From AureoWiki
Jump to navigation Jump to search
COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 23-MAY-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_pUSA030028 [new locus tag: SAUSA300_RS14875 ]
  • pan locus tag?:
  • symbol: SAUSA300_pUSA030028
  • pan gene symbol?: —
  • synonym:
  • product: resolvase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_pUSA030028 [new locus tag: SAUSA300_RS14875 ]
  • symbol: SAUSA300_pUSA030028
  • product: resolvase
  • replicon: pUSA03
  • strand: +
  • coordinates: 26735..27367
  • length: 633
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    TTGAGAATTAATTTTATAGATAACTATACATTAAAAACAATATACAACGCTATTTTAGAA
    AGGATAATTACAATTATGGCCAATATTGGTTATGCTCGTGTATCTACCAGAGATCAAAGT
    TTAGATTTACAAATAGACGCTTTGAGTAATTTTGGTTGTGAGAAAATATTTAGCGAAAAA
    GTTAGTGGGCGTAAAGTAAATAGAACTGAACTAGATAAATGTTTAGATTATTTACGTGAA
    GGCGACACTTTAGTTATTTACAAGCTAGATAGATTAGGCAGGACTACTAAGCAACTGATA
    GATTTAGCCCAATGGTTAGAAAATAATAATATTGAATTACAAATTATTGATATGAATATC
    AATACAAAAGACCCAATGGGAAAGATGTTTTTTACAATGATGAGTGCTTTTGCTGAATTA
    GAGGCTAACTTATTAAGCGAAAGAACTAAAAAAGGTTTAGAATCAGCTAGAGCAAGAGGT
    AACATGGGAGGCAGAAAAGCAATGCCACAAGAAAAAAAAGAATACATTAAACATTTATAC
    GATACCAAAGAATATACAGGACAAGAAATTGCTAAAAAAACGAATGTAAGTAGAGATACT
    GTATATCGCATTTTACGAGAGTTTAAAAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    633

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_pUSA030028 [new locus tag: SAUSA300_RS14875 ]
  • symbol: SAUSA300_pUSA030028
  • description: resolvase
  • length: 210
  • theoretical pI: 9.71682
  • theoretical MW: 24442.1
  • GRAVY: -0.534762

⊟Function[edit | edit source]

  • TIGRFAM:
    RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 20.4)
    Unknown function General DNA binding domain, excisionase family (TIGR01764; HMM-score: 19.8)
    and 3 more
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage-associated protein, BcepMu gp16 family (TIGR04111; HMM-score: 13.9)
    RNA polymerase sigma-70 factor, Bacteroides expansion family 1 (TIGR02985; HMM-score: 12.9)
    transcriptional regulator BotR, P-21 (TIGR03209; HMM-score: 11.8)
  • TheSEED  :
    • Resolvase/integrase
  • PFAM:
    no clan defined Resolvase; Resolvase, N terminal domain (PF00239; HMM-score: 153.4)
    and 26 more
    HTH (CL0123) HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 33.3)
    HTH_11; HTH domain (PF08279; HMM-score: 26.6)
    HTH_23; Homeodomain-like domain (PF13384; HMM-score: 24.9)
    HTH_29; Winged helix-turn helix (PF13551; HMM-score: 23.5)
    HTH_32; Homeodomain-like domain (PF13565; HMM-score: 21.3)
    Mga; Mga helix-turn-helix domain (PF05043; HMM-score: 19.7)
    TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 19.4)
    HTH_22; HTH domain (PF13309; HMM-score: 19.3)
    HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 18.6)
    Sigma70_r4; Sigma-70, region 4 (PF04545; HMM-score: 16.9)
    UPF0122; Putative helix-turn-helix protein, YlxM / p13 like (PF04297; HMM-score: 16.8)
    HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 16.7)
    LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 16.5)
    no clan defined DUF4277; Domain of unknown function (DUF4277) (PF14104; HMM-score: 16.2)
    HTH (CL0123) HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 16.1)
    HTH_17; Helix-turn-helix domain (PF12728; HMM-score: 16)
    Sigma70_r4_2; Sigma-70, region 4 (PF08281; HMM-score: 15)
    HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 14.9)
    HTH_10; HTH DNA binding domain (PF04967; HMM-score: 14.1)
    TnsD; Tn7-like transposition protein D (PF15978; HMM-score: 13.9)
    CENP-B_N; CENP-B N-terminal DNA-binding domain (PF04218; HMM-score: 13.7)
    CheY (CL0304) cREC_REC; Cyclic-phosphate processing Receiver domain (PF20274; HMM-score: 13.6)
    HTH (CL0123) HTH_Mga; M protein trans-acting positive regulator (MGA) HTH domain (PF08280; HMM-score: 13.3)
    DUF1804; Protein of unknown function (DUF1804) (PF08822; HMM-score: 12.9)
    TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 12.7)
    HTH_IclR; IclR helix-turn-helix domain (PF09339; HMM-score: 11.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9805
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0192
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.033789
    • TAT(Tat/SPI): 0.001704
    • LIPO(Sec/SPII): 0.002289
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRINFIDNYTLKTIYNAILERIITIMANIGYARVSTRDQSLDLQIDALSNFGCEKIFSEKVSGRKVNRTELDKCLDYLREGDTLVIYKLDRLGRTTKQLIDLAQWLENNNIELQIIDMNINTKDPMGKMFFTMMSAFAELEANLLSERTKKGLESARARGNMGGRKAMPQEKKEYIKHLYDTKEYTGQEIAKKTNVSRDTVYRILREFKK

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAUSA300_pUSA030028&oldid=97571"