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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA_RS03090 [old locus tag: SA0533 ]
- pan locus tag?: SAUPAN002352000
- symbol: SA_RS03090
- pan gene symbol?: vraA
- synonym:
- product: long-chain-fatty-acid--CoA ligase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1321ATGAACGTAATTTTAGAACAGTTGAAAACACATACTCAAAATAAACCTAATGACATAGCA
TTACATATCGATGATGAAACAATTACATATAGTCAACTAAATGCCCGCATCACTAGCGCA
GTTGAATCTTTGCAGAAATATTCACTTAACCCTGTCGTTGCTATTAATATGAAATCACCG
GTGCAAAGTATTATTTGTTATTTAGCTTTGCATCGTTTACATAAAGTGCCTATGATGATG
GAAGGTAAATGGCAAAGTACTATACATCGTCAATTGATTGAAAAATATGGTATTAAAGAT
GTAATTGGAGATACAGGTCTCATGCAGAATATAGACTCACCGATGTTTATTGATTCAACG
CAATTACAGCACTACCCCAATTTATTACATATTGGTTTTACTTCAGGGACAACTGGACTG
CCAAAAGCATATTATCGTGATGAAGATTCATGGTTGGCTTCTTTTGAAGTTAATGAAATG
TTGATGTTAAAAAATGAAAATGCAATAGCAGCCCCTGGACCACTATCGCACTCGTTAACA
TTATATGCGTTATTGTTTGCTTTAAGTTCCGGTCGTACTTTTATAGGACAGACCACTTTT
CATCCTGAAAAGTTACTTAATCAATGTCATAAAATATCATCATACAAAGTTGCTATGTTT
CTTGTTCCAACGATGATTAAATCATTATTGTTAGTTTACAACAATGAACATACAATCCAA
TCATTTTTTAGCAGTGGAGATAAGCTGCATTCTTCTATTTTTAAAAAGATAAAAAATCAA
GCAAATGACATAAATTTGATTGAATTTTTTGGTACATCGGAAACCAGTTTTATCAGCTAT
AACTTGAATCAGCAAGCACCAGTTGAATCAGTAGGTGTGCTATTTCCAAATGTGGAATTG
AAAACAACGAATCACGATCACAATGGTATAGGAACTATTTGTATAAAAAGTAATATGATG
TTTAGTGGCTATGTAAGTGAACAATGTATAAATAATGATGAATGGTTTGTTACTAATGAT
AATGGCTATGTAAAAGAGCAGTATTTATATTTAACGGGACGTCAACAGGATATGTTAATT
ATTGGTGGTCAAAATATATATCCAGCACATGTTGAACGCCTTTTAACGCAATCTTCGAGC
ATTGATGAAGCAATTATCATCGGTATTCCAAATGAGCGTTTTGGTCAAATAGGCGTATTG
CTTTATTCTGGTGATGTGACACTTACACATAAAAATGTAAAACAATTTTTAAAAAAGAAA
GTGAAACGCTATGAAATTCCATCGATGATTCATCATGTAGAAAAGATGTATTACACTGCA
AGTGGTAAAATTGCTAGAGAAAAAATGATGTCGATGTATTTGAGAGGTGAATTATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA_RS03090 [old locus tag: SA0533 ]
- symbol: SA_RS03090
- description: long-chain-fatty-acid--CoA ligase
- length: 458
- theoretical pI: 7.53263
- theoretical MW: 52023.6
- GRAVY: -0.186245
⊟Function[edit | edit source]
- reaction: EC 6.2.1.-? ExPASy
- TIGRFAM: Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone O-succinylbenzoate-CoA ligase (TIGR01923; EC 6.2.1.26; HMM-score: 171.1)and 13 moreacyl-CoA ligase (AMP-forming), exosortase A-associated (TIGR03098; HMM-score: 85.6)amino acid adenylation domain (TIGR01733; HMM-score: 78.7)cyclohexanecarboxylate-CoA ligase (TIGR03208; EC 6.2.1.-; HMM-score: 75.7)dicarboxylate--CoA ligase PimA (TIGR03205; EC 6.2.1.23; HMM-score: 69.1)Transport and binding proteins Cations and iron carrying compounds (2,3-dihydroxybenzoyl)adenylate synthase (TIGR02275; EC 2.7.7.58; HMM-score: 52.2)Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan D-alanine--poly(phosphoribitol) ligase, subunit 1 (TIGR01734; EC 6.1.1.13; HMM-score: 51.8)acetate--CoA ligase (TIGR02188; EC 6.2.1.1; HMM-score: 47.4)benzoate-CoA ligase family (TIGR02262; HMM-score: 46.8)propionate--CoA ligase (TIGR02316; EC 6.2.1.17; HMM-score: 22.9)Energy metabolism Other phenylacetate-CoA ligase (TIGR02155; EC 6.2.1.30; HMM-score: 19.5)4-coumarate--CoA ligase (TIGR02372; EC 6.2.1.12; HMM-score: 17.3)L-aminoadipate-semialdehyde dehydrogenase (TIGR03443; EC 1.2.1.31; HMM-score: 15.8)Central intermediary metabolism Other acetoacetate-CoA ligase (TIGR01217; EC 6.2.1.16; HMM-score: 11.3)
- TheSEED: see SA0533
- PFAM: ANL (CL0378) AMP-binding; AMP-binding enzyme (PF00501; HMM-score: 118.5)and 2 moreAMP-binding_C (CL0531) AMP-binding_C; AMP-binding enzyme C-terminal domain (PF13193; HMM-score: 27.7)POZ (CL0033) BTB; BTB/POZ domain (PF00651; HMM-score: 15.2)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.005925
- TAT(Tat/SPI): 0.000246
- LIPO(Sec/SPII): 0.000672
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESLQKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVIGDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEMLMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVAMFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETSFISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDEWFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNERFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMMSMYLRGEL
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: BirA* see SA0533
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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