From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS04675 [old locus tag: SA0822 ]
  • pan locus tag?: SAUPAN003068000
  • symbol: SA_RS04675
  • pan gene symbol?: argG
  • synonym:
  • product: argininosuccinate synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS04675 [old locus tag: SA0822 ]
  • symbol: SA_RS04675
  • product: argininosuccinate synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 927310..928515
  • length: 1206
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (927310..928515) NCBI
  • BioCyc: SA_RS04675 BioCyc
  • MicrobesOnline: see SA0822

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAAAGAGAAAATTGTTTTAGCATATTCAGGAGGACTAGATACAAGTGTGGCCGTTCAA
    TGGCTTATCGACAAAGGATACGACGTTGTAGCTTGTTGCCTAGATGTTGGTGAAGGTAAA
    GATTTAGACATCGTTTATAAAAAAGCTTTAGATATGGGAGCAGTTGAATGTCATATTATT
    GATGCAACAAAAGAATTTAGTGATGAGTATGTAAGTTATGCAATCAAAGGAAATTTAATG
    TATGAAAATGCATATCCATTAGTTTCAGCTTTATCAAGACCATTAATCGCTAAAAAATTA
    GTAGAGATTGCTGAGAAAACAAATTCAGTAGGTATTGCACATGGTTGTACTGGTAAAGGG
    AATGACCAAGTACGTTTCGAAGTTGCCATTAAAGCATTAAACCCATCATTGAAAGCATTC
    GCACCTGTACGTGAGTGGGCATGGAGTCGTGAAGAAGAAATCGATTATGCAATTAAACAT
    AATATCCCTGTATCAATCAACCATGATTCACCTTATTCTATCGATCAAAATCTATGGGGC
    AGAGCGAATGAATGTGGTATTTTAGAAGATCCTTATGCTGCGCCACCAGAGGATGCGTTC
    GATCTAACAAATGCTTTAGAAGAAACACCAGATACTGCTGATGAAATCATTTTAACGTTT
    GATAAAGGCATCCCAGTTCAAATTGATGGCAAAACATATGAATTAGACGATTTAATTTTA
    ACGTTGAATGCATTAGCTGGTAAGCATGGTATCGGAAGAATTGACCATGTAGAAAATAGA
    CTTGTAGGTATCAAATCAAGAGAAATTTATGAGGCACCTGCTGCAGAAGTTATTTTAAAA
    GCGCATAAAGCATTAGAAACGATTACGTTAACTAAAGATGTCGCACACTTTAAACCAATC
    ATTGAGAAGCAATTTGCTGAACAACTATACAATGGACTTTGGTTCTCACCTTTAACTGAT
    AGCTTGAAATTATTTATTGATAGTACTCAGCAATACGTAAGTGGTGATGTCAGAATTAAA
    TTATTCAAAGGTAATGCCATCGTGAATGGTAGAAAATCACCTTACACATTATATGATGAA
    AAATTAGCAACTTATACAAAAGAAGATGCATTTAATCAAGACGCTGCTGTTGGCTTTATC
    GATATCTATGGTTTACCTACTCAAGTAAATGCGATGTTGCATGGAGGCTATAGCAATGAG
    CAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1206

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS04675 [old locus tag: SA0822 ]
  • symbol: SA_RS04675
  • description: argininosuccinate synthase
  • length: 401
  • theoretical pI: 4.61504
  • theoretical MW: 44455.1
  • GRAVY: -0.182544

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.4.5?  ExPASy
    Argininosuccinate synthase ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Glutamate family argininosuccinate synthase (TIGR00032; EC 6.3.4.5; HMM-score: 524.1)
    and 5 more
    Unknown function General queuosine biosynthesis protein QueC (TIGR00364; HMM-score: 14.3)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (TIGR00420; EC 2.1.1.61; HMM-score: 13.1)
    Hypothetical proteins Conserved TIGR00268 family protein (TIGR00268; HMM-score: 12.7)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 12.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 12.5)
  • TheSEED: see SA0822
  • PFAM:
    HUP (CL0039) Arginosuc_synth; Arginosuccinate synthase (PF00764; HMM-score: 556.4)
    and 3 more
    tRNA_Me_trans; tRNA methyl transferase (PF03054; HMM-score: 16.9)
    QueC; Queuosine biosynthesis protein QueC (PF06508; HMM-score: 14.2)
    ThiI; Thiamine biosynthesis protein (ThiI) (PF02568; HMM-score: 11.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.055477
    • TAT(Tat/SPI): 0.000643
    • LIPO(Sec/SPII): 0.011175
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDIVYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVEIAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKHNIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIILTFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAEVILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVSGDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAMLHGGYSNEQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:
    SA_RS02960elongation factor Tu  [1] (data from MRSA252)
    SA_RS08505aldehyde dehydrogenase  [1] (data from MRSA252)
    SA_RS1168030S ribosomal protein S5  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: ArgR*, CodY see SA0822

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SA_RS04675&oldid=80814"