PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS04125 [old locus tag: SA0724 ]
• pan locus tag^?: SAUPAN002697000
• symbol: SA_RS04125
• pan gene symbol^?: —
• synonym:
• product: epimerase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS04125 [old locus tag: SA0724 ]
• symbol: SA_RS04125
• product: epimerase
• replicon: chromosome
• strand: -
• coordinates: 828460..829362
• length: 903
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (828460..829362) NCBI
• BioCyc: SA_RS04125 BioCyc
• MicrobesOnline: see SA0724

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
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  481
  541
  601
  661
  721
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  841
  901
  
  ATGAAACAATACTTAATTACTGGTGGGACTGGTATGGTTGGATCTCAATTAGTTAATGAA
  ATTAAAAAATCAGATTCACATATCACGATATTAACGCGACACGACCAAATTTCAAATGAT
  AAGAAAATTTCATATGTCAACTGGGCTAAATCTGGGTGGGAACACAAAGTTCCTCAAAAT
  ATCGATGTGGTCATCAACTTAGCAGGTGCTACACTGAATAAACGATGGACACCGGAATAT
  AAACAAACGCTAATGCTAAGTAGAATTCAATCTACGCAAGCTTTATATGAATTGTTTAAA
  TCACGTAATAAAGCACCTAAAGTTTTATTTAATGCTAGCGCCACTGGTTACTATCCTCCT
  GATTTATTTATGAGTTACACAGAAGTTTATAAAACTTTACCATTTGATTTCTTATCAGAT
  ATTGTGTATCAATGGGAACGTTTCGCACAACAATTTGAACAATTAGGTACGCGAGTTGTC
  ATCGGTCGTTTCGGTATGATTTTATCGAATGAAGGCGGTGCGTTACAAACAATGAAACTA
  CCATACAAATATTACATTGGTGGTAAATTAGGTTCTGGTCAACAATGGTATTCATGGATT
  CATATCAATGATTTAATTCAAGCTATTTTATTTTTAATAAATAACGAGTCAGCTAGTGGT
  CCGTTTAATTTAACTGCACCTATACCTGAGCGTCAAAATTTATTTGGCTACACTTTAGCA
  AGAGCTATGCATAAGCCCCATGAAACTTGGGCACCAAGTCTTGCAATGCGTCTCATACTT
  GGTCAAATGTCAACAGTAGTATTGGATACTCAAAAAGTATTACCTAATAAAATTCAAGCA
  TTGGGATTCCAATTTAAATATAGTAATTTAAAAATGGCACTTGAAGATTTAATCAAAGAA
  TAA
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  903
  

⊟Protein

⊟General

• locus tag: SA_RS04125 [old locus tag: SA0724 ]
• symbol: SA_RS04125
• description: epimerase
• length: 300
• theoretical pI: 9.88206
• theoretical MW: 34272.4
• GRAVY: -0.229

⊟Function

• TIGRFAM:
  Hypothetical proteins Conserved TIGR01777 family protein (TIGR01777; HMM-score: 363.1)
  and 5 more
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides ADP-glyceromanno-heptose 6-epimerase (TIGR02197; EC 5.1.3.20; HMM-score: 31)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides dTDP-glucose 4,6-dehydratase (TIGR01181; EC 4.2.1.46; HMM-score: 20.3)
  hopanoid-associated sugar epimerase (TIGR03466; HMM-score: 15.6)
  Metabolism Energy metabolism Sugars UDP-glucose 4-epimerase GalE (TIGR01179; EC 5.1.3.2; HMM-score: 14.5)
  thioester reductase domain (TIGR01746; HMM-score: 14.5)
• TheSEED: see SA0724
• PFAM:
  NADP_Rossmann (CL0063) Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 68.4)
  no clan defined DUF1731; Domain of unknown function (DUF1731) (PF08338; HMM-score: 62)
  and 9 more
  NADP_Rossmann (CL0063) NAD_binding_4; Male sterility protein (PF07993; HMM-score: 25.2)
  GDP_Man_Dehyd; GDP-mannose 4,6 dehydratase (PF16363; HMM-score: 20.8)
  KR; KR domain (PF08659; HMM-score: 18.5)
  RmlD_sub_bind; RmlD substrate binding domain (PF04321; HMM-score: 15.5)
  Polysacc_synt_2; Polysaccharide biosynthesis protein (PF02719; HMM-score: 14.7)
  Semialdhyde_dh; Semialdehyde dehydrogenase, NAD binding domain (PF01118; HMM-score: 14.4)
  NmrA; NmrA-like family (PF05368; HMM-score: 12.9)
  NAD_binding_10; NAD(P)H-binding (PF13460; HMM-score: 11.7)
  adh_short; short chain dehydrogenase (PF00106; HMM-score: 11.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.029732
  • TAT(Tat/SPI): 0.000589
  • LIPO(Sec/SPII): 0.005143
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446739048 NCBI
• RefSeq: WP_000816304 NCBI
• UniProt: see SA0724

⊟Protein sequence

• MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKISYVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSRNKAPKVLFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVVIGRFGMILSNEGGALQTMKLPYKYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESASGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPNKIQALGFQFKYSNLKMALEDLIKE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications