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COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 27-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SAP020 [new locus tag: SA_RS00090 ]
  • pan locus tag?:
  • symbol: SAP020
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAP020 [new locus tag: SA_RS00090 ]
  • symbol: SAP020
  • product: hypothetical protein
  • replicon: pN315
  • strand: +
  • coordinates: 16871..18805
  • length: 1935
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAAAAAATTCAAATTATTTTTAGATTTTAGTACTTTATTATTAATTTCAGGGCTATTA
    TTTTTATTTTTCTTCAAAGAAAATGAAGAAATTATACCTGAAAGTTCCAATATTTTAACA
    ATCTCTAATTGGGATAAATCTAACAGTAAATCAAAAGTGTTAGATGTCATTGAATCAGGC
    GCCAAAAATCAAAATATACAAATAATTAAATCCGTTAAAGATTTTGATAATAAAAAAGAA
    TTTTTTGTTTTCAACTCAAAACGAAATAATTCTGACTTTATACGTAATAAAACATCACTT
    TTAACTCCATCAGATTTATTAAACAGAGAGATAAAAGGAAAGTATTATATTATTGGCGAA
    CATTTCAATGTAGAAGAGCTTACGAGTGAATTAAAAAAAGCTGGTTTAACATCTGAAGTT
    GAATACATAAATAGAAACATATTATTTTTTGAATTAGTCATAGATAATGATTTATTAGTG
    CCTTTCATATTCTTAGGCGTATTATATCTTTTGTACTTCTTATATGACAGAGGATATAAT
    TTGAAATTTTATGCTATTCAAAAGCTACATGGCTTTTCAACTACACGCATTATTTTTCAT
    AATATTCATATAAAAATCATTTATTGGCTAGTACTTACCACTATTTTCTTTATTGCTAAT
    ATATTAATCATTCATATAGCCAATTTAGAATTGGATTTCTTAATGTTTATTCGTAGATTT
    ATCGTGTTGCTTTTTGTTTTTGCTTGTATTACTACATTGTTGTGGCTGATTTCGTATAGT
    CTTCTTTTGAAGTTAAGTATTCCATTAACCTTAAAAGGGAAAAAAGGATATAAGTTTTCA
    ATATTTATGGGTACCTTCACCAAATGTATCATGGTGCTAATACTATCTTTTTTACTGGCA
    CAAAATTTAAATGCTTATACGAAGTTAAAGAATATATATGACTCTGAAAAAATCTGGCAA
    AAGTTAGATAATTACTATACATTAGAGATTTCTCCTAATATTAGAAATTCAGAAGAAAAA
    CAAATTTTAGAAACTAATATATACAATTTAATTAAGAAAAGCGAACAACTTGGTGGATTG
    CTTTTAAAGAATAATAATATAGCCAATCCCGATAAAAACAATTACATTCCTGAAAATGGC
    AATGTCTTTTTTATCAATAACAATTTTCTGAATTTCTATAAAAATATAAATCATGATTTT
    AAAATGATTGACAATCATTCGGATAAAATAAACGTGTTTATTCCTCCAAGGTTACAATAT
    CAAAAATCTGAAATTCAAAAAAATCACCAAGGATGGATTGACTTTCAAAAGCAACAAAAC
    AAAAAAGTAGATGTGCAAGTACTGTCAAAAAACGTATCTGTTTTTTCTTTTGATCGCACA
    ACTAATTTGAAATTTGGATATCTAGATTCTCCAATAGTAATGTTGTTGACACCTGACGAT
    TTAACAGGAGATTTTTATTTAGCTGCTGTTTCTCAAGGTGGATATCTATTTAAAGATTTA
    GATACACTTAAACAGTTGCTTAAAGATAATCACCTAGAAGAAGAAATTAGCGGTATAACT
    AATTACAAGGATTCTGTGTTAAATGAATTAAATGAAACGAAAACAAAAATGATAATAACA
    ATAGTTACTATTATTATTAACGTTTTCATTCTTTTGATAGCTACTGTATTTGAAACAATT
    CAATATTTTAGCTGGAATAAAAAGCAACTATTATTAAGAAAAATTCATGGTTATTCATTA
    TTCAGCAGTAATGTACGTTATTTAACAATTTCTATTTTACTTTCTATTATGTTAGCTTAC
    ACTACGCATATTTTATTTGGTTCAAAGATATTACTATTCATAATAATGTCTATTGCTATT
    ATTCAACATTTACTTCAAATTGCTTATATTAAATATCTTGAAAAATACTTTAAAGATTTA
    ATGAGGGAGATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1935

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAP020 [new locus tag: SA_RS00090 ]
  • symbol: SAP020
  • description: hypothetical protein
  • length: 644
  • theoretical pI: 9.57653
  • theoretical MW: 75479
  • GRAVY: 0.131677

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Toxin production and resistance bacteriocin-associated integral membrane protein (TIGR01654; HMM-score: 238)
  • TheSEED  :
    • immunity protein, probable
  • PFAM:
    BPD_transp_1 (CL0404) DUF1430; Protein of unknown function (DUF1430) (PF07242; HMM-score: 69.2)
    and 3 more
    no clan defined CD34_antigen; CD34/Podocalyxin family (PF06365; HMM-score: 22.7)
    Eaf7; Chromatin modification-related protein EAF7 (PF07904; HMM-score: 15)
    Cache (CL0165) DUF2222; Uncharacterised signal transduction histidine kinase domain (DUF2222) (PF09984; HMM-score: 14.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 7
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.171789
    • TAT(Tat/SPI): 0.000753
    • LIPO(Sec/SPII): 0.03148
  • predicted transmembrane helices (TMHMM): 8

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNWDKSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLTPSDLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLVPFIFLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTTIFFIANILIIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLTLKGKKGYKFSIFMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNIRNSEEKQILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNFYKNINHDFKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSKNVSVFSFDRTTNLKFGYLDSPIVMLLTPDDLTGDFYLAAVSQGGYLFKDLDTLKQLLKDNHLEEEISGITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWNKKQLLLRKIHGYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQHLLQIAYIKYLEKYFKDLMREI

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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