PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS02565 [old locus tag: SA0445 ]
• pan locus tag^?: SAUPAN002223000
• symbol: SA_RS02565
• pan gene symbol^?: —
• synonym:
• product: tRNA1(Val) (adenine(37)-N6)-methyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS02565 [old locus tag: SA0445 ]
• symbol: SA_RS02565
• product: tRNA1(Val) (adenine(37)-N6)-methyltransferase
• replicon: chromosome
• strand: +
• coordinates: 517096..517821
• length: 726
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (517096..517821) NCBI
• BioCyc: G1G21-498 BioCyc
• MicrobesOnline: see SA0445

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGTTAAAAGAGAATGAACGATTTGATCAACTAATCAAAGAAGATTTTAGTATTATTCAA
  AATGATGATGTTTTTTCGTTTTCAACGGATGCTTTGTTGTTAGGGCATTTTACAAAACCT
  AGAACAAAAGATATTGTGTTGGACTTATGTTCAGGCAATGGGGTGATACCCTTGTTATTG
  TTTGCGAAACATCCACGACATATAGAAGGTGTTGAGATTCAAAAAACACTTGTCGATATG
  GCGCGACGCACATTTCAATTCAATGATGTTGATGAATATTTAACAATGCATCACATGGAT
  TTGAAAAACGTTACTAAAGTATTTAAACCTTCACAATATACTTTAGTAACGTGTAATCCG
  CCTTATTTTAAAGAGAATCAGCAACACCAACATCAAAAAGAAGCACATAAGATAGCGAGA
  CATGAGATTATGTGTACACTTGAAGATTGCATGATTGCAGCCCGTCATTTATTAAAAGAA
  GGTGGCAGGCTAAATATGGTACATCGTGCAGATAGACTAATGGATGTCTTGTTTGAAATG
  AGAAAAGTGAATATTGAACCTAAGAAAGTCGTTTTTATATATAGTAAGGTAGGGAAATCA
  GCACAAACGATAGTAGTAGAAGGTCGAAAAGGTGGAAATCAAGGTTTAGAAATCATGCCC
  CCATTTTATATTTATAATGAAGATGGTAATTATAGCGAAGAAATGAAGGACGTATATTAT
  GGATAG
  60
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  726
  

⊟Protein

⊟General

• locus tag: SA_RS02565 [old locus tag: SA0445 ]
• symbol: SA_RS02565
• description: tRNA1(Val) (adenine(37)-N6)-methyltransferase
• length: 241
• theoretical pI: 7.10878
• theoretical MW: 28068.2
• GRAVY: -0.438589

⊟Function

• TIGRFAM:
  Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 45.6)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 37.5)
  and 7 more
  Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 36.3)
  Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 23.2)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 15.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 14.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 12.9)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification putative protein-(glutamine-N5) methyltransferase, unknown substrate-specific (TIGR03704; EC 2.1.1.-; HMM-score: 12.7)
  Metabolism Amino acid biosynthesis Aspartate family methionine biosynthesis protein MetW (TIGR02081; HMM-score: 11.8)
• TheSEED: see SA0445
• PFAM:
  NADP_Rossmann (CL0063) MTS; Methyltransferase small domain (PF05175; HMM-score: 56.4)
  and 12 more
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 33.5)
  Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 24.8)
  Cons_hypoth95; Conserved hypothetical protein 95 (PF03602; HMM-score: 20.5)
  UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 19.6)
  Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 18.4)
  Methyltransf_10; Protein of unknown function (DUF890) (PF05971; HMM-score: 18.3)
  Methyltransf_15; RNA cap guanine-N2 methyltransferase (PF09445; HMM-score: 18.2)
  N6-adenineMlase; Probable N6-adenine methyltransferase (PF10237; HMM-score: 17.2)
  Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 17.1)
  Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 14.9)
  PrmA; Ribosomal protein L11 methyltransferase (PrmA) (PF06325; HMM-score: 13.2)
  N6_N4_Mtase; DNA methylase (PF01555; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00241
  • TAT(Tat/SPI): 0.000678
  • LIPO(Sec/SPII): 0.000779
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446833430 NCBI
• RefSeq: WP_000910686 NCBI
• UniProt: see SA0445

⊟Protein sequence

• MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKDIVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYLTMHHMDLKNVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKEGGRLNMVHRADRLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEIMPPFYIYNEDGNYSEEMKDVYYG

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications