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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS03140 [old locus tag: SA0542 ]
  • pan locus tag?: SAUPAN002363000
  • symbol: SA_RS03140
  • pan gene symbol?:
  • synonym:
  • product: membrane protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS03140 [old locus tag: SA0542 ]
  • symbol: SA_RS03140
  • product: membrane protein
  • replicon: chromosome
  • strand: -
  • coordinates: 635642..636094
  • length: 453
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (635642..636094) NCBI
  • BioCyc: SA_RS03140 BioCyc
  • MicrobesOnline: see SA0542

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGTTTTGGATCTTAAACTTTATCTTTAGCTTTTTAGCTTCAATGTTCTTCTGCGTCATT
    TTTGATGCACCTAGAAAATTATATCTATCATGTGGTTTCGTTGGTACATGTGGATGGATG
    GTTTACACCTTATTCTTCAACGGCTTTAATGTGCACACTATATACTCTAGTTTCTTTGGT
    AGTTTAGCATTAGGCTTGTTAAGTCATTATATGGCTCGTAAACAAAAAGAACCTGCCATC
    ATTTTTATGGTAACGGGTATCATTCCATTAGTACCAGGTGGATTAGCATACGATGCTACA
    AAAAATTTAGTCTTATTAAATTTCAGCACAGCAATCAATACCATGTTAGAAGTAACACTT
    ATTGCAGGCGCCATCGCATTAGGTTTATTATTCGCCGACCAAATTTCCAAATTAATTGTT
    TCTGGGTTCGTGAAATCTTTTAAACGATTATAA
    60
    120
    180
    240
    300
    360
    420
    453

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS03140 [old locus tag: SA0542 ]
  • symbol: SA_RS03140
  • description: membrane protein
  • length: 150
  • theoretical pI: 9.5134
  • theoretical MW: 16667.9
  • GRAVY: 0.964

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: see SA0542
  • PFAM:
    UMP_1 (CL0470) ThrE_2; Threonine/Serine exporter, ThrE (PF12821; HMM-score: 134)
    and 3 more
    ThrE; Putative threonine/serine exporter (PF06738; HMM-score: 34.1)
    no clan defined Flp_Fap; Flp/Fap pilin component (PF04964; HMM-score: 16.4)
    GPCR_A (CL0192) 7TMR-DISM_7TM; 7TM diverse intracellular signalling (PF07695; HMM-score: 12.8)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 5
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.041446
    • TAT(Tat/SPI): 0.001105
    • LIPO(Sec/SPII): 0.119325
  • predicted transmembrane helices (TMHMM): 4

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTLFFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKNLVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]