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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA_RS07425 [old locus tag: SA1310 ]
- pan locus tag?: SAUPAN003943000
- symbol: SA_RS07425
- pan gene symbol?: ansA
- synonym:
- product: L-asparaginase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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961ATGAAACATCTACTTGTTATTCATACTGGTGGCACCATTAGTATGTCACAAGACCAATCT
AATAAAGTAGTAACAAATGATATCAACCCTATTTCAATGCATCAAGATGTCATAAATCAA
TATGCACAAATAGATGAATTAAATCCTTTTAATGTACCATCACCTCATATGACAATCCAA
CATGTTAAACAATTAAAGGATATTATTTTAGAAGCAGTAACAAATAAATATTATGATGGT
TTCGTTATCACGCATGGTACCGATACGTTAGAAGAAACTGCCTTTTTACTTGATTTAATA
TTAGGTATCGAGCAACCTGTTGTTATTACTGGCGCAATGCGCTCGTCTAATGAAATTGGT
TCTGACGGATTATATAATTATATTTCCGCTATTCGCGTTGCCTCTGATGAAAAGGCCCGT
CATAAAGGCGTGATGGTTGTATTTAATGATGAAATTCATACGGCGCGTAATGTTACCAAA
ACACATACGTCTAATACAAACACATTTCAAAGTCCAAATCATGGTCCGCTAGGTGTATTG
ACAAAGGATCGTGTGCAATTCCATCATATGCCATATCGCCAACAAGCATTGGAAAATGTC
AATGACAAACTAAATGTACCATTAGTAAAAGCATATATGGGTATGCCAGGTGACATTTTT
AGTTTTTATAGTCGTGAAGGTATCGATGGTATGGTTATTGAAGCGTTAGGACAAGGCAAC
ATACCTCCAAGCGCATTAGAAGGCATTCAACAATTAGTATCTTTAAATATACCTATTGTG
CTAGTTTCACGTTCCTTTAATGGTATTGTGAGTCCAACTTACGCATACGATGGTGGTGGT
TACCAACTCGCACAACAAGGTTTTATTTTTTCTAACGGTTCGAATGGTCCAAAAGCAAGA
TTAAAATTATTAGTTGCGTTAAGCAACAATTTAGATAAAGCTGAAATCAAATCATATTTT
GAATTATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA_RS07425 [old locus tag: SA1310 ]
- symbol: SA_RS07425
- description: L-asparaginase
- length: 322
- theoretical pI: 6.3723
- theoretical MW: 35552.2
- GRAVY: -0.165217
⊟Function[edit | edit source]
- TIGRFAM: Energy metabolism Amino acids and amines L-asparaginase, type II (TIGR00520; EC 3.5.1.1; HMM-score: 230.6)and 2 moreL-asparaginase, type I (TIGR00519; EC 3.5.1.1; HMM-score: 174.6)Protein synthesis tRNA aminoacylation glutamyl-tRNA(Gln) amidotransferase, subunit D (TIGR02153; EC 6.3.5.-; HMM-score: 130.9)
- TheSEED: see SA1310
- PFAM: no clan defined Asparaginase; Asparaginase, N-terminal (PF00710; HMM-score: 201)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.67
- Cytoplasmic Membrane Score: 0.01
- Cellwall Score: 0.15
- Extracellular Score: 0.17
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.037167
- TAT(Tat/SPI): 0.000591
- LIPO(Sec/SPII): 0.02003
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQIDELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILGIEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTKTHTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGDIFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAYDGGGYQLAQQGFIFSNGSNGPKARLKLLVALSNNLDKAEIKSYFEL
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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