COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS00090 [old locus tag: SAP020 ]
• pan locus tag^?:
• symbol: SA_RS00090
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS00090 [old locus tag: SAP020 ]
• symbol: SA_RS00090
• product: hypothetical protein
• replicon: pN315
• strand: +
• coordinates: 16871..18805
• length: 1935
• essential: unknown

⊟Accession numbers

• Location: NC_003140 (16871..18805) NCBI
• BioCyc: SA_RS00090 BioCyc
• MicrobesOnline: see SAP020

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  1741
  1801
  1861
  1921
  
  ATGAAAAAATTCAAATTATTTTTAGATTTTAGTACTTTATTATTAATTTCAGGGCTATTA
  TTTTTATTTTTCTTCAAAGAAAATGAAGAAATTATACCTGAAAGTTCCAATATTTTAACA
  ATCTCTAATTGGGATAAATCTAACAGTAAATCAAAAGTGTTAGATGTCATTGAATCAGGC
  GCCAAAAATCAAAATATACAAATAATTAAATCCGTTAAAGATTTTGATAATAAAAAAGAA
  TTTTTTGTTTTCAACTCAAAACGAAATAATTCTGACTTTATACGTAATAAAACATCACTT
  TTAACTCCATCAGATTTATTAAACAGAGAGATAAAAGGAAAGTATTATATTATTGGCGAA
  CATTTCAATGTAGAAGAGCTTACGAGTGAATTAAAAAAAGCTGGTTTAACATCTGAAGTT
  GAATACATAAATAGAAACATATTATTTTTTGAATTAGTCATAGATAATGATTTATTAGTG
  CCTTTCATATTCTTAGGCGTATTATATCTTTTGTACTTCTTATATGACAGAGGATATAAT
  TTGAAATTTTATGCTATTCAAAAGCTACATGGCTTTTCAACTACACGCATTATTTTTCAT
  AATATTCATATAAAAATCATTTATTGGCTAGTACTTACCACTATTTTCTTTATTGCTAAT
  ATATTAATCATTCATATAGCCAATTTAGAATTGGATTTCTTAATGTTTATTCGTAGATTT
  ATCGTGTTGCTTTTTGTTTTTGCTTGTATTACTACATTGTTGTGGCTGATTTCGTATAGT
  CTTCTTTTGAAGTTAAGTATTCCATTAACCTTAAAAGGGAAAAAAGGATATAAGTTTTCA
  ATATTTATGGGTACCTTCACCAAATGTATCATGGTGCTAATACTATCTTTTTTACTGGCA
  CAAAATTTAAATGCTTATACGAAGTTAAAGAATATATATGACTCTGAAAAAATCTGGCAA
  AAGTTAGATAATTACTATACATTAGAGATTTCTCCTAATATTAGAAATTCAGAAGAAAAA
  CAAATTTTAGAAACTAATATATACAATTTAATTAAGAAAAGCGAACAACTTGGTGGATTG
  CTTTTAAAGAATAATAATATAGCCAATCCCGATAAAAACAATTACATTCCTGAAAATGGC
  AATGTCTTTTTTATCAATAACAATTTTCTGAATTTCTATAAAAATATAAATCATGATTTT
  AAAATGATTGACAATCATTCGGATAAAATAAACGTGTTTATTCCTCCAAGGTTACAATAT
  CAAAAATCTGAAATTCAAAAAAATCACCAAGGATGGATTGACTTTCAAAAGCAACAAAAC
  AAAAAAGTAGATGTGCAAGTACTGTCAAAAAACGTATCTGTTTTTTCTTTTGATCGCACA
  ACTAATTTGAAATTTGGATATCTAGATTCTCCAATAGTAATGTTGTTGACACCTGACGAT
  TTAACAGGAGATTTTTATTTAGCTGCTGTTTCTCAAGGTGGATATCTATTTAAAGATTTA
  GATACACTTAAACAGTTGCTTAAAGATAATCACCTAGAAGAAGAAATTAGCGGTATAACT
  AATTACAAGGATTCTGTGTTAAATGAATTAAATGAAACGAAAACAAAAATGATAATAACA
  ATAGTTACTATTATTATTAACGTTTTCATTCTTTTGATAGCTACTGTATTTGAAACAATT
  CAATATTTTAGCTGGAATAAAAAGCAACTATTATTAAGAAAAATTCATGGTTATTCATTA
  TTCAGCAGTAATGTACGTTATTTAACAATTTCTATTTTACTTTCTATTATGTTAGCTTAC
  ACTACGCATATTTTATTTGGTTCAAAGATATTACTATTCATAATAATGTCTATTGCTATT
  ATTCAACATTTACTTCAAATTGCTTATATTAAATATCTTGAAAAATACTTTAAAGATTTA
  ATGAGGGAGATTTAA
  60
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  240
  300
  360
  420
  480
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  840
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  1140
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  1320
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  1560
  1620
  1680
  1740
  1800
  1860
  1920
  1935
  

⊟Protein

⊟General

• locus tag: SA_RS00090 [old locus tag: SAP020 ]
• symbol: SA_RS00090
• description: hypothetical protein
• length: 644
• theoretical pI: 9.57653
• theoretical MW: 75479
• GRAVY: 0.131677

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Toxin production and resistance bacteriocin-associated integral membrane protein (TIGR01654; HMM-score: 238)
• TheSEED: see SAP020
• PFAM:
  BPD_transp_1 (CL0404) DUF1430; Protein of unknown function (DUF1430) (PF07242; HMM-score: 69.2)
  and 3 more
  no clan defined CD34_antigen; CD34/Podocalyxin family (PF06365; HMM-score: 22.7)
  Eaf7; Chromatin modification-related protein EAF7 (PF07904; HMM-score: 15)
  Cache (CL0165) DUF2222; Uncharacterised signal transduction histidine kinase domain (DUF2222) (PF09984; HMM-score: 14.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 7
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.171789
  • TAT(Tat/SPI): 0.000753
  • LIPO(Sec/SPII): 0.03148
• predicted transmembrane helices (TMHMM): 8

⊟Accession numbers

• GI: 446636385 NCBI
• RefSeq: WP_000713731 NCBI
• UniProt: see SAP020

⊟Protein sequence

• MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNWDKSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLTPSDLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLVPFIFLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTTIFFIANILIIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLTLKGKKGYKFSIFMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNIRNSEEKQILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNFYKNINHDFKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSKNVSVFSFDRTTNLKFGYLDSPIVMLLTPDDLTGDFYLAAVSQGGYLFKDLDTLKQLLKDNHLEEEISGITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWNKKQLLLRKIHGYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQHLLQIAYIKYLEKYFKDLMREI

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications