PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS00490 [old locus tag: SA0068 ]
• pan locus tag^?: SAUPAN000456000
• symbol: SA_RS00490
• pan gene symbol^?: kdpA
• synonym:
• product: potassium-transporting ATPase subunit A

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS00490 [old locus tag: SA0068 ]
• symbol: SA_RS00490
• product: potassium-transporting ATPase subunit A
• replicon: chromosome
• strand: +
• coordinates: 77116..78792
• length: 1677
• essential: no DEG

⊟Accession numbers

• Location: NC_002745 (77116..78792) NCBI
• BioCyc: SA_RS00490 BioCyc
• MicrobesOnline: see SA0068

⊟Phenotype

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⊟DNA sequence

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  ATGAGTATTGTGTTGTTTTTGATTGTATTTATCTTGCTCTCACTCATTGTGAGCCGATAT
  TTATATTCAGTTGCTTTAAATGTGCCATCTAAAATAGATGTTGTTTTTAATCCGATTGAG
  AAATTGATTTATCAACTGATTGGCACGAAATTAGAACACATGTCTGGGAAGACGTATATC
  AAACATTTTTTGTTGTTTAACGGATTGATGGGCGGATTGTCCTTTGTATTATTGCTTATT
  CAACAATGGCTGTTTTTGAATCCTAACCATAATTTAAATCAATCTGTATCGTTAGCCTTT
  AATACTATGGCATCTTTTTTGACCAATACTAACTTACAGCATTATGCAGGTGAAACAGAT
  TTAAGTTATTTAACACAAATGTGTGTCATCACTTTCTTAATGTTCACGTCAGCAGCGTCA
  GGTTACGCCGTATGTATTGCGATGTTAAGACGTTTGACTGGAATGACAGATGTGATTGGT
  AATTTCTATCAAGATATTACGCGTTTTATTGTACGGGTGCTCATACCTTTCGCATTGATC
  ATCAGTTTGTTTTTAATCAGTCAGGGCACACCGCAAACGCTTAAAGGTAATTTGGTGATT
  GAGACATTATCAGGTGTGAAACAAACGATTGCATATGGACCGATGGCGTCTTTAGAATCT
  ATTAAACATTTAGGGACAAATGGTGGTGGTTTCTTAGGTGCGAACTCTTCTACACCTTTT
  GAAAATCCGACATACTGGTCTAATTACGCTGAAGCTTTAAGTATGATGTTGATTCCAGGT
  TCATTAGTCTTTCTATTCGGTAGAATGTTGAAAACTAAACTACAGATTCATCCGCATGCG
  ATTATGATTTTCGTTGCGATGTTTGTAATGTTCATCGGCTTTTTAGTGACATGTCTCTAT
  TTTGAATTTGCGGGGAATCCAGTGTTGCATCACTTAGGTATTGCCGGTGGCAATATGGAA
  GGCAAAGAAACACGTTTCGGTATTGGCTTATCCGCTTTATTTACAACCATTACGACCGCT
  TTTACTACAGGAACAGTTAACAATATGCACGATAGTCTTACACCGCTAGGCGGCATGGTT
  CCAATGGTATTAATGATGTTGAATGCAGTTTTTGGCGGTGAAGGTGTTGGGCTGATGAAC
  ATGTTGATTTATGTCATGTTAACGGTCTTTATCTGTAGTTTGATGATTGGGAAAACACCA
  AGTTATTTAGGAATGAAGATTGAAGGTAAAGAGATGAAACTCATTGCGCTTTCTTTCTTA
  GTACATCCTTTACTTATTTTGGTTTTTTCAGCACTAGCTTTTATTGTGCCAGGGGCATCA
  GATGCGTTAACTAATCCGCAATTCCACGGTGTATCACAAGTGTTGTATGAGTTTACATCA
  TCTTCAGCGAATAATGGCTCTGGTTTTGAAGGATTAGGAGACAATACGGTATTTTGGAAC
  ATTTCAACAGGCATTGTGATGTTGCTTGCACGATATATTCCAATCGTTTTACAAATTTTG
  ATTGTATCTAGTTTGGTAAATAAAAAGACCTATCAGCAACATACTCAAGATGTACCGATT
  AATAATTTATTTTTCAGCAGTGTATTGATTATCTTTATTATTTTGTTGAGCGGCTTAACG
  TTCTTACCTGACTTAATGCTTGGACCAATAGGCGAACAGCTTTTGCTGCACGCATAG
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⊟Protein

⊟General

• locus tag: SA_RS00490 [old locus tag: SA0068 ]
• symbol: SA_RS00490
• description: potassium-transporting ATPase subunit A
• length: 558
• theoretical pI: 7.72916
• theoretical MW: 61262.4
• GRAVY: 0.72276

⊟Function

• reaction:
  EC 3.6.3.12?  ExPASy
  Potassium-transporting ATPase ATP + H₂O + K⁺(Out) = ADP + phosphate + K⁺(In)
• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds K+-transporting ATPase, A subunit (TIGR00680; EC 3.6.3.12; HMM-score: 588.1)
• TheSEED: see SA0068
• PFAM:
  Ion_channel (CL0030) KdpA; Potassium-transporting ATPase A subunit (PF03814; HMM-score: 716.7)
  and 1 more
  CopD_like (CL0430) DUF4149; Domain of unknown function (DUF4149) (PF13664; HMM-score: 14.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.256215
  • TAT(Tat/SPI): 0.002539
  • LIPO(Sec/SPII): 0.025806
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 445951575 NCBI
• RefSeq: WP_000029430 NCBI
• UniProt: see SA0068

⊟Protein sequence

• MSIVLFLIVFILLSLIVSRYLYSVALNVPSKIDVVFNPIEKLIYQLIGTKLEHMSGKTYIKHFLLFNGLMGGLSFVLLLIQQWLFLNPNHNLNQSVSLAFNTMASFLTNTNLQHYAGETDLSYLTQMCVITFLMFTSAASGYAVCIAMLRRLTGMTDVIGNFYQDITRFIVRVLIPFALIISLFLISQGTPQTLKGNLVIETLSGVKQTIAYGPMASLESIKHLGTNGGGFLGANSSTPFENPTYWSNYAEALSMMLIPGSLVFLFGRMLKTKLQIHPHAIMIFVAMFVMFIGFLVTCLYFEFAGNPVLHHLGIAGGNMEGKETRFGIGLSALFTTITTAFTTGTVNNMHDSLTPLGGMVPMVLMMLNAVFGGEGVGLMNMLIYVMLTVFICSLMIGKTPSYLGMKIEGKEMKLIALSFLVHPLLILVFSALAFIVPGASDALTNPQFHGVSQVLYEFTSSSANNGSGFEGLGDNTVFWNISTGIVMLLARYIPIVLQILIVSSLVNKKTYQQHTQDVPINNLFFSSVLIIFIILLSGLTFLPDLMLGPIGEQLLLHA

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications