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COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40
NCBI: 23-MAY-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_pUSA030028 [new locus tag: SAUSA300_RS14875 ]
- pan locus tag?:
- symbol: SAUSA300_pUSA030028
- pan gene symbol?: —
- synonym:
- product: resolvase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_pUSA030028 [new locus tag: SAUSA300_RS14875 ]
- symbol: SAUSA300_pUSA030028
- product: resolvase
- replicon: pUSA03
- strand: +
- coordinates: 26735..27367
- length: 633
- essential: unknown
⊟Accession numbers[edit | edit source]
- Gene ID: 3912728 NCBI
- RefSeq: YP_492714 NCBI
- BioCyc: GH3C-2662 BioCyc
- MicrobesOnline: 1291507 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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601TTGAGAATTAATTTTATAGATAACTATACATTAAAAACAATATACAACGCTATTTTAGAA
AGGATAATTACAATTATGGCCAATATTGGTTATGCTCGTGTATCTACCAGAGATCAAAGT
TTAGATTTACAAATAGACGCTTTGAGTAATTTTGGTTGTGAGAAAATATTTAGCGAAAAA
GTTAGTGGGCGTAAAGTAAATAGAACTGAACTAGATAAATGTTTAGATTATTTACGTGAA
GGCGACACTTTAGTTATTTACAAGCTAGATAGATTAGGCAGGACTACTAAGCAACTGATA
GATTTAGCCCAATGGTTAGAAAATAATAATATTGAATTACAAATTATTGATATGAATATC
AATACAAAAGACCCAATGGGAAAGATGTTTTTTACAATGATGAGTGCTTTTGCTGAATTA
GAGGCTAACTTATTAAGCGAAAGAACTAAAAAAGGTTTAGAATCAGCTAGAGCAAGAGGT
AACATGGGAGGCAGAAAAGCAATGCCACAAGAAAAAAAAGAATACATTAAACATTTATAC
GATACCAAAGAATATACAGGACAAGAAATTGCTAAAAAAACGAATGTAAGTAGAGATACT
GTATATCGCATTTTACGAGAGTTTAAAAAGTAA60
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633
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_pUSA030028 [new locus tag: SAUSA300_RS14875 ]
- symbol: SAUSA300_pUSA030028
- description: resolvase
- length: 210
- theoretical pI: 9.71682
- theoretical MW: 24442.1
- GRAVY: -0.534762
⊟Function[edit | edit source]
- TIGRFAM: RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 20.4)Unknown function General DNA binding domain, excisionase family (TIGR01764; HMM-score: 19.8)and 3 moreMobile and extrachromosomal element functions Prophage functions phage-associated protein, BcepMu gp16 family (TIGR04111; HMM-score: 13.9)RNA polymerase sigma-70 factor, Bacteroides expansion family 1 (TIGR02985; HMM-score: 12.9)transcriptional regulator BotR, P-21 (TIGR03209; HMM-score: 11.8)
- TheSEED :
- Resolvase/integrase
- PFAM: no clan defined Resolvase; Resolvase, N terminal domain (PF00239; HMM-score: 150.8)and 26 moreHTH (CL0123) HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 33)HTH_23; Homeodomain-like domain (PF13384; HMM-score: 26.9)HTH_11; HTH domain (PF08279; HMM-score: 25.7)HTH_29; Winged helix-turn helix (PF13551; HMM-score: 24.4)HTH_32; Homeodomain-like domain (PF13565; HMM-score: 23)TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 19.5)HTH_22; HTH domain (PF13309; HMM-score: 19.4)HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 19.3)Mga; Mga helix-turn-helix domain (PF05043; HMM-score: 18.6)HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 16.9)UPF0122; Putative helix-turn-helix protein, YlxM / p13 like (PF04297; HMM-score: 16.8)HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 16.6)Sigma70_r4; Sigma-70, region 4 (PF04545; HMM-score: 16.4)LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 16.1)no clan defined DUF4277; Domain of unknown function (DUF4277) (PF14104; HMM-score: 15.5)HTH (CL0123) HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 15)HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 14.4)CENP-B_N; CENP-B N-terminal DNA-binding domain (PF04218; HMM-score: 14.3)HTH_10; HTH DNA binding domain (PF04967; HMM-score: 14.1)Sigma70_r4_2; Sigma-70, region 4 (PF08281; HMM-score: 13.4)HTH_17; Helix-turn-helix domain (PF12728; HMM-score: 13)HTH_3; Helix-turn-helix (PF01381; HMM-score: 12.8)Rrf2; Transcriptional regulator (PF02082; HMM-score: 12.8)no clan defined TnsD; Tn7-like transposition protein D (PF15978; HMM-score: 12.7)HTH (CL0123) TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 12.4)HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 12)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.033789
- TAT(Tat/SPI): 0.001704
- LIPO(Sec/SPII): 0.002289
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MRINFIDNYTLKTIYNAILERIITIMANIGYARVSTRDQSLDLQIDALSNFGCEKIFSEKVSGRKVNRTELDKCLDYLREGDTLVIYKLDRLGRTTKQLIDLAQWLENNNIELQIIDMNINTKDPMGKMFFTMMSAFAELEANLLSERTKKGLESARARGNMGGRKAMPQEKKEYIKHLYDTKEYTGQEIAKKTNVSRDTVYRILREFKK
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: no data available
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]