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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS04440 [old locus tag: SA0778 ]
  • pan locus tag?: SAUPAN002899000
  • symbol: SA_RS04440
  • pan gene symbol?: sufB
  • synonym:
  • product: Fe-S cluster assembly protein SufB

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS04440 [old locus tag: SA0778 ]
  • symbol: SA_RS04440
  • product: Fe-S cluster assembly protein SufB
  • replicon: chromosome
  • strand: +
  • coordinates: 884238..885635
  • length: 1398
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (884238..885635) NCBI
  • BioCyc: SA_RS04440 BioCyc
  • MicrobesOnline: see SA0778

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCTAAAAAAGCACCTGATGTTGGGGATTATAAATATGGATTCCACGACGATGATGTA
    TCCATTTTCAGATCAGAACGTGGTTTAACTGAGAATATCGTTAGAGAAATTTCTAACATG
    AAAAATGAGCCGGAATGGATGTTAGATTTCCGTCTTAAATCATTAAAATTGTTTTATAAA
    ATGCCAATGCCTCAATGGGGTGGCGACTTATCAGAATTGAATTTCGATGACATTACTTAC
    TATGTAAAGCCTTCAGAACAAGCTGAACGTTCATGGGATGAAGTGCCAGAAGAAATTAAA
    AGAACTTTCGATAAATTAGGAATTCCTGAAGCTGAACAAAAATATTTAGCTGGTGTTTCT
    GCTCAATATGAATCTGAAGTTGTTTACCATAATATGGAAAAAGAACTTGAAGAAAAAGGT
    ATTATCTTTAAAGATACAGATAGTGCTTTACAAGAAAATGAAGAATTATTCAAAAAATAC
    TTTGCTTCTGTAGTACCTGCAGCAGATAACAAATTTGCGGCGTTAAACTCAGCAGTATGG
    TCAGGTGGTTCGTTCATTTATGTACCTAAAAATATCAAACTAGATACGCCACTACAAGCT
    TATTTCCGTATTAACTCTGAGAACATGGGTCAATTTGAACGTACATTAATCATTGCTGAT
    GAAGGTGCTTCTGTACATTACGTAGAAGGTTGTACTGCACCAGTTTATACAACTAGTTCT
    TTACACTCTGCTGTTGTGGAAATCATTGTGCATAAAGATGCGCACGTTCGTTATACTACG
    ATTCAAAACTGGGCGAACAATGTATACAATTTAGTTACAAAACGTACTTTTGTTTATGAA
    AACGGAAATATGGAATGGGTAGATGGTAACTTAGGTTCTAAGTTAACGATGAAATATCCA
    AACTGTGTTCTTTTAGGTGAAGGTGCAAAAGGTAGTACATTATCTATTGCATTTGCTGGT
    AAAGGACAAGTTCAAGATGCCGGTGCTAAAATGATTCATAAAGCGCCAAATACATCTTCA
    ACGATTGTTTCTAAATCTATTTCTAAAAATGGTGGTAAAGTTATTTATCGCGGTATTGTT
    CATTTTGGACGTAAAGCAAAAGGTGCTCGTTCAAATATTGAATGTGATACATTAATCTTA
    GATAACGAATCAACATCAGATACAATTCCATATAACGAAGTATTCAACGATCAAATATCA
    TTAGAACATGAAGCCAAGGTTTCAAAAGTTTCTGAAGAACAATTATTTTATCTAATGAGT
    CGTGGTATTTCTGAAGAAGAAGCGACAGAAATGATTGTTATGGGATTCATCGAACCATTT
    ACAAAAGAACTTCCAATGGAATACGCGGTCGAAATGAACCGTTTAATCAAGTTCGAAATG
    GAAGGTAGTATTGGATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1398

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS04440 [old locus tag: SA0778 ]
  • symbol: SA_RS04440
  • description: Fe-S cluster assembly protein SufB
  • length: 465
  • theoretical pI: 4.81321
  • theoretical MW: 52531.1
  • GRAVY: -0.397849

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other FeS assembly protein SufB (TIGR01980; HMM-score: 767.9)
    and 1 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other FeS assembly protein SufD (TIGR01981; HMM-score: 201.1)
  • TheSEED: see SA0778
  • PFAM:
    no clan defined UPF0051; Uncharacterized protein family (UPF0051) (PF01458; HMM-score: 250.9)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003263
    • TAT(Tat/SPI): 0.000265
    • LIPO(Sec/SPII): 0.000357
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEPEWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRTFDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKYFASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLIIADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRTFVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHKAPNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYNEVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAVEMNRLIKFEMEGSIG

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]