COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS14875 [old locus tag: SAUSA300_pUSA030028 ]
• pan locus tag^?:
• symbol: SAUSA300_RS14875
• pan gene symbol^?: —
• synonym:
• product: transposase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS14875 [old locus tag: SAUSA300_pUSA030028 ]
• symbol: SAUSA300_RS14875
• product: transposase
• replicon: pUSA03
• strand: +
• coordinates: 26810..27367
• length: 558
• essential: unknown

⊟Accession numbers

• Location: NC_007792 (26810..27367) NCBI
• BioCyc: SAUSA300_RS14875 BioCyc
• MicrobesOnline: see SAUSA300_pUSA030028

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGCCAATATTGGTTATGCTCGTGTATCTACCAGAGATCAAAGTTTAGATTTACAAATA
  GACGCTTTGAGTAATTTTGGTTGTGAGAAAATATTTAGCGAAAAAGTTAGTGGGCGTAAA
  GTAAATAGAACTGAACTAGATAAATGTTTAGATTATTTACGTGAAGGCGACACTTTAGTT
  ATTTACAAGCTAGATAGATTAGGCAGGACTACTAAGCAACTGATAGATTTAGCCCAATGG
  TTAGAAAATAATAATATTGAATTACAAATTATTGATATGAATATCAATACAAAAGACCCA
  ATGGGAAAGATGTTTTTTACAATGATGAGTGCTTTTGCTGAATTAGAGGCTAACTTATTA
  AGCGAAAGAACTAAAAAAGGTTTAGAATCAGCTAGAGCAAGAGGTAACATGGGAGGCAGA
  AAAGCAATGCCACAAGAAAAAAAAGAATACATTAAACATTTATACGATACCAAAGAATAT
  ACAGGACAAGAAATTGCTAAAAAAACGAATGTAAGTAGAGATACTGTATATCGCATTTTA
  CGAGAGTTTAAAAAGTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS14875 [old locus tag: SAUSA300_pUSA030028 ]
• symbol: SAUSA300_RS14875
• description: transposase
• length: 185
• theoretical pI: 9.66653
• theoretical MW: 21417.5
• GRAVY: -0.663784

⊟Function

• TIGRFAM:
  RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 20.4)
  Unknown function General DNA binding domain, excisionase family (TIGR01764; HMM-score: 19.8)
  and 2 more
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage-associated protein, BcepMu gp16 family (TIGR04111; HMM-score: 14)
  Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 12.9)
• TheSEED: see SAUSA300_pUSA030028
• PFAM:
  no clan defined Resolvase; Resolvase, N terminal domain (PF00239; HMM-score: 153.9)
  and 26 more
  HTH (CL0123) HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 33.5)
  HTH_11; HTH domain (PF08279; HMM-score: 26.5)
  HTH_23; Homeodomain-like domain (PF13384; HMM-score: 25.1)
  HTH_29; Winged helix-turn helix (PF13551; HMM-score: 23.1)
  HTH_32; Homeodomain-like domain (PF13565; HMM-score: 21.5)
  Mga; Mga helix-turn-helix domain (PF05043; HMM-score: 19.9)
  TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 19.5)
  HTH_22; HTH domain (PF13309; HMM-score: 19.3)
  HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 18.8)
  UPF0122; Putative helix-turn-helix protein, YlxM / p13 like (PF04297; HMM-score: 17)
  HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 17)
  Sigma70_r4; Sigma-70, region 4 (PF04545; HMM-score: 16.9)
  HTH_17; Helix-turn-helix domain (PF12728; HMM-score: 16.9)
  no clan defined DUF4277; Domain of unknown function (DUF4277) (PF14104; HMM-score: 16.5)
  HTH (CL0123) HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 15.8)
  Sigma70_r4_2; Sigma-70, region 4 (PF08281; HMM-score: 15.3)
  HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 14.9)
  HTH_10; HTH DNA binding domain (PF04967; HMM-score: 14.3)
  TnsD; Tn7-like transposition protein D (PF15978; HMM-score: 14.2)
  CENP-B_N; CENP-B N-terminal DNA-binding domain (PF04218; HMM-score: 13.9)
  CheY (CL0304) cREC_REC; Cyclic-phosphate processing Receiver domain (PF20274; HMM-score: 13.9)
  HTH (CL0123) HTH_Mga; M protein trans-acting positive regulator (MGA) HTH domain (PF08280; HMM-score: 13.5)
  DUF1804; Protein of unknown function (DUF1804) (PF08822; HMM-score: 13)
  LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 12.4)
  HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 12.4)
  HTH_IclR; IclR helix-turn-helix domain (PF09339; HMM-score: 11.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9921
  • Cytoplasmic Membrane Score: 0
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0077
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005178
  • TAT(Tat/SPI): 0.000495
  • LIPO(Sec/SPII): 0.00517
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447196603 NCBI
• RefSeq: WP_001273859 NCBI
• UniProt: see SAUSA300_pUSA030028

⊟Protein sequence

• MANIGYARVSTRDQSLDLQIDALSNFGCEKIFSEKVSGRKVNRTELDKCLDYLREGDTLVIYKLDRLGRTTKQLIDLAQWLENNNIELQIIDMNINTKDPMGKMFFTMMSAFAELEANLLSERTKKGLESARARGNMGGRKAMPQEKKEYIKHLYDTKEYTGQEIAKKTNVSRDTVYRILREFKK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications