COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS00035 [old locus tag: SAP009 ]
• pan locus tag^?:
• symbol: SA_RS00035
• pan gene symbol^?: —
• synonym:
• product: MarR family transcriptional regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS00035 [old locus tag: SAP009 ]
• symbol: SA_RS00035
• product: MarR family transcriptional regulator
• replicon: pN315
• strand: +
• coordinates: 7000..7419
• length: 420
• essential: unknown

⊟Accession numbers

• Location: NC_003140 (7000..7419) NCBI
• BioCyc: SA_RS00035 BioCyc
• MicrobesOnline: see SAP009

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  GTGGAATTAGATAATTGTATAAATTATTTGTTGAGTACTTCTCAGAATAGAGTTTTTAAA
  CATTTTAAAAGTTTATTAAGTCCCTATAATATTACTCCAGCAGAATATGGTGTTTTGAAT
  TGCTTGATTAATAGTAATTTGAATACACCTAAAGCGATTGGTAATACTTTAAATTTAGAA
  CCTTCAACAATATCTGGAATATTAGATAAGATGTCCAAAAAGGATCTAATAACACGTCAA
  ACTGATAATGAAAATAGAAGAACTGTGCTAATAAACAGTACAAATAAAGCAAAAGACTTA
  TGGCCAACATTAGAAAAGAAAGCCTATCAACTTAATAATCATTACTTCGCTAATTTAACT
  AATGAAGAAACTGTAGTGTTTAAAAAGATATTACTAAAGATAAATAAAACTACGTTTTAA
  60
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  420
  

⊟Protein

⊟General

• locus tag: SA_RS00035 [old locus tag: SAP009 ]
• symbol: SA_RS00035
• description: MarR family transcriptional regulator
• length: 139
• theoretical pI: 9.83609
• theoretical MW: 15938.2
• GRAVY: -0.443165

⊟Function

• TIGRFAM:
  mobile rSAM pair MarR family regulator (TIGR04472; HMM-score: 35.8)
  homoprotocatechuate degradation operon regulator, HpaR (TIGR02337; HMM-score: 35.4)
  and 1 more
  Signal transduction Regulatory functions DNA interactions staphylococcal accessory regulator family (TIGR01889; HMM-score: 24.6)
• TheSEED: see SAP009
• PFAM:
  HTH (CL0123) MarR; MarR family (PF01047; HMM-score: 43.6)
  MarR_2; MarR family (PF12802; HMM-score: 38.1)
  and 7 more
  HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 30.2)
  HTH_micro; HTH-like (PF17007; HMM-score: 23.4)
  TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 21.9)
  HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 15)
  HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 13.7)
  Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 13.1)
  no clan defined DUF371; Domain of unknown function (DUF371) (PF04027; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.011275
  • TAT(Tat/SPI): 0.000275
  • LIPO(Sec/SPII): 0.000726
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446340180 NCBI
• RefSeq: WP_000418035 NCBI
• UniProt: see SAP009

⊟Protein sequence

• MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINSNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTLEKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications