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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0068 [new locus tag: SA_RS00490 ]
  • pan locus tag?: SAUPAN000456000
  • symbol: kdpA
  • pan gene symbol?: kdpA
  • synonym:
  • product: potassium-transporting ATPase subunit A

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0068 [new locus tag: SA_RS00490 ]
  • symbol: kdpA
  • product: potassium-transporting ATPase subunit A
  • replicon: chromosome
  • strand: +
  • coordinates: 77116..78792
  • length: 1677
  • essential: no DEG

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAGTATTGTGTTGTTTTTGATTGTATTTATCTTGCTCTCACTCATTGTGAGCCGATAT
    TTATATTCAGTTGCTTTAAATGTGCCATCTAAAATAGATGTTGTTTTTAATCCGATTGAG
    AAATTGATTTATCAACTGATTGGCACGAAATTAGAACACATGTCTGGGAAGACGTATATC
    AAACATTTTTTGTTGTTTAACGGATTGATGGGCGGATTGTCCTTTGTATTATTGCTTATT
    CAACAATGGCTGTTTTTGAATCCTAACCATAATTTAAATCAATCTGTATCGTTAGCCTTT
    AATACTATGGCATCTTTTTTGACCAATACTAACTTACAGCATTATGCAGGTGAAACAGAT
    TTAAGTTATTTAACACAAATGTGTGTCATCACTTTCTTAATGTTCACGTCAGCAGCGTCA
    GGTTACGCCGTATGTATTGCGATGTTAAGACGTTTGACTGGAATGACAGATGTGATTGGT
    AATTTCTATCAAGATATTACGCGTTTTATTGTACGGGTGCTCATACCTTTCGCATTGATC
    ATCAGTTTGTTTTTAATCAGTCAGGGCACACCGCAAACGCTTAAAGGTAATTTGGTGATT
    GAGACATTATCAGGTGTGAAACAAACGATTGCATATGGACCGATGGCGTCTTTAGAATCT
    ATTAAACATTTAGGGACAAATGGTGGTGGTTTCTTAGGTGCGAACTCTTCTACACCTTTT
    GAAAATCCGACATACTGGTCTAATTACGCTGAAGCTTTAAGTATGATGTTGATTCCAGGT
    TCATTAGTCTTTCTATTCGGTAGAATGTTGAAAACTAAACTACAGATTCATCCGCATGCG
    ATTATGATTTTCGTTGCGATGTTTGTAATGTTCATCGGCTTTTTAGTGACATGTCTCTAT
    TTTGAATTTGCGGGGAATCCAGTGTTGCATCACTTAGGTATTGCCGGTGGCAATATGGAA
    GGCAAAGAAACACGTTTCGGTATTGGCTTATCCGCTTTATTTACAACCATTACGACCGCT
    TTTACTACAGGAACAGTTAACAATATGCACGATAGTCTTACACCGCTAGGCGGCATGGTT
    CCAATGGTATTAATGATGTTGAATGCAGTTTTTGGCGGTGAAGGTGTTGGGCTGATGAAC
    ATGTTGATTTATGTCATGTTAACGGTCTTTATCTGTAGTTTGATGATTGGGAAAACACCA
    AGTTATTTAGGAATGAAGATTGAAGGTAAAGAGATGAAACTCATTGCGCTTTCTTTCTTA
    GTACATCCTTTACTTATTTTGGTTTTTTCAGCACTAGCTTTTATTGTGCCAGGGGCATCA
    GATGCGTTAACTAATCCGCAATTCCACGGTGTATCACAAGTGTTGTATGAGTTTACATCA
    TCTTCAGCGAATAATGGCTCTGGTTTTGAAGGATTAGGAGACAATACGGTATTTTGGAAC
    ATTTCAACAGGCATTGTGATGTTGCTTGCACGATATATTCCAATCGTTTTACAAATTTTG
    ATTGTATCTAGTTTGGTAAATAAAAAGACCTATCAGCAACATACTCAAGATGTACCGATT
    AATAATTTATTTTTCAGCAGTGTATTGATTATCTTTATTATTTTGTTGAGCGGCTTAACG
    TTCTTACCTGACTTAATGCTTGGACCAATAGGCGAACAGCTTTTGCTGCACGCATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1677

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0068 [new locus tag: SA_RS00490 ]
  • symbol: KdpA
  • description: potassium-transporting ATPase subunit A
  • length: 558
  • theoretical pI: 7.72916
  • theoretical MW: 61262.4
  • GRAVY: 0.72276

⊟Function[edit | edit source]

  • reaction:
    EC 3.6.3.12?  ExPASy
    Potassium-transporting ATPase ATP + H2O + K+(Out) = ADP + phosphate + K+(In)
  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds K+-transporting ATPase, A subunit (TIGR00680; EC 3.6.3.12; HMM-score: 588.1)
  • TheSEED  :
    • K+-transporting ATPase chain A (EC 7.2.2.6)
    Potassium metabolism Potassium metabolism - no subcategory Potassium homeostasis  Potassium-transporting ATPase A chain (EC 3.6.3.12) (TC 3.A.3.7.1)
  • PFAM:
    Ion_channel (CL0030) KdpA; Potassium-transporting ATPase A subunit (PF03814; HMM-score: 716.7)
    and 1 more
    CopD_like (CL0430) DUF4149; Domain of unknown function (DUF4149) (PF13664; HMM-score: 14.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.256215
    • TAT(Tat/SPI): 0.002539
    • LIPO(Sec/SPII): 0.025806
  • predicted transmembrane helices (TMHMM): 12

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSIVLFLIVFILLSLIVSRYLYSVALNVPSKIDVVFNPIEKLIYQLIGTKLEHMSGKTYIKHFLLFNGLMGGLSFVLLLIQQWLFLNPNHNLNQSVSLAFNTMASFLTNTNLQHYAGETDLSYLTQMCVITFLMFTSAASGYAVCIAMLRRLTGMTDVIGNFYQDITRFIVRVLIPFALIISLFLISQGTPQTLKGNLVIETLSGVKQTIAYGPMASLESIKHLGTNGGGFLGANSSTPFENPTYWSNYAEALSMMLIPGSLVFLFGRMLKTKLQIHPHAIMIFVAMFVMFIGFLVTCLYFEFAGNPVLHHLGIAGGNMEGKETRFGIGLSALFTTITTAFTTGTVNNMHDSLTPLGGMVPMVLMMLNAVFGGEGVGLMNMLIYVMLTVFICSLMIGKTPSYLGMKIEGKEMKLIALSFLVHPLLILVFSALAFIVPGASDALTNPQFHGVSQVLYEFTSSSANNGSGFEGLGDNTVFWNISTGIVMLLARYIPIVLQILIVSSLVNKKTYQQHTQDVPINNLFFSSVLIIFIILLSGLTFLPDLMLGPIGEQLLLHA

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

T Ito, Y Katayama, K Hiramatsu
Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315.
Antimicrob Agents Chemother: 1999, 43(6);1449-58
[PubMed:10348769] [WorldCat.org] [DOI] (P p)

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