PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS05610 [old locus tag: SA0990 ]
• pan locus tag^?: SAUPAN003381000
• symbol: SA_RS05610
• pan gene symbol^?: polX
• synonym:
• product: DNA polymerase/3'-5' exonuclease PolX

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS05610 [old locus tag: SA0990 ]
• symbol: SA_RS05610
• product: DNA polymerase/3'-5' exonuclease PolX
• replicon: chromosome
• strand: +
• coordinates: 1120374..1122086
• length: 1713
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (1120374..1122086) NCBI
• BioCyc: SA_RS05610 BioCyc
• MicrobesOnline: see SA0990

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACAAAAAAAGATGTTATCAAACTATTAGAACAAATTGCTACTTATATGGAATTAAAA
  GGGGAAAATACTTTTAAAATATCAGCGTATCGAAAAGCAGCTCAAAGTCTTGAATTAGAT
  GAACGACCATTAGATGAAATATCTGATGTAACGGAGTTAAAAGGCATTGGTAAAGGTGTT
  GCAGAAGTAATCAATGATTACCGTGAGACCGGTGAATCTCAGTATTTACAGCAATTACAG
  GAAGAAGTTCCGGAAGGTCTTATTCCACTTTTGAAAATTCAAGGACTTGGAAGCAAGAAA
  ATTGCTAAGCTATATAAAGAGTTGAATATTGTTGATAAAGCGTCACTTCAAGTTGCTTGT
  GAAAATGGAAAAGTTAGTGAATTAAGCGGATTTGCTAAGAAAACGGAACAAAACATATTA
  GAAGCTGTGAAACAACTTGGTGCTAAGAAAGATAGATATCCAATTGATCAAATGAGAAGA
  CTTAATCAAGAAATCATTGATTATATAGATACATTAAATTATATCGATCAATATTCATCT
  GCAGGAAGCTTCCGTCGTTTTAAAGAAATGAGCAAAGATTTAGATTTCATAATAAGTACC
  GATAACCCAAAAGCAGTGCAGCAGCAATTATTAAATATTCCCAATAAAGTAAAAGAAGTT
  GCAGTGGGGAACACAAAAGTTTCATTAGAATTAGCGTATGATGATGAAACGATTGGTGTC
  GATTTTCGATTAATTGAACCAAGGGCTTTTTATCATACATTGCAACATTTTACTGGGTCA
  AAAGAACATAATATAAGAATTCGACAACTTGCTAAAGCACGTGATGAAAAAGTAAGTGAA
  TATGGAATTGAACAAGCTGATGGTACATTAATTCAATATGATAGTGAAGCCAAGATATAT
  GAACATTTTAATGTGAATTTTATACCACCTGCTATGCGAGAAGATGGTAGCGAATTTGAT
  AAAGATCTAAGTAATATCATTACATTAGATGATATTAATGGTGATATTCATATGCATACA
  ACGTATAGTGATGGTGCGTTTTCTATTCGAGACATGGTAGAAGCAAATATCGCAAAAGGT
  TATAAATTCATGGTAATTACTGATCATTCACAAAGTTTACGTGTTGCTAATGGCTTACAA
  GTGGAAAGACTTTTAAGACAAAACGAAGAAATTAAGGCTTTAGATAAAGAATATAGTGAA
  ATTGATATTTATTCAGGTACAGAAATGGATATATTACCTGATGGCTCGCTGGATTATGAT
  GATGAAATTTTAGCACAACTTGATTATGTAATTGGAGCTATTCATCAAAGCTTTAACCAA
  TCAGAAGAACAAATTATGGAACGATTAGCTAATGCATGTCGCAATCCATACGTGCGACAT
  ATAGCGCATCCAACAGGGCGTATTATAGGTAGAAGAGATGGTTATAAACCGAATATTGAA
  CAATTAATGGCATTAGCTGAAGAAACGAATACAGTATTAGAAATTAATGCCAATCCACAT
  CGACTGGATCTGAACGCTGAAATCGTTCGTAAATATCCAAATGTGAAATTAACTATTAAC
  ACTGATGCGCATCATACAAATCATTTAGATTTTATGAATTATGGCGTAGCAACTGCGCAA
  AAAGGATTTGTAACAAAAGATAGAGTGATTAACGCATTATCGCGTGAAGCTTTTAAAGAC
  TTTATTGAAAATAATATAAAACTTAAGAAATAG
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⊟Protein

⊟General

• locus tag: SA_RS05610 [old locus tag: SA0990 ]
• symbol: SA_RS05610
• description: DNA polymerase/3'-5' exonuclease PolX
• length: 570
• theoretical pI: 5.07539
• theoretical MW: 64992.1
• GRAVY: -0.521403

⊟Function

• TIGRFAM:
  histidinol phosphate phosphatase, HisJ family (TIGR01856; HMM-score: 37.9)
  and 2 more
  Cellular processes Cellular processes DNA transformation comEA protein (TIGR01259; HMM-score: 11.5)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvA (TIGR00084; EC 3.6.4.12; HMM-score: 9.6)
• TheSEED: see SA0990
• PFAM:
  HHH (CL0198) HHH_8; Helix-hairpin-helix domain (PF14716; HMM-score: 72.7)
  no clan defined DNA_pol_B_thumb; DNA polymerase beta thumb (PF14791; HMM-score: 58.9)
  and 4 more
  Amidohydrolase (CL0034) PHP; PHP domain (PF02811; HMM-score: 33.1)
  HHH (CL0198) HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 25)
  NTP_transf (CL0260) DNA_pol_B_palm; DNA polymerase beta palm (PF14792; HMM-score: 21.2)
  HHH (CL0198) HHH_3; Helix-hairpin-helix motif (PF12836; HMM-score: 15.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006631
  • TAT(Tat/SPI): 0.000422
  • LIPO(Sec/SPII): 0.000559
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446084083 NCBI
• RefSeq: WP_000161938 NCBI
• UniProt: see SA0990

⊟Protein sequence

• MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPLDEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIAKLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRRLNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVKEVAVGNTKVSLELAYDDETIGVDFRLIEPRAFYHTLQHFTGSKEHNIRIRQLAKARDEKVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITLDDINGDIHMHTTYSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIKALDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLANACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLNAEIVRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNIKLKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications