PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS10085 [old locus tag: SA1754 ]
• pan locus tag^?: SAUPAN005018000
• symbol: SA_RS10085
• pan gene symbol^?: scn
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS10085 [old locus tag: SA1754 ]
• symbol: SA_RS10085
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2006528..2006878
• length: 351
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (2006528..2006878) NCBI
• BioCyc: SA_RS10085 BioCyc
• MicrobesOnline: see SA1754

⊟Phenotype

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⊟DNA sequence

• 1
  61
  121
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  301
  
  ATGAAAATTAGAAAATCTATACTTGCGGGAACTTTAGCAATCGTTTTAGCATCACCACTA
  GTAACTAATCTAGATAAAAATGAGGCACAAGCTAGCACAAGCTTGCCAACATCGAATGAA
  TATCAAAACGAAAAGTTAGCTAATGAATTAAAATCGTTATTAGATGAACTAAATGTTAAT
  GAATTAGCTACTGGAAGTTTAAACACTTATTATAAGCGAACTATAAAAATTTCAGGTCTA
  AAAGCAATGTATGCTCTTAAGTCAAAAGACTTTAAGAAAATGTCAGAAGCAAAATATCAA
  CTTCAAAAGATTTATAACGAAATTGACGAAGCACTAAAAAGTAAATATTAA
  60
  120
  180
  240
  300
  351
  

⊟Protein

⊟General

• locus tag: SA_RS10085 [old locus tag: SA1754 ]
• symbol: SA_RS10085
• description: hypothetical protein
• length: 116
• theoretical pI: 9.83807
• theoretical MW: 13052
• GRAVY: -0.453448

⊟Function

• TIGRFAM:
  pyrimidine 5'-nucleotidase (TIGR01993; EC 3.1.3.5; HMM-score: 13.3)
  MJ0570-related uncharacterized domain (TIGR00290; HMM-score: 13.1)
  integral membrane protein (TIGR04561; HMM-score: 12.4)
• TheSEED: see SA1754
• PFAM:
  no clan defined CompInhib_SCIN; Staphylococcal complement inhibitor SCIN (PF11546; HMM-score: 164.7)
  and 6 more
  IDEAL; IDEAL domain (PF08858; HMM-score: 17.4)
  EcoR124_C; Type I restriction and modification enzyme - subunit R C terminal (PF12008; HMM-score: 17.3)
  Minor_capsid_3; Minor capsid protein from bacteriophage (PF12691; HMM-score: 16)
  SNARE-fusion (CL0445) Syntaxin_2; Syntaxin-like protein (PF14523; HMM-score: 15.5)
  HUP (CL0039) Diphthami_syn_2; Diphthamide synthase (PF01902; HMM-score: 15)
  no clan defined FapA; Flagellar Assembly Protein A (PF03961; HMM-score: 13.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helices: 0
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 31 aa
  • SP(Sec/SPI): 0.966405
  • TAT(Tat/SPI): 0.009268
  • LIPO(Sec/SPII): 0.009014
  • Cleavage Site: CS pos: 31-32. AQA-ST. Pr: 0.8237
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446624916 NCBI
• RefSeq: WP_000702262 NCBI
• UniProt: see SA1754

⊟Protein sequence

• MKIRKSILAGTLAIVLASPLVTNLDKNEAQASTSLPTSNEYQNEKLANELKSLLDELNVNELATGSLNTYYKRTIKISGLKAMYALKSKDFKKMSEAKYQLQKIYNEIDEALKSKY

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SaeR see SA1754

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications