PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS10515 [old locus tag: SA1832 ]
• pan locus tag^?: SAUPAN005252000
• symbol: SA_RS10515
• pan gene symbol^?: —
• synonym:
• product: excisionase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS10515 [old locus tag: SA1832 ]
• symbol: SA_RS10515
• product: excisionase
• replicon: chromosome
• strand: -
• coordinates: 2069699..2070016
• length: 318
• essential: no DEG

⊟Accession numbers

• Location: NC_002745 (2069699..2070016) NCBI
• BioCyc: SA_RS10515 BioCyc
• MicrobesOnline: see SA1832

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  
  ATGTTCAACATTAATATTGATGAAGATGAAGCACGTGAGTTACTTGAGCAGGCTATCAAT
  GCACGTGTGGACGAATTAGCGAAAGAGAAATATTTTATGACTTACAAAGAGTTGTCTAAC
  TATCTGAATTTAAGTAAGCCTACTATTGAAGAATTACTTATTAATAATGGCATGAAATAT
  TATATGGTCGGATCTACGTATAGATTCAAAAAGTCTGATGTAGATGAATTCATGGAACAG
  CTTACTGCTCATATGAATATCCAGAATAACGACTTTAAACAAGTCAATATCAAAAAGTTA
  TTGGAGGCAAGGCAATGA
  60
  120
  180
  240
  300
  318
  

⊟Protein

⊟General

• locus tag: SA_RS10515 [old locus tag: SA1832 ]
• symbol: SA_RS10515
• description: excisionase
• length: 105
• theoretical pI: 4.7476
• theoretical MW: 12523.2
• GRAVY: -0.658095

⊟Function

• TIGRFAM:
  Unknown function General DNA binding domain, excisionase family (TIGR01764; HMM-score: 70.2)
  and 2 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase PcrA (TIGR01073; EC 3.6.4.12; HMM-score: 14.2)
  ParB/RepB/Spo0J family partition protein (TIGR00180; HMM-score: 12.3)
• TheSEED: see SA1832
• PFAM:
  HTH (CL0123) HTH_17; Helix-turn-helix domain (PF12728; HMM-score: 41.8)
  and 5 more
  Tn916-Xis; Excisionase from transposon Tn916 (PF09035; HMM-score: 16.2)
  HTH_Mga; M protein trans-acting positive regulator (MGA) HTH domain (PF08280; HMM-score: 14.9)
  NUMOD1; NUMOD1 domain (PF07453; HMM-score: 13.9)
  HTH_10; HTH DNA binding domain (PF04967; HMM-score: 13.3)
  HTH_5; Bacterial regulatory protein, arsR family (PF01022; HMM-score: 12.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005708
  • TAT(Tat/SPI): 0.000272
  • LIPO(Sec/SPII): 0.001546
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446404112 NCBI
• RefSeq: WP_000481967 NCBI
• UniProt: see SA1832

⊟Protein sequence

• MFNINIDEDEARELLEQAINARVDELAKEKYFMTYKELSNYLNLSKPTIEELLINNGMKYYMVGSTYRFKKSDVDEFMEQLTAHMNIQNNDFKQVNIKKLLEARQ

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications