PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS03030 [old locus tag: SA0520 ]
• pan locus tag^?: SAUPAN002336000
• symbol: SA_RS03030
• pan gene symbol^?: sdrD
• synonym:
• product: serine-aspartate repeat-containing protein D

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS03030 [old locus tag: SA0520 ]
• symbol: SA_RS03030
• product: serine-aspartate repeat-containing protein D
• replicon: chromosome
• strand: +
• coordinates: 608443..612600
• length: 4158
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (608443..612600) NCBI
• BioCyc: SA_RS03030 BioCyc
• MicrobesOnline: see SA0520

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  1741
  1801
  1861
  1921
  1981
  2041
  2101
  2161
  2221
  2281
  2341
  2401
  2461
  2521
  2581
  2641
  2701
  2761
  2821
  2881
  2941
  3001
  3061
  3121
  3181
  3241
  3301
  3361
  3421
  3481
  3541
  3601
  3661
  3721
  3781
  3841
  3901
  3961
  4021
  4081
  4141
  
  ATGCTAAACAGAGAAAATAAAACGGCAATAACAAGAAAAGGCATGGTATCCAATCGATTA
  AATAAATTTTCGATTAGAAAGTACACAGTGGGAACAGCATCAATTTTAGTAGGTACAACA
  TTAATTTTTGGTCTGGGGAACCAAGAAGCAAAGGCTGCAGAAAGTACTAATAAAGAATTG
  AACGAAGCGACAACTTCAGCAAGTGATAATCAATCGAGTGATAAAGTTGATATGCAGCAA
  CTAAATCAAGAAGACAATACTAAAAATGATAATCAAAAAGAAATGGTATCATCTCAAGGT
  AATGAAACGACTTCAAATGGGAATAAATCAATAGAAAAAGAAAGTGTACAATCTACCACT
  GGAAATAAAGTTGAAGTTTCAACTGCCAAATCAGATGAGCAAGCTTCACCAAAATCTACG
  AATGAAGATTTAAACACTAAACAAACTATAAGTAATCAAGAAGGGTTACAACCTGATTTG
  CTAGAGAATAAATCAGTGGTAAATGTTCAACCAACTAATGAGGAAAACAAAAAGGTAGAT
  GCGAAAACTGAATCAACTACATTAAATGTTAAAAGTGATGCTATCAAGAGTAATGCTGAA
  ACTCTTGTTGATAACAATAGTAATTCAAATAATGAAAATAATGCAGATATCATTTTGCCA
  AAAAGTACAGCACCTAAAAGTTTGAATACAAGAATGCGTATGGCAGCAATACAACCAAAC
  TCAACAGATTCTAAAAATGTTAATGATTTAATCACATCAAATACAACATTAACTGTCGTT
  GATGCAGATAATAGCAAAACGATTGTACCAGCCCAAGATTATTTATCATTAAAATCACAA
  ATTACAGTTGATGACAAAGTTAAATCAGGTGATTATTTCACAATTAAATACTCAGATACA
  GTACAAGTATATGGATTGAATCCGGAAGATATTAAAAATATTGGTGATATTAAAGATCCA
  AATAATGGTGAAACAATTGCGACTGCAAAACATGATACTGCAAATAATTTAATTACATAT
  ACATTTACAGATTATGTTGATCGATTTAATTCAGTAAAAATGGGTATTAATTACTCAATT
  TATATGGATGCAGATACAATTCCTGTTGACAAGAAAGATGTTCCTTTTAGTGTAACTATT
  GGAAATCAAATTACAACTACAACAGCAGATATCACTTATCCGGCTTATAAAGAAGCTGAC
  AATAATTCAATAGGATCAGCTTTTACAGAGACAGTTTCTCATGTAGGAAATGTTGAAGAC
  CCTGGTTACTATAACCAGGTAGTATATGTTAATCCTATGGATAAGGATTTAAAAGGTGCT
  AAGTTAAAAGTTGAAGCGTACCATCCGAAATATCCAACTAATATTGGTCAAATTAATCAA
  AATGTTACAAATATAAAAATATATCGTGTTCCTGAAGGATATACATTGAATAAAGGATAT
  GACGTTAATACTAATGATTTGGTAGACGTAACTGATGAATTTAAAAATAAAATGACGTAT
  GGATCAAATCAAAGTGTTAATCTTGATTTTGGTGATATTACATCAGCATATGTTGTAATG
  GTTAATACAAAATTCCAATATACAAATAGCGAAAGCCCAACACTTGTTCAAATGGCTACT
  TTATCTTCAACAGGTAATAAATCCGTTTCTACTGGCAATGCTTTAGGATTTACTAATAAC
  CAAAGTGGCGGAGCTGGTCAAGAAGTATATAAAATTGGTAACTACGTATGGGAAGATACT
  AATAAAAACGGTGTTCAAGAATTAGGAGAAAAAGGCGTTGGCAATGTAACTGTAACTGTA
  TTTGATAATAATACAAATACAAAAGTAGGAGAAGCAGTTACTAAAGAAGATGGGTCATAC
  TTGATTCCAAACTTACCTAATGGAGATTACCGTGTAGAATTTTCAAACTTACCAAAAGGT
  TATGAAGTAACCCCTTCAAAACAAGGTAATAACGAAGAATTAGATTCAAACGGCTTATCT
  TCAGTTATTACAGTTAATGGCAAAGATAACTTATCTGCAGACTTAGGTATTTACAAACCT
  AAATACAACTTAGGTGACTATGTCTGGGAAGATACAAATAAAAATGGTATCCAAGACCAA
  GATGAAAAAGGTATATCTGGCGTAACGGTAACATTAAAAGATGAAAACGGTAACGTGTTA
  AAAACAGTTACAACAGACGCAGATGGCAAATATAAATTTACTGATTTAGATAATGGTAAT
  TATAAAGTTGAATTTACTACACCAGAAGGCTATACACCGACTACAGTAACATCTGGTAGC
  GACATTGAAAAAGACTCTAATGGTTTAACAACAACAGGTGTTATTAATGGTGCTGATAAC
  ATGACATTAGATAGTGGATTCTACAAAACACCAAAATATAATTTAGGTAATTATGTATGG
  GAAGATACAAATAAAGATGGTAAGCAGGATTCAACTGAAAAAGGTATTTCAGGCGTAACA
  GTTACATTGAAAAATGAAAACGGTGAAGTTTTACAAACAACTAAAACAGATAAAGATGGT
  AAATATCAATTTACTGGATTAGAAAATGGAACTTATAAAGTTGAATTCGAAACACCATCA
  GGTTACACACCAACACAAGTAGGTTCAGGAACTGATGAAGGTATAGATTCAAATGGTACA
  TCAACAACAGGTGTCATTAAAGATAAAGATAACGATACTATTGACTCTGGTTTCTACAAA
  CCGACTTACAACTTAGGTGACTATGTATGGGAAGATACAAATAAAAACGGTGTTCAAGAT
  AAAGATGAAAAGGGTATTTCAGGTGTAACAGTTACGTTAAAAGATGAAAACGACAAAGTT
  TTAAAAACAGTTACAACAGATGAAAATGGTAAATATCAATTCACTGATTTAAACAATGGA
  ACTTATAAAGTTGAATTCGAGACACCATCAGGTTATACACCAACTTCAGTAACTTCTGGA
  AATGATACTGAAAAAGATTCTAATGGTTTAACAACAACAGGTGTCATTAAAGATGCAGAT
  AACATGACATTAGACAGTGGTTTCTATAAAACACCAAAATATAGTTTAGGTGATTATGTT
  TGGTACGACAGTAATAAAGACGGCAAACAAGATTCAACTGAAAAAGGTATCAAAGATGTT
  AAAGTTATTTTATTAAATGAAAAAGGCGAAGTAATTGGAACAACTAAAACAGATGAAAAT
  GGTAAATACCGCTTTGATAATTTAGATAGCGGTAAATACAAAGTTATTTTTGAAAAGCCT
  ACTGGCTTAACACAAACAGGTACAAATACAACTGAAGATGATAAAGATGCCGATGGTGGC
  GAAGTTGATGTAACAATTACGGATCATGATGATTTCACACTTGATAATGGCTACTACGAA
  GAAGAAACATCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGACAGCGATTCA
  GACTCAGATAGTGATTCAGATTCAGATAGTGATTCAGATTCAGATAGTGATTCAGATTCA
  GACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGATTCAGATTCAGATAGC
  GATTCAGACTCAGACAGCGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCA
  GATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCA
  GATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGACAGT
  GATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACTCAGATAGC
  GATTCAGATTCAGACAGCGACTCAGATTCGGACAGCGACTCAGACTCAGACAGTGATTCA
  GATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCA
  GATAGTGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGT
  GATTCAGACTCAGATGCAGGTAAGCACACACCTGTTAAACCAATGAGTACTACTAAAGAC
  CATCACAATAAAGCAAAAGCATTACCAGAAACAGGTAATGAAAATAGTGGCTCAAATAAC
  GCAACGTTATTTGGCGGATTATTCGCAGCATTAGGATCATTATTGTTATTCGGTCGTCGT
  AAAAAACAAAATAAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1680
  1740
  1800
  1860
  1920
  1980
  2040
  2100
  2160
  2220
  2280
  2340
  2400
  2460
  2520
  2580
  2640
  2700
  2760
  2820
  2880
  2940
  3000
  3060
  3120
  3180
  3240
  3300
  3360
  3420
  3480
  3540
  3600
  3660
  3720
  3780
  3840
  3900
  3960
  4020
  4080
  4140
  4158
  

⊟Protein

⊟General

• locus tag: SA_RS03030 [old locus tag: SA0520 ]
• symbol: SA_RS03030
• description: serine-aspartate repeat-containing protein D
• length: 1385
• theoretical pI: 3.89339
• theoretical MW: 149643
• GRAVY: -1.02686

⊟Function

• TIGRFAM:
  gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 49)
  and 3 more
  Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 18.6)
  Metabolism Energy metabolism Other protocatechuate 3,4-dioxygenase, beta subunit (TIGR02422; EC 1.13.11.3; HMM-score: 18.6)
  Por secretion system C-terminal sorting domain (TIGR04183; HMM-score: 8.8)
• TheSEED: see SA0520
• PFAM:
  Transthyretin (CL0287) SdrD_B; SdrD B-like domain (PF17210; HMM-score: 465.1)
  and 17 more
  CarboxypepD_reg; Carboxypeptidase regulatory-like domain (PF13620; HMM-score: 174)
  Adhesin (CL0204) SdrG_C_C; C-terminus of bacterial fibrinogen-binding adhesin (PF10425; HMM-score: 114)
  E-set (CL0159) MG1; Alpha-2-macroglobulin MG1 domain (PF11974; HMM-score: 76.6)
  Transthyretin (CL0287) CarbopepD_reg_2; CarboxypepD_reg-like domain (PF13715; HMM-score: 75.6)
  MetallophosN; N terminal of Calcineurin-like phosphoesterase (PF16371; HMM-score: 57.5)
  DUF2012; Protein of unknown function (DUF2012) (PF09430; HMM-score: 57.4)
  no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 45.4)
  Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 42.7)
  E-set (CL0159) DUF3244; Domain of unknown function (DUF3244) (PF11589; HMM-score: 41.8)
  Transthyretin (CL0287) DUF1416; Protein of unknown function (DUF1416) (PF07210; HMM-score: 37.3)
  Dioxygenase_C; Dioxygenase (PF00775; HMM-score: 27.1)
  TTR-52; Transthyretin-like family (PF01060; HMM-score: 26)
  GOLD-like (CL0521) EMP24_GP25L; emp24/gp25L/p24 family/GOLD (PF01105; HMM-score: 24.4)
  bBprotInhib (CL0354) Inh; Protease inhibitor Inh (PF02974; HMM-score: 12.9)
  Transthyretin (CL0287) DUF2606; Protein of unknown function (DUF2606) (PF10794; HMM-score: 11.8)
  HSP20 (CL0190) HSP20; Hsp20/alpha crystallin family (PF00011; HMM-score: 11)
  Transthyretin (CL0287) fn3_3; Polysaccharide lyase family 4, domain II (PF14686; HMM-score: 8.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 10
  • Extracellular Score: 0
  • Internal Helix: 1
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 52 aa
  • SP(Sec/SPI): 0.812615
  • TAT(Tat/SPI): 0.093189
  • LIPO(Sec/SPII): 0.002787
  • Cleavage Site: CS pos: 52-53. AKA-AE. Pr: 0.8678
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 446857211 NCBI
• RefSeq: WP_000934467 NCBI
• UniProt: see SA0520

⊟Protein sequence

• MLNRENKTAITRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFGLGNQEAKAAESTNKELNEATTSASDNQSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNETTSNGNKSIEKESVQSTTGNKVEVSTAKSDEQASPKSTNEDLNTKQTISNQEGLQPDLLENKSVVNVQPTNEENKKVDAKTESTTLNVKSDAIKSNAETLVDNNSNSNNENNADIILPKSTAPKSLNTRMRMAAIQPNSTDSKNVNDLITSNTTLTVVDADNSKTIVPAQDYLSLKSQITVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTANNLITYTFTDYVDRFNSVKMGINYSIYMDADTIPVDKKDVPFSVTIGNQITTTTADITYPAYKEADNNSIGSAFTETVSHVGNVEDPGYYNQVVYVNPMDKDLKGAKLKVEAYHPKYPTNIGQINQNVTNIKIYRVPEGYTLNKGYDVNTNDLVDVTDEFKNKMTYGSNQSVNLDFGDITSAYVVMVNTKFQYTNSESPTLVQMATLSSTGNKSVSTGNALGFTNNQSGGAGQEVYKIGNYVWEDTNKNGVQELGEKGVGNVTVTVFDNNTNTKVGEAVTKEDGSYLIPNLPNGDYRVEFSNLPKGYEVTPSKQGNNEELDSNGLSSVITVNGKDNLSADLGIYKPKYNLGDYVWEDTNKNGIQDQDEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGNYKVEFTTPEGYTPTTVTSGSDIEKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNYVWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEFETPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTNKNGVQDKDEKGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGYTPTSVTSGNDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKDVKVILLNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPTGLTQTGTNTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKHTPVKPMSTTKDHHNKAKALPETGNENSGSNNATLFGGLFAALGSLLLFGRRKKQNK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications