PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS12185 [old locus tag: SA2120 ]
• pan locus tag^?: SAUPAN005815000
• symbol: SA_RS12185
• pan gene symbol^?: —
• synonym:
• product: N-acetyl-L,L-diaminopimelate deacetylase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS12185 [old locus tag: SA2120 ]
• symbol: SA_RS12185
• product: N-acetyl-L,L-diaminopimelate deacetylase
• replicon: chromosome
• strand: -
• coordinates: 2383746..2384867
• length: 1122
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (2383746..2384867) NCBI
• BioCyc: SA_RS12185 BioCyc
• MicrobesOnline: see SA2120

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGGGATTAAAAGATCAAGCTGTTACCTGGAGAAGGTATTTTCATCAATTTCCAGAACTA
  TCCGATAAAGAATTTAAGACTACACAAAAAATTAAAGATATTTTAACAGAACATCATATT
  AGAATATTAGACTTACCGCTTATGACTGGGCTGGTTGCTGAAGTGGGACAAGGTCTGAGT
  TGTATAGCTGTTCGGGCTGACATTGATGCTTTACCGATTCAAGAGCTTGTTGAACAGGAT
  TTTAAATCTGAAAATGAAGGTGTTATGCATGCGTGCGGTCATGATATTCACATGGCTAGT
  ATTTTGGCTACGGCTGTAAAATTAAAAGAAATTGAGGGCACTCTTACTGGGCGTGTTAAA
  TTCATTTTTCAACCAGCAGAAGAACTAGGACATGGCGCTTTTAAAATTATTGAAACAGAT
  GCATTAAAAGATGTACAAGCAGTATTGGGTTTTCATAATGACCCATCGCGTTCGGTAGGT
  ACATTTGCAATCAAAACAGGGGCAATTACATCAGCAGTAGATCGCTTTGAGTTTCATATT
  AAAGGCGTGGGTGGTCATGCTGCAAAGCCAGAACAATGCAACGATCCAGTTATTGTGTTG
  GCGCAATTGATTAATAGTATTCAATCCATAGTTAGTAGAAATCTATCTGCGTTTGATGAA
  GCGGTAGTAACAATTGGACAAATATCATGTGGTAATACTTGGAATGTCATTGCTGATCAT
  GCGTATGTACAAGGCACAGTGCGTAGTTTTGATCCTGTGGTACGAAAGCTTGTAGAAACA
  CGATTACAAGATATTGCGGACGGATTGGCTCAAGCATATAATATGAAAATTAATTTAAAT
  TATACACATTTACCTGGCGCTGTGATGAATGATGAGGCATTGACGCATAAAGCAATTGCA
  GTAGCCCAACATGTTGGATATAAGGTGGAAATGATGGAACAGCCGCTTACGATTGGAGAA
  GATTTTTCAGGTTATAGTCAACATTTTCCAAGTGTATTTGCGTTAATTGGTTCTCATAGT
  GAATATGATTTACATCACCCTCAATATAAACCGGATGAAAGGATTCTGGAAAAAGTACCT
  GAGTATTTTGTTGAATTTGTAAAGAGACTATTGCATGAGTAA
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  1122
  

⊟Protein

⊟General

• locus tag: SA_RS12185 [old locus tag: SA2120 ]
• symbol: SA_RS12185
• description: N-acetyl-L,L-diaminopimelate deacetylase
• length: 373
• theoretical pI: 5.99258
• theoretical MW: 41516.1
• GRAVY: -0.0949062

⊟Function

• TIGRFAM:
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides amidohydrolase (TIGR01891; HMM-score: 417.3)
  and 6 more
  Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01246; EC 3.5.1.18; HMM-score: 40.6)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase, ArgE/DapE family (TIGR01910; EC 3.4.-.-; HMM-score: 38.3)
  amidase, hydantoinase/carbamoylase family (TIGR01879; EC 3.5.-.-; HMM-score: 27.9)
  Metabolism Amino acid biosynthesis Glutamate family acetylornithine deacetylase (ArgE) (TIGR01892; EC 3.5.1.16; HMM-score: 25.8)
  Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01900; EC 3.5.1.18; HMM-score: 21.9)
  putative selenium metabolism hydrolase (TIGR03526; HMM-score: 16)
• TheSEED: see SA2120
• PFAM:
  Peptidase_MH (CL0035) Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 100.7)
  and 2 more
  no clan defined M20_dimer; Peptidase dimerisation domain (PF07687; HMM-score: 36.5)
  JAB (CL0366) RadC; RadC-like JAB domain (PF04002; HMM-score: 12.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004805
  • TAT(Tat/SPI): 0.000384
  • LIPO(Sec/SPII): 0.000206
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446445302 NCBI
• RefSeq: WP_000523157 NCBI
• UniProt: see SA2120

⊟Protein sequence

• MGLKDQAVTWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILDLPLMTGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASILATAVKLKEIEGTLTGRVKFIFQPAEELGHGAFKIIETDALKDVQAVLGFHNDPSRSVGTFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAFDEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMKINLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFALIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications