PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS03350 [old locus tag: SA0581 ]
• pan locus tag^?: SAUPAN002497000
• symbol: SA_RS03350
• pan gene symbol^?: mnhD2
• synonym:
• product: cation:proton antiporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS03350 [old locus tag: SA0581 ]
• symbol: SA_RS03350
• product: cation:proton antiporter
• replicon: chromosome
• strand: +
• coordinates: 673120..674616
• length: 1497
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (673120..674616) NCBI
• BioCyc: SA_RS03350 BioCyc
• MicrobesOnline: see SA0581

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  
  ATGCTAAGTAACTTATTGATTTTACCAATGTTATTACCATTCCTTTGTGCCTTAATCCTT
  GTATTTTTAAAAAATAATGATCGTATTTCTAAATATTTATACTTAGGTACAATGACTATC
  ACCACAATTATTTCATTAATGCTATTAATTTATGTTCAGCGTCACCGTCCAATTACGCTA
  GACTTTGGAGGATGGTCAGCGCCCTTTGGTATACAGTTTTTAGGAGATTCTTTAAGTTTG
  ATTATGGTTACAACCGCTTCGTTTGTGATTACTTTAATTATGGCATACGGATTTGGGCGT
  GGCGAACATAAAGCAAATCGTTATCACTTGCCATCGTTCATATTATTTTTAAGTGTTGGC
  GTGATAGGCTCTTTTCTAACATCAGATTTATTTAATTTATACGTCATGTTTGAAATTATG
  TTACTAGCGTCATTTGTACTCATTACACTTGGACAATCTGTAGAACAATTACGTGCTGCA
  ATTATTTATGTTGTCTTGAATATTATTGGTTCATGGCTATTCTTATTAGGTATAGGTTTA
  CTTTATAAAACAGTAGGTACATTAAACTTTTCACATATTGCAATGCGTTTGAATGACATG
  GGAGATAATCGCACTGTTACAATGATTTCATTAATCTTCTTAGTCGCATTTAGTGCGAAA
  GCAGCGCTGGTCCTTTTTATGTGGCTACCCAAAGCCTACGCTGTGTTAAATACTGAGCTT
  GCAGCATTATTTGCAGCGTTAATGACCAAAGTAGGGGCCTATGCATTAATTCGATTCTTC
  ACTTTACTATTTGATCAACATAATGATCTCATACATCCATTGCTAGCAACTATGGCTGCT
  ATAACTATGGTCATCGGCGCTATAGGTGTCATTGCTTATAAAGATATTAAAAAGATTGCA
  GCTTACCAAGTCATAATCTCAATAGGATTTATCATTTTAGGTTTAGGAACAAACACGTTT
  GCAGGTATTAATGGTGCAATATTTTATTTGGTAAATGACATTGTTGTAAAAACATTGCTA
  TTCTTTATTATTGGTAGTTTAGTTTACATTACAGGCTATCGACAATATCAATATTTGAAT
  GGCTTAGCTAAAAAAGAACCTTTATTTGGAGTTGCGTTTATTATAATGATTTTTGCTATT
  GGCGGCGTGCCTCCATTTAGTGGCTTTCCGGGGAAAGTACTTATTTTCCAAGGTGCATTG
  CAAAATGGCAATTATATTGGACTAGCGTTAATGATTATTACTAGTCTAATTGCAATGTAC
  AGTTTATTTAGGATATTTTTTTATATGTATTTTGGAGATAAAGATGGGGAGGAAGTTAAT
  TTTAAGAAAATCCCGCTATATCGAAAAAGAATTTTAAGTATTTTAGTAGTTGTGGTTATC
  GCAATCGGAATTGCTGCACCTGTTGTGTTAAATGTTACAAGTGATGCAACTGAGTTGAAC
  ACGAGTGATCAATTATATCAAAAACTTGTAAATCCGCATTTGAAAGGAGAGGACTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1497
  

⊟Protein

⊟General

• locus tag: SA_RS03350 [old locus tag: SA0581 ]
• symbol: SA_RS03350
• description: cation:proton antiporter
• length: 498
• theoretical pI: 9.6341
• theoretical MW: 55186.9
• GRAVY: 0.920482

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds multicomponent K+:H+antiporter (TIGR00944; HMM-score: 366.7)
  and 5 more
  Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain M (TIGR01972; HMM-score: 203.2)
  Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain N (TIGR01770; HMM-score: 175.1)
  Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain L (TIGR01974; EC 1.6.-.-; HMM-score: 130.4)
  Metabolism Transport and binding proteins Cations and iron carrying compounds monovalent cation:proton antiporter (TIGR00940; HMM-score: 93.8)
  NAD(P)H dehydrogenase, subunit NdhF3 family (TIGR01960; HMM-score: 67.7)
• TheSEED: see SA0581
• PFAM:
  ComplexI-N (CL0425) Proton_antipo_M; Proton-conducting membrane transporter (PF00361; HMM-score: 189.6)
  and 3 more
  no clan defined Proton_antipo_N; NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus (PF00662; HMM-score: 17.5)
  DUF1490; Protein of unknown function (DUF1490) (PF07371; HMM-score: 14)
  DUF2749; Protein of unknown function (DUF2749) (PF10907; HMM-score: 10.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 14
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00738
  • TAT(Tat/SPI): 0.000676
  • LIPO(Sec/SPII): 0.039209
• predicted transmembrane helices (TMHMM): 14

⊟Accession numbers

• GI: 446873290 NCBI
• RefSeq: WP_000950546 NCBI
• UniProt: see SA0581

⊟Protein sequence

• MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTIISLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGEHKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAAIIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFSAKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLATMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDIVVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGKVLIFQGALQNGNYIGLALMIITSLIAMYSLFRIFFYMYFGDKDGEEVNFKKIPLYRKRILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications