From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS01250 [old locus tag: SA0213 ]
  • pan locus tag?: SAUPAN001073000
  • symbol: SA_RS01250
  • pan gene symbol?:
  • synonym:
  • product: membrane protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS01250 [old locus tag: SA0213 ]
  • symbol: SA_RS01250
  • product: membrane protein
  • replicon: chromosome
  • strand: -
  • coordinates: 252541..253113
  • length: 573
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (252541..253113) NCBI
  • BioCyc: G1G21-227 BioCyc
  • MicrobesOnline: see SA0213

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    TTGACAAGTTTTATCTATAAAATACTTTATGTTGTCAAAATCAACGCTTATACATATGAC
    ATAATGACGGAGGACATCATGATTCTATCTATATTACTTATCTTTTTCTGTATCAGACTT
    GTCAGCTTAAAGATATCTATTAATCACTCAAAACAATTAAAAGCAGACGGTGCAGTTGAA
    TATGGCGTTAAAAATTCGAAGTTTTTAGCAATAACACACGTTTTAATTTATGTATTGGCT
    GGTGTAGAGGCATTTATTAATAAAGATACATTTAGCTTTGCAAATGGTATTGGCTTAGTT
    ATATTAATCTTTGCTTATATCATGTTATTTATGGTTATTAAAACTTTAGGTGGTATTTGG
    ACATTAAAATTATTCATTTTACCTAACCATCCTATTATTAAATCTGGATTATATAAAATT
    ACAAAACACCCAAATTACTTCTTAAACATCATTCCTGAATTAATCGGTGTATTATTATTA
    ACACACGCAACATATACAACTATTTTATTAGTACCGTATGCGTATTTCTTATATGTACGT
    ATTAAACAAGAAGAGAAATTAATGAATATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    573

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS01250 [old locus tag: SA0213 ]
  • symbol: SA_RS01250
  • description: membrane protein
  • length: 190
  • theoretical pI: 9.73661
  • theoretical MW: 21779.2
  • GRAVY: 0.823684

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: see SA0213
  • PFAM:
    Steroid_dh (CL0115) ICMT; Isoprenylcysteine carboxyl methyltransferase (ICMT) family (PF04140; HMM-score: 105.7)
    and 3 more
    PEMT; Phospholipid methyltransferase (PF04191; HMM-score: 25.6)
    DUF1295; Protein of unknown function (DUF1295) (PF06966; HMM-score: 17.9)
    Transthyretin (CL0287) DUF2606; Protein of unknown function (DUF2606) (PF10794; HMM-score: 12.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003899
    • TAT(Tat/SPI): 0.000204
    • LIPO(Sec/SPII): 0.000753
  • predicted transmembrane helices (TMHMM): 4

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTSFIYKILYVVKINAYTYDIMTEDIMILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLAITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILPNHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEKLMNI

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]