COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 23-MAY-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_pUSA030006 [new locus tag: SAUSA300_RS14765 ]
• pan locus tag^?:
• symbol: SAUSA300_pUSA030006
• pan gene symbol^?: —
• synonym:
• product: replication and maintenance protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_pUSA030006 [new locus tag: SAUSA300_RS14765 ]
• symbol: SAUSA300_pUSA030006
• product: replication and maintenance protein
• replicon: pUSA03
• strand: +
• coordinates: 6681..7157
• length: 477
• essential: unknown

⊟Accession numbers

• Gene ID: 3912731 NCBI
• RefSeq: YP_492692 NCBI
• BioCyc: see SAUSA300_RS14765
• MicrobesOnline: 1291485 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAAGAAAGATATGGAACAGTCTATAAAGGCTCTCAGAGGCTCATAGACGAAGAAAGT
  GGAGAAGTCATAGAGGTAGACAAGTTATACCGTAAACAAACGTCTGGTAACTTCGTAAAG
  GCATATATAGTGCAATTAATAAGTATGTTAGATATGATTGGCGGAAAAAAACTTAAAATC
  GTTAACTATATCCTAGATAATGTCCACTTAAGTAACAATACAATGATAGCTACAACAAGA
  GAAATAGCAAAAGCTACAGGAACAAGTCTACAAACAGTAATAACAACACTTAAAATCTTA
  GAAGAAGGAAATATTATAAAAAGAAAAACTGGAGTATTAATGTTAAACCCTGAACTACTA
  ATGAGAGGCGACGACCAAAAACAAAAATACCTCTTACTCGAATTTGGGAACTTTGAGCAA
  GAGGCAAATGAAAAACAAGAAAATGCATTATCTGATTATTATTCTTTCAAGGACTAG
  60
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  477
  

⊟Protein

⊟General

• locus tag: SAUSA300_pUSA030006 [new locus tag: SAUSA300_RS14765 ]
• symbol: SAUSA300_pUSA030006
• description: replication and maintenance protein
• length: 158
• theoretical pI: 7.49276
• theoretical MW: 18078.7
• GRAVY: -0.411392

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 14.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other FeS assembly SUF system regulator (TIGR02944; HMM-score: 13.8)
  Signal transduction Regulatory functions DNA interactions FeS assembly SUF system regulator (TIGR02944; HMM-score: 13.8)
• TheSEED  :
  • Replication and maintenance protein
• PFAM:
  HTH (CL0123) RepL; Firmicute plasmid replication protein (RepL) (PF05732; HMM-score: 242)
  and 12 more
  HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 27.4)
  HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 24.1)
  FUR; Ferric uptake regulator family (PF01475; HMM-score: 16.7)
  MarR_2; MarR family (PF12802; HMM-score: 16.3)
  HTH_29; Winged helix-turn helix (PF13551; HMM-score: 15.6)
  HTH_11; HTH domain (PF08279; HMM-score: 15)
  MarR; MarR family (PF01047; HMM-score: 14.2)
  GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 13.4)
  HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 13)
  FaeA; FaeA-like protein (PF04703; HMM-score: 12.8)
  HTH_20; Helix-turn-helix domain (PF12840; HMM-score: 12.5)
  no clan defined PriA_3primeBD; 3'DNA-binding domain (3'BD) (PF17764; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9132
  • Cytoplasmic Membrane Score: 0.0219
  • Cell wall & surface Score: 0.0002
  • Extracellular Score: 0.0647
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002149
  • TAT(Tat/SPI): 0.000167
  • LIPO(Sec/SPII): 0.000509
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87159864 NCBI
• RefSeq: YP_492692 NCBI
• UniProt: A0A0H2XKQ1 UniProt

⊟Protein sequence

• MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQLISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGNIIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications