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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS00475 [old locus tag: SA0066 ]
  • pan locus tag?: SAUPAN000454000
  • symbol: SA_RS00475
  • pan gene symbol?: kdpE
  • synonym:
  • product: DNA-binding response regulator

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS00475 [old locus tag: SA0066 ]
  • symbol: SA_RS00475
  • product: DNA-binding response regulator
  • replicon: chromosome
  • strand: -
  • coordinates: 73509..74204
  • length: 696
  • essential: no DEG

Accession numbers[edit | edit source]

  • Location: NC_002745 (73509..74204) NCBI
  • BioCyc: SA_RS00475 BioCyc
  • MicrobesOnline: see SA0066

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAAACAACATTATTGGTCGTTGAAGATGATGAAGCGATTTTACATTTAATCGATGTT
    GCGCTAACTATGAATTATTATAAAGTGGTTACGGCGAAGACGGGGAAAGAAGCGGACTTT
    CGCCTAAGAACAGAACAACCAGATATTATCTTATTAGATTTAGGCTTACCAGATATTGAT
    GGTTTATCTCTCATTCAGCAATTCAGAGACTTTGTGGACACACCTATTATTGTGATTAGT
    GCACGAACAGAGGAACAAACCATTGTCGAAGTATTAGATCGCGGTGCCAATGATTATATG
    ACTAAGCCTTTCAATATCGATGAGTTAAGAGCACGTATCAGAGTAGCATTACGTATGTCA
    CGTTCTACTGAGACAATTCAAAGTCGTTTCACAAATGGTCCATTAACCATTGATTTCGAT
    GCGAAATCAGTCCTTGTCAATGAACAGTCCGTACATCTAACACCGAATGAATTCCAACTC
    CTAACGATATTATGCCATCATGTCGGCAAAGTATTAACCTATCAAGTGTTATTAAAATCA
    CTCTATGGCTATGTCAATCATACAGAAATGGCTGCCTTACGTGTACATATGGCCTCTTTA
    CGTAAAAAGTTGAATTATACGCAAGACCCAAACACTGAAAAGAAATTAATCATCACACAT
    CCTAGAATCGGCTACCAAATGCAACACTTAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    696

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS00475 [old locus tag: SA0066 ]
  • symbol: SA_RS00475
  • description: DNA-binding response regulator
  • length: 231
  • theoretical pI: 6.51567
  • theoretical MW: 26491.5
  • GRAVY: -0.134632

Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 161.9)
    Signal transduction Signal transduction Two-component systems phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 161.9)
    and 8 more
    Signal transduction Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 121.3)
    proteobacterial dedicated sortase system response regulator (TIGR03787; HMM-score: 90.9)
    Metabolism Central intermediary metabolism Nitrogen metabolism nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 71.7)
    Signal transduction Regulatory functions DNA interactions nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 71.7)
    Signal transduction Signal transduction Two-component systems nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 71.7)
    Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 59.7)
    Cellular processes Cellular processes Sporulation and germination sporulation transcription factor Spo0A (TIGR02875; HMM-score: 52.1)
    Signal transduction Regulatory functions DNA interactions PEP-CTERM-box response regulator transcription factor (TIGR02915; HMM-score: 49.8)
  • TheSEED: see SA0066
  • PFAM:
    CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 92.7)
    and 1 more
    HTH (CL0123) Trans_reg_C; Transcriptional regulatory protein, C terminal (PF00486; HMM-score: 71.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002415
    • TAT(Tat/SPI): 0.000103
    • LIPO(Sec/SPII): 0.000275
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKTTLLVVEDDEAILHLIDVALTMNYYKVVTAKTGKEADFRLRTEQPDIILLDLGLPDIDGLSLIQQFRDFVDTPIIVISARTEEQTIVEVLDRGANDYMTKPFNIDELRARIRVALRMSRSTETIQSRFTNGPLTIDFDAKSVLVNEQSVHLTPNEFQLLTILCHHVGKVLTYQVLLKSLYGYVNHTEMAALRVHMASLRKKLNYTQDPNTEKKLIITHPRIGYQMQHLE

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]