PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS03035 [old locus tag: SA0521 ]
• pan locus tag^?: SAUPAN002337000
• symbol: SA_RS03035
• pan gene symbol^?: sdrE
• synonym:
• product: MSCRAMM family adhesin SdrE

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS03035 [old locus tag: SA0521 ]
• symbol: SA_RS03035
• product: MSCRAMM family adhesin SdrE
• replicon: chromosome
• strand: +
• coordinates: 612994..616419
• length: 3426
• essential: no DEG

⊟Accession numbers

• Location: NC_002745 (612994..616419) NCBI
• BioCyc: SA_RS03035 BioCyc
• MicrobesOnline: see SA0521

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGATTAACAGGGATAATAAAAAGGCAATAACAAAAAAGGGTATGATTTCAAATCGCTTA
  AACAAATTTTCGATTAGAAAGTATACTGTAGGAACTGCATCGATTTTAGTAGGTACGACA
  TTGATTTTTGGTCTAGGGAACCAAGAAGCTAAAGCTGCTGAAAACACTAGTACAGAAAAT
  GCGAAACAAGATGATGCAACGACTAGTGATAATAAAGAAGTAGTGTCGGAAACTGAAAAT
  AATTCGACAACAGAAAATGATTCAACAAATCCAATTAAGAAAGAAACAAATACTGATTCA
  CAACCAGAAGCTAAAGAAGAATCAACTACATCAAGTACTCAACAACAGCAAAATAACGTT
  ACAGCTACAACTGAAACTAAGCCTCAAAACATTGAAAAAGAAAATGTTAAACCTTCAACT
  GATAAAACTGCGACAGAAGATACATCTGTTATTTTAGAAGAGAAGAAAGCACCAAATTAT
  ACAAATAACGATGTAACTACAAAACCATCTACAAGTGAAATTCAAACAAAACCAACTACA
  CCTCAAGAATCTACAAATATTGAAAATTCACAACCGCAACCAACGCCTTCAAAAGTAGAC
  AATCAAGTTACAGATGCAACTAATCCAAAAGAACCAGTAAATGTGTCAAAAGAAGAACTT
  AAAAATAATCCTGAGAAATTAAAAGAATTAGTTAGAAATGATAACAATACAGATCGTTCA
  ACTAAACCAGTTGCTACAGCTCCAACAAGTGTTGCACCAAAACGATTAAATGCGAAAATG
  CGTTTTGCAGTTGCACAACCAGCAGCAGTTGCTTCAAATAATGTAAATGACTTAATTACA
  GTTACGAAACAGACGATCAAAGTTGGCGATGGTAAAGATAATGTGGCAGCAGCGCATGAC
  GGTAAAGATATTGAATATGATACAGAGTTTACAATTGACAATAAAGTCAAAAAAGGCGAT
  ACAATGACGATTAATTATGATAAGAATGTAATTCCTTCGGATTTAACAGATAAAAATGAT
  CCTATCGATATTACTGATCCATCAGGAGAGGTCATTGCCAAAGGAACATTTGATAAAGCG
  ACTAAGCAAATCACATATACATTTACAGATTATGTAGATAAATATGAAGATATAAAAGCA
  CGTTTAACTTTATACTCATATATTGATAAGCAAGCAGTACCTAATGAAACTAGTTTGAAT
  TTAACGTTTGCAACAGCAGGTAAAGAAACTAGCCAAAACGTTTCTGTTGATTATCAAGAC
  CCAATGGTTCATGGTGATTCAAACATTCAATCTATCTTTACAAAGTTAGATGAAAACAAA
  CAAACTATTGAACAACAAATTTATGTTAATCCTTTGAAAAAAACAGCAACTAACACTAAA
  GTTGATATAGCTGGTAGTCAAGTAGATGATTATGGAAATATTAAACTAGGAAATGGTAGT
  ACCATTATTGACCAAAATACAGAAATAAAAGTTTATAAAGTTAACCCTAATCAACAATTG
  CCTCAAAGTAATAGAATCTATGATTTTAGTCAATACGAAGATGTAACAAGTCAATTTGAT
  AATAAAAAATCATTTAGTAATAATGTAGCAACATTGGATTTTGGTGATATTAATTCAGCC
  TATATTATCAAAGTTGTTAGTAAATATACACCTACATCAGATGGCGAACTAGATATTGCT
  CAAGGTACTAGTATGAGAACAACTGATAAATATGGTTATTATAATTATGCAGGATATTCA
  AACTTCATCGTAACTTCTAATGACACTGGCGGTGGCGACGGTACTGTTAAACCTGAAGAA
  AAGTTATACAAAATTGGTGACTATGTATGGGAAGACGTTGATAAAGACGGTGTCCAAGGT
  ACAGATTCGAAAGAAAAGCCAATGGCAAACGTTTTAGTTACATTAACTTACCCGGACGGT
  ACTACAAAATCAGTAAGAACAGATGCTAACGGTCATTATGAATTCGGTGGTTTGAAAGAC
  GGAGAAACTTATACAGTTAAATTCGAAACGCCAGCTGGATATCTTCCAACAAAAGTAAAT
  GGAACAACTGATGGTGAAAAAGACTCAAATGGTAGTTCTATAACTGTTAAAATTAATGGT
  AAAGATGATATGTCTTTAGACACTGGTTTTTATAAAGAACCTAAATATAATCTTGGTGAC
  TATGTATGGGAAGATACAAATAAAGATGGTATCCAAGATGCTAATGAACCTGGTATCAAA
  GATGTTAAGGTTACATTAAAAGATAGTACTGGAAAAGTTATTGGTACAACTACTACTGAT
  GCCTCGGGTAAATATAAATTTACAGATTTAGATAATGGTAACTATACAGTAGAATTTGAA
  ACACCAGCAGGTTACACGCCAACGGTTAAAAATACTACAGCTGAAGATAAAGATTCTAAT
  GGTTTAACAACAACAGGTGTCATTAAAGATGCAGATAATATGACATTAGACAGTGGTTTC
  TATAAAACACCAAAATACAGTTTAGGTGATTATGTTTGGTACGACAGTAATAAAGACGGT
  AAACAAGATTCAACTGAAAAAGGTATCAAAGATGTTAAAGTTACTTTATTAAATGAAAAA
  GGCGAAGTAATTGGAACAACTAAAACAGATGAAAATGGTAAATATCGTTTCGATAATTTA
  GATAGCGGTAAATACAAAGTTATTTTTGAAAAGCCTGCTGGCTTAACACAAACAGTTACA
  AATACAACTGAAGATGATAAAGATGCCGATGGTGGCGAAGTTGACGTAACAATTACGGAT
  CATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGTGATTCA
  GACTCAGACAGTGATTCAGACTCAGACAGCGACTCAGATTCAGACAGTGATTCAGACTCA
  GATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGC
  GACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCA
  GATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCA
  GATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGATTCAGATTCAGATAGC
  GACTCAGATTCGGACAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCA
  GACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGACTCAGATAGCGATTCGGACTCG
  GATGCAGGAAAACATACACCTGTTAAACCAATGAGTACTACTAAAGACCATCACAATAAA
  GCAAAAGCATTACCAGAAACAGGTAGTGAAAATAACGGCTCAAATAACGCAACGTTATTT
  GGTGGATTATTTGCAGCATTAGGTTCATTATTGTTATTCGGTCGTCGCAAAAAACAAAAC
  AAATAA
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  3426
  

⊟Protein

⊟General

• locus tag: SA_RS03035 [old locus tag: SA0521 ]
• symbol: SA_RS03035
• description: MSCRAMM family adhesin SdrE
• length: 1141
• theoretical pI: 4.03874
• theoretical MW: 124026
• GRAVY: -1.06705

⊟Function

• TIGRFAM:
  gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 50.2)
  and 3 more
  Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 16.4)
  Por secretion system C-terminal sorting domain (TIGR04183; HMM-score: 16.3)
  Metabolism Energy metabolism Other protocatechuate 3,4-dioxygenase, beta subunit (TIGR02422; EC 1.13.11.3; HMM-score: 12.4)
• TheSEED: see SA0521
• PFAM:
  Transthyretin (CL0287) SdrD_B; SdrD B-like domain (PF17210; HMM-score: 250.6)
  and 12 more
  CarboxypepD_reg; Carboxypeptidase regulatory-like domain (PF13620; HMM-score: 105.1)
  Adhesin (CL0204) SdrG_C_C; C-terminus of bacterial fibrinogen-binding adhesin (PF10425; HMM-score: 102.3)
  E-set (CL0159) MG1; Alpha-2-macroglobulin MG1 domain (PF11974; HMM-score: 48.5)
  no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 45.4)
  Transthyretin (CL0287) MetallophosN; N terminal of Calcineurin-like phosphoesterase (PF16371; HMM-score: 44.1)
  no clan defined Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 42.7)
  Transthyretin (CL0287) CarbopepD_reg_2; CarboxypepD_reg-like domain (PF13715; HMM-score: 29.3)
  DUF2012; Protein of unknown function (DUF2012) (PF09430; HMM-score: 20.4)
  DUF2606; Protein of unknown function (DUF2606) (PF10794; HMM-score: 18.1)
  DUF1416; Protein of unknown function (DUF1416) (PF07210; HMM-score: 17.7)
  no clan defined CompInhib_SCIN; Staphylococcal complement inhibitor SCIN (PF11546; HMM-score: 16.8)
  GOLD-like (CL0521) EMP24_GP25L; emp24/gp25L/p24 family/GOLD (PF01105; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 10
  • Extracellular Score: 0
  • Internal Helix: 1
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 52 aa
  • SP(Sec/SPI): 0.83357
  • TAT(Tat/SPI): 0.075298
  • LIPO(Sec/SPII): 0.002832
  • Cleavage Site: CS pos: 52-53. AKA-AE. Pr: 0.8729
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI: 446532913 NCBI
• RefSeq: WP_000610259 NCBI
• UniProt: see SA0521

⊟Protein sequence

• MINRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFGLGNQEAKAAENTSTENAKQDDATTSDNKEVVSETENNSTTENDSTNPIKKETNTDSQPEAKEESTTSSTQQQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNYTNNDVTTKPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPVNVSKEELKNNPEKLKELVRNDNNTDRSTKPVATAPTSVAPKRLNAKMRFAVAQPAAVASNNVNDLITVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKNVIPSDLTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKARLTLYSYIDKQAVPNETSLNLTFATAGKETSQNVSVDYQDPMVHGDSNIQSIFTKLDENKQTIEQQIYVNPLKKTATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNPNQQLPQSNRIYDFSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQGTSMRTTDKYGYYNYAGYSNFIVTSNDTGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQGTDSKEKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPAGYLPTKVNGTTDGEKDSNGSSITVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDANEPGIKDVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTTAEDKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKDVKVTLLNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDADGGEVDVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKHTPVKPMSTTKDHHNKAKALPETGSENNGSNNATLFGGLFAALGSLLLFGRRKKQNK

⊟Experimental data

• experimentally validated: data available for COL
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications