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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS03080 [old locus tag: SA0530 ]
  • pan locus tag?: SAUPAN002349000
  • symbol: SA_RS03080
  • pan gene symbol?: —
  • synonym:
  • product: HAD family hydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS03080 [old locus tag: SA0530 ]
  • symbol: SA_RS03080
  • product: HAD family hydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 624247..624894
  • length: 648
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (624247..624894) NCBI
  • BioCyc: SA_RS03080 BioCyc
  • MicrobesOnline: see SA0530

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    TTGAAGTTTGACAATTATATTTTTGATTTTGATGGTACGTTGGCAGACACGAAAAAATGT
    GGTGAAGTAGCAACACAAAGTGCATTTAAAGCATGTGGCTTAACGGAACCATCATCTAAA
    GAAATAACGCATTATATGGGAATACCTATTGAAGAATCATTTTTAAAATTAGCAGACCGA
    CCATTAGATGAAGCAGCATTAGCAAAGTTAATCGATACATTTAGACATACATATCAATCT
    ATTGAAAAGGACTATATTTATGAATTTGCGGGTATAACTGAAGCCATTACAAGTTTGTAT
    AACCAAGGGAAAAAACTTTTCGTGGTGTCTAGTAAGAAGAGTGATGTATTAGAAAGAAAT
    TTATCGGCTATTGGATTAAATCACTTGATTACCGAAGCTGTTGGATCCGATCAAGTAAGT
    GCATATAAACCAAATCCTGAAGGCATACACACAATTGTGCAACGCTACAATTTAAATAGC
    CAACAAACGGTTTATATTGGTGATTCAACATTTGATGTTGAGATGGCGCAACATGCTGGT
    GTACCATCTGCAGCAGTAACATGGGGAGCACATGATGCAAGGTCATTACTTCATTCAAAT
    CCAGATTTTATAATCAATGATCCATCAGAAATTAATACAGTATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    648

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS03080 [old locus tag: SA0530 ]
  • symbol: SA_RS03080
  • description: HAD family hydrolase
  • length: 215
  • theoretical pI: 4.79139
  • theoretical MW: 23784.5
  • GRAVY: -0.236279

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Energy metabolism Sugars phosphoglycolate phosphatase, bacterial (TIGR01449; EC 3.1.3.18; HMM-score: 95.4)
    AHBA synthesis associated protein (TIGR01454; HMM-score: 79.7)
    and 34 more
    phosphonatase-like hydrolase (TIGR03351; HMM-score: 64.5)
    HAD hydrolase, TIGR02253 family (TIGR02253; HMM-score: 64.3)
    beta-phosphoglucomutase family hydrolase (TIGR02009; HMM-score: 53.4)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIIA (TIGR01662; HMM-score: 48.4)
    HAD hydrolase, REG-2-like, family IA (TIGR02252; HMM-score: 45.4)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 1 (TIGR01549; HMM-score: 44.2)
    haloacid dehalogenase, type II (TIGR01428; EC 3.8.1.2; HMM-score: 42.3)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides beta-phosphoglucomutase (TIGR01990; EC 5.4.2.6; HMM-score: 38.7)
    noncanonical pyrimidine nucleotidase, YjjG family (TIGR02254; EC 3.1.3.5; HMM-score: 37.4)
    HAD hydrolase, TIGR01548 family (TIGR01548; HMM-score: 32.9)
    Metabolism Central intermediary metabolism Other phosphonoacetaldehyde hydrolase (TIGR01422; EC 3.11.1.1; HMM-score: 29.9)
    Metabolism Amino acid biosynthesis Serine family phosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 28.9)
    Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 28.4)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 3 (TIGR01509; HMM-score: 28.3)
    histidinol-phosphate phosphatase domain (TIGR01656; HMM-score: 28.1)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family (TIGR01670; EC 3.1.3.45; HMM-score: 26.9)
    HAD phosphatase, family IIIC (TIGR01681; HMM-score: 25.2)
    Unknown function Enzymes of unknown specificity HAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 23.3)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIB (TIGR01484; HMM-score: 22.8)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IB (TIGR01490; HMM-score: 20.7)
    pyrimidine 5'-nucleotidase (TIGR01993; EC 3.1.3.5; HMM-score: 19.3)
    HAD phosphatase, family IIIA (TIGR01668; EC 3.1.3.-; HMM-score: 18.9)
    FkbH domain (TIGR01686; HMM-score: 18.8)
    phosphoglycolate/pyridoxal phosphate phosphatase family (TIGR01452; EC 3.1.3.18; HMM-score: 18.7)
    phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 18.5)
    DNA 3'-phosphatase (TIGR01664; EC 3.1.3.32; HMM-score: 18.2)
    Metabolism Central intermediary metabolism Other 2,3-diketo-5-methylthio-1-phosphopentane phosphatase (TIGR01489; HMM-score: 17)
    phosphoserine phosphatase-like hydrolase, archaeal (TIGR01491; HMM-score: 15.5)
    Unknown function Enzymes of unknown specificity HAD hydrolase, TIGR01457 family (TIGR01457; HMM-score: 14.5)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIA (TIGR01460; HMM-score: 14.1)
    HAD hydrolase, family IA, variant 2 (TIGR01493; HMM-score: 13.1)
    sucrose-phosphate phosphatase subfamily (TIGR01482; EC 3.1.3.-; HMM-score: 12.3)
    Metabolism Amino acid biosynthesis Histidine family histidinol-phosphatase (TIGR01261; EC 3.1.3.15; HMM-score: 12.1)
    epoxide hydrolase N-terminal domain-like phosphatase (TIGR02247; HMM-score: 11.9)
  • TheSEED: see SA0530
  • PFAM:
    HAD (CL0137) HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 150.7)
    and 5 more
    Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 51)
    Hydrolase_like; HAD-hyrolase-like (PF13242; HMM-score: 45.4)
    HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 37.9)
    Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 30)
    PNK3P; Polynucleotide kinase 3 phosphatase (PF08645; HMM-score: 15)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012731
    • TAT(Tat/SPI): 0.000669
    • LIPO(Sec/SPII): 0.010902
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKFDNYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSSKEITHYMGIPIEESFLKLADRPLDEAALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQGKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNSQQTVYIGDSTFDVEMAQHAGVPSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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