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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS08215 [old locus tag: SA1457 ]
  • pan locus tag?: SAUPAN004236000
  • symbol: SA_RS08215
  • pan gene symbol?: hisS
  • synonym:
  • product: histidine--tRNA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS08215 [old locus tag: SA1457 ]
  • symbol: SA_RS08215
  • product: histidine--tRNA ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1662578..1663840
  • length: 1263
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1662578..1663840) NCBI
  • BioCyc: SA_RS08215 BioCyc
  • MicrobesOnline: see SA1457

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGATTAAAATACCTAGAGGGACGCAGGATATTTTACCTGAAGATTCAAAGAAATGGCGT
    TACATTGAAAATCAATTAGATGAATTAATGACATTTTATAATTATAAAGAAATAAGAACA
    CCAATTTTTGAAAGTACAGATCTTTTTGCAAGAGGTGTTGGTGATTCAACCGATGTCGTA
    CAAAAAGAAATGTATACATTTAAAGATAAAGGCGATAGAAGTATTACATTAAGACCTGAG
    GGAACAGCTGCAGTTGTGCGTTCATATATTGAACATAAAATGCAAGGTAATCCAAACCAA
    CCAATTAAACTTTATTACAATGGACCGATGTTTAGATATGAACGTAAGCAAAAAGGGCGC
    TATCGTCAATTTAATCAATTTGGTGTAGAAGCTATTGGTGCTGAAAATCCTAGCGTAGAT
    GCAGAAGTATTAGCTATGGTTATGCATATTTATCAATCATTTGGATTAAAACATTTAAAG
    CTTGTTATTAATAGTGTAGGGGATATGGCGTCTCGAAAAGAATATAACGAAGCGTTAGTG
    AAACACTTTGAACCAGTAATTCATGAATTTTGTTCAGATTGTCAATCACGTTTGCATACA
    AATCCGATGCGAATTTTGGATTGTAAAGTTGACCGTGATAAAGAAGCGATTAAGACTGCA
    CCTAGAATCACTGATTTCTTAAATGAGGAATCTAAGGCATATTATGAACAAGTAAAAGCT
    TATTTAGATGATTTAGGTATTCCATATATTGAAGATCCTAACTTAGTTCGTGGATTGGAT
    TATTATACACATACAGCATTTGAATTAATGATGGATAATCCTAACTATGATGGTGCCATT
    ACAACGCTTTGTGGTGGTGGCCGTTATAATGGTTTATTAGAATTGCTAGATGGTCCAAGT
    GAAACAGGTATTGGTTTTGCGCTAAGTATAGAACGATTATTGCTTGCACTTGAAGAAGAA
    GGTATCGAATTAGATATTGAAGAAAACTTAGATTTATTCATTGTTACAATGGGTGATCAA
    GCAGATCGATATGCTGTGAAGTTATTAAATCATTTGAGACATAATGGTATTAAAGCAGAT
    AAAGACTACTTACAGCGTAAAATTAAAGGACAAATGAAACAAGCAGACCGTTTAGGTGCC
    AAGTTTACAATCGTTATTGGTGATCAAGAATTAGAAAATAATAAAATCGATGTTAAAAAT
    ATGACAACTGGTGAATCTGAAACAATTGAATTAGACGCATTAGTCGAATATTTTAAGAAG
    TAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1263

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS08215 [old locus tag: SA1457 ]
  • symbol: SA_RS08215
  • description: histidine--tRNA ligase
  • length: 420
  • theoretical pI: 5.01807
  • theoretical MW: 48282.6
  • GRAVY: -0.519048

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.21?  ExPASy
    Histidine--tRNA ligase ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation histidine--tRNA ligase (TIGR00442; EC 6.1.1.21; HMM-score: 510.5)
    and 7 more
    Metabolism Amino acid biosynthesis Histidine family ATP phosphoribosyltransferase, regulatory subunit (TIGR00443; HMM-score: 169.6)
    Genetic information processing Protein synthesis tRNA aminoacylation threonine--tRNA ligase (TIGR00418; EC 6.1.1.3; HMM-score: 57.1)
    Genetic information processing Protein synthesis tRNA aminoacylation proline--tRNA ligase (TIGR00409; EC 6.1.1.15; HMM-score: 31.9)
    Genetic information processing Protein synthesis tRNA aminoacylation proline--tRNA ligase (TIGR00408; EC 6.1.1.15; HMM-score: 24.6)
    Genetic information processing Protein synthesis tRNA aminoacylation pyrrolysine--tRNA ligase, C-terminal region (TIGR02367; EC 6.1.1.26; HMM-score: 19.6)
    Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase, alpha subunit (TIGR00468; EC 6.1.1.20; HMM-score: 14)
    Genetic information processing Protein synthesis tRNA aminoacylation glycine--tRNA ligase (TIGR00389; EC 6.1.1.14; HMM-score: 12.6)
  • TheSEED: see SA1457
  • PFAM:
    tRNA_synt_II (CL0040) tRNA-synt_His; Histidyl-tRNA synthetase (PF13393; HMM-score: 162.3)
    and 4 more
    tRNA-synt_2b; tRNA synthetase class II core domain (G, H, P, S and T) (PF00587; HMM-score: 72.7)
    IIaaRS-ABD (CL0458) HGTP_anticodon; Anticodon binding domain (PF03129; HMM-score: 70.2)
    tRNA_synt_II (CL0040) tRNA-synt_2d; tRNA synthetases class II core domain (F) (PF01409; HMM-score: 15.8)
    IIaaRS-ABD (CL0458) HGTP_anticodon2; Anticodon binding domain of tRNAs (PF12745; HMM-score: 15.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001648
    • TAT(Tat/SPI): 0.000122
    • LIPO(Sec/SPII): 0.000183
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFESTDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPIKLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLKLVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIKTAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNYDGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFIVTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELENNKIDVKNMTTGESETIELDALVEYFKK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: T-box(Asn) see SA1457

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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