: see SAUSA300_RS14835
• MicrobesOnline: 1291499 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGACTACACTTTTAGGCGAATTAGAAAGTAAAGCAACTTCTATAAATAAAAAGAATCTA
  GTAATACAAGATTTTGATACTAAATGCCCTAATAGAAATATATTTGTGGTAGGAGGGCCA
  GGTTCCTATAAGTCAGCTGGGTATGTTATTCCTAATGTTATTGTTAAAAATCAACAATCA
  ATAGTTGTAACTGACCCTAGTGGCGAAGTTTATGAAAAAACATCTAATATTAAACGCATG
  CAAGGTTTTGATGTTAGAGTAGTAAATTTCAAAAACTTTTTAGCCTCTGATAGACAAAAC
  TTTTTTGATTATATTGATAAAGATTCTGATTGTTCAATAGTTGCTGAATTAATTATTAAA
  TCAGCTGGGGATTCTAAAATAAATAAAGACGTATGGTATAAAGCCTCTGTAGGATTATTA
  AATTCATTGCTTTTATACGCTAAATATGAATTTGAACCAGAAAAACGAACTATAGGAAAC
  ATTATTAAATTTATTCAAAATAACAAACCTAATCAAGATGAAGAAGGATCAGTTGAATTA
  GATAAACGCTTTAATGAATTATCTAAAGACCACCCAGCTAGAGAAAGTTATGAATTTGGT
  TTTGCAGTATCAGAAGGAAGAACAAGAGCAAGTATTATACTAACCTTTGTAGCCGATTTA
  CGAAATTACATTGATAACGAAATTAGAAGTTATACAAGCGATTCTGATTTTGATTTACGA
  GATGTAGGATTAAGAAAAACTATTATATATGTAATGTTGCCAGTTTTGGGGAACACTGTT
  CAATCACTTTCTAGTTTATTCTTCTCTCAAATGTTCCAACAGCTATATAGGTTAGGGGAC
  GAAAACGGTGCTAGACTTCCAGTCCCAGTAGATTTTTTACTAGACGAATGGCCAAATATC
  GGCGCAATACCAGACTATGAAGAAACTTTAGCAACTTGTCGTAAGTATGGTATTAGTATC
  ACAACAATTGTTCAATCTATATCACAAGCAGTAGATAAATATAATAAAGATAAAGCTAAC
  GCTATTATAGGTAACCACGCAGTTATTTTATGTTTAGGTAATGTGAATAATGATACAGCA
  AAATATATAAAAGAAGAATTAGGTAATGCTACTGTAGAGTTTGAAACAAGTAGTGAAGGT
  TCTTCTAGTGGTAAAGATAGCAGAAGTAAATCATCAAATAAAAATGTAAGCTACACTGGA
  AGGGCATTACTAAATGAAGATGAAATAAGTAATATGCAACAGGATAAAGCTATTCTTATA
  ACTAAAAATAGAAATCCTAAAATTATTAAAAAGTTAGCATATTTTAAAATGTTCCCTAAT
  TTAACAGAAACGTATATAGCACCTCAAAACGAATACAAACGTAAAAAAAACCAGTCTATG
  ATTGATAAGCATAATAGATTAAGAGAAGAATGGGATAAAAAACAAGAGAAAAATAAACAG
  CAAAAACTAGAAGAATATAAAGAAGAACAAAGACAGAAAGAGTTAGAGAAAGAACAACAA
  CCACAAGAAGAACAACAAAATGAGAAAGTAAAAGAAAGTAAAGACAAAAACGATCAAGAA
  GATAAGAAAGACGAGCAACAAAAAATTAATGATTCTAAGAAAGCATTCTACGAAAAATTA
  AAACAAAAACAAGCTAAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_pUSA030020 [new locus tag: SAUSA300_RS14835 ]
• symbol: TraK
• description: transfer complex protein TraK
• length: 546
• theoretical pI: 7.87684
• theoretical MW: 62672.1
• GRAVY: -0.789927

⊟Function

• TIGRFAM:
  Ti-type conjugative transfer system protein TraG (TIGR02767; HMM-score: 87)
  and 2 more
  type IV conjugative transfer system coupling protein TraD (TIGR02759; HMM-score: 46.2)
  Genetic information processing Mobile and extrachromosomal element functions Other conjugative coupling factor TraD, SXT/TOL subfamily (TIGR03743; HMM-score: 42.8)
• TheSEED  :
  • protein TrsK
• PFAM:
  P-loop_NTPase (CL0023) T4SS-DNA_transf; Type IV secretory system Conjugative DNA transfer (PF02534; HMM-score: 488.3)
  and 2 more
  TraG-D_C; TraM recognition site of TraD and TraG (PF12696; HMM-score: 113.2)
  TrwB_AAD_bind; Type IV secretion-system coupling protein DNA-binding domain (PF10412; HMM-score: 72.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.03238
  • TAT(Tat/SPI): 0.001078
  • LIPO(Sec/SPII): 0.004398
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87159851 NCBI
• RefSeq: YP_492706 NCBI
• UniProt: A0A0H2XKM8 UniProt

⊟Protein sequence

• MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEVYEKTSNIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLLNSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNQDEEGSVELDKRFNELSKDHPARESYEFGFAVSEGRTRASIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGDENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVILCLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILITKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKNKQQKLEEYKEEQRQKELEKEQQPQEEQQNEKVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: traA > traB > traC > traD > traE > traF > traG > traH > traI > traJ > traK

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications