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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS03305 [old locus tag: SA0572 ]
  • pan locus tag?: SAUPAN002483000
  • symbol: SA_RS03305
  • pan gene symbol?:
  • synonym:
  • product: alpha/beta hydrolase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS03305 [old locus tag: SA0572 ]
  • symbol: SA_RS03305
  • product: alpha/beta hydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 665307..666095
  • length: 789
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (665307..666095) NCBI
  • BioCyc: SA_RS03305 BioCyc
  • MicrobesOnline: see SA0572

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAATAAAGTCACAATTAATCCTCAAATCCAATTAACTTATCAAATTGAAGGTAAAGGG
    GATCCTATAATATTACTTCATGGATTGGATGGTAATTTAGCTGGATTTGAAGATTTGCAA
    CATCAACTAGCATCATCATATAAAGTACTTACTTACGATTTAAGAGGTCATGGCAAGTCT
    TCTAAAAGTGAATCATACGATTTAAACGATCACGTTGAGGATTTAAAAATTCTAATGGAG
    AAGTTAAATATTCATGAGGCACATATTCTAGGACATGATTTAGGTGGGGTAGTTGCTAAG
    TTATTTACAGATAAATATGCTTATCGTGTAAAATCATTAACTACCATTGCATCGAAGAAA
    GATGACTTAATACACAGCTTTACTCAATTGTTAATACAATATCAAGATGATATAGCGGGT
    TTTAATAAGTCTGAAGCGTATATTCTTTTATTTTCTAAATTGTTTAGAAATCAAGAGAAG
    ACGATGAAATGGTATCAAAAACAAAGAATATATAGCATTAAGTCTGAGGATGATAGTGCG
    GTGGCAATTCGTTCATTAATTTTGCATAAAGATGAACCTATGTATTTAAAAAAACGTACA
    TGTGTACCTACTTTGTTAATTAATGGGGAACATGATCCTTTGATTAAAGATAAGAATCAT
    TTTAAATTGGAAGCGCATTTTTTAAATGTTACGAAAAAAATCTTCGAACATTCAGGACAT
    GCACCGCATATTGAAGAACCAGAAGCATTTATGAATTATTATTTAAATTTTTTAAAAAGC
    GTATCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    789

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS03305 [old locus tag: SA0572 ]
  • symbol: SA_RS03305
  • description: alpha/beta hydrolase
  • length: 262
  • theoretical pI: 7.55088
  • theoretical MW: 30201.4
  • GRAVY: -0.412214

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Energy metabolism Other 3-oxoadipate enol-lactonase (TIGR02427; EC 3.1.1.24; HMM-score: 78.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 76.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin pimelyl-[acyl-carrier protein] methyl ester esterase (TIGR01738; EC 3.1.1.85; HMM-score: 73)
    pyrimidine utilization protein D (TIGR03611; HMM-score: 68.6)
    and 6 more
    proline-specific peptidase (TIGR01250; HMM-score: 59.9)
    Metabolism Energy metabolism Photosynthesis putative magnesium chelatase accessory protein (TIGR03056; HMM-score: 48)
    prolyl aminopeptidase (TIGR01249; EC 3.4.11.5; HMM-score: 35.3)
    2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (TIGR03343; EC 3.7.1.-; HMM-score: 34.6)
    Metabolism Energy metabolism Other poly(3-hydroxyalkanoate) depolymerase (TIGR02240; EC 3.1.1.-; HMM-score: 25.1)
    Metabolism Amino acid biosynthesis Aspartate family homoserine O-acetyltransferase (TIGR01392; EC 2.3.1.31; HMM-score: 13.2)
  • TheSEED: see SA0572
  • PFAM:
    AB_hydrolase (CL0028) Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 100.5)
    and 10 more
    Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 61.2)
    Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 54.5)
    PGAP1; PGAP1-like protein (PF07819; HMM-score: 20.8)
    FSH1; Serine hydrolase (FSH1) (PF03959; HMM-score: 20.6)
    Ndr; Ndr family (PF03096; HMM-score: 18.6)
    DUF915; Alpha/beta hydrolase of unknown function (DUF915) (PF06028; HMM-score: 16.6)
    Abhydrolase_5; Alpha/beta hydrolase family (PF12695; HMM-score: 16.4)
    Abhydrolase_4; TAP-like protein (PF08386; HMM-score: 13.9)
    Thioesterase; Thioesterase domain (PF00975; HMM-score: 12.4)
    Lipase_3; Lipase (class 3) (PF01764; HMM-score: 12.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007481
    • TAT(Tat/SPI): 0.000325
    • LIPO(Sec/SPII): 0.000767
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLASSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLFTDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEKTMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDKNHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFMNYYLNFLKSVS

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]