PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS12195 [old locus tag: SA2122 ]
• pan locus tag^?: SAUPAN005818000
• symbol: SA_RS12195
• pan gene symbol^?: hutU
• synonym:
• product: urocanate hydratase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS12195 [old locus tag: SA2122 ]
• symbol: SA_RS12195
• product: urocanate hydratase
• replicon: chromosome
• strand: -
• coordinates: 2386381..2388042
• length: 1662
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (2386381..2388042) NCBI
• BioCyc: SA_RS12195 BioCyc
• MicrobesOnline: see SA2122

⊟Phenotype

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⊟DNA sequence

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  ATGAGAAAAATTCAAGCAAAAAAAGGTTTGAGTATTGAGTGTAAAGGTTGGGAACAAGAA
  GCAGTACTTAGAATGTTATACAACAATTTAGACCCAGAAGTTGCTGAAAGACCAGAAGAT
  TTAGTTGTTTATGGTGGTATTGGAAAAGCAGCACGTAATTGGGAAGCTTTTGAAGCAATA
  GAGAAAACGTTACGAGAATTAGAATCAGATGAGACAATGTTAGTACAATCAGGAAAGCCA
  GTTGCTGTATTTAAAACACATGAAGAAGCGCCACGTGTATTAATTTCAAATTCAGTATTA
  GTGCCTGAATGGGCAAACTGGGATCATTTTAATGAATTAGATAAAAAAGGTTTAATCATG
  TATGGACAGATGACAGCCGGTAGTTGGATATATATTGGTTCTCAAGGTATTGTGCAAGGT
  ACTTATGAGACATTTGCCGAGCTAGGTAATCAACACTTTAATGGAGATTTAGCAGGCACT
  GTTACACTGACAGCAGGTTTAGGTGGTATGGGTGGTGCGCAACCGTTAGCAATTACTATG
  AATCATGGGGTAGCAATTTGCGTGGATGTTGATGAAACACGTGTTGATAAGCGAATTGAT
  ACGAAATACTGTGATGTTAAAACAGCTGATTTAGATGAAGCATTAAAATTAGCAGAAGAG
  GCGAAAGAACGTGGAGAAGGATTATCAATCGGGTTAGTTGGAAATGCTGTAGACATCCAT
  CAAGCGATTCTAGAAAAAGGATTTAAAATTGACATTATTACTGACCAAACAAGTGCACAT
  GATCCGCTAAATGGATATGTGCCACAAGGATACTCTGTAGAAGAAGCGAAAGTATTGCGT
  GAAAAAGATCCGAAAAAATATGTTGAACTGTCACAAGCTTCAATGGCAAAGCATGTTGAA
  TTAATGCTTGAATTCCAAAAACGTGGCGCTGTAGCATTTGATTATGGTAACAATATTCGT
  CAAGTAGCCTTCAATAACGGAGTGAAAAATGCTTTTGACTTCCCAGGCTTTGTACCAGCT
  TACATTAGACCATTATTCTGTGAAGGTAAAGGGCCATTCCGCTTTGCTGCGTTGAGTGGT
  GATCCAAAAGATATCGAGCGTGCGGATGAAGAAATGCGCAAACTTTTCCCAGAAAATGAA
  AAGTTATTAAGATGGCTTGATTTAGCTGAAGAAAAAATTTCATATCAAGGACTACCATCA
  CGTATTGCTTGGTTAGGCTATGGAGAAAGAGCGAAAATGGGCTTAGCTTTAAATCGTCTT
  GTACGTGACGGTGAAATTTCAGCACCAATTGTTATTGGACGAGACCATTTAGATGCTGGT
  TCAGTTGCTAGTCCTAACCGTGAAACAGAAAGTATGAAAGATGGCAGTGACGCGGTTGGT
  GATTGGGCTGTATTGAATGCACTCATTAACACAGCTGCAGGTGGTTCATGGATTTCATTC
  CATCACGGTGGCGGTGTTGGCATGGGATATTCACTTCATGCGGGTATGGTTGTTGTAGCA
  GATGGATCAGAGCGTGCTGAAAGAAGATTGGAACGTGTATTGACGACTGATCCAGGTATG
  GGTGTTGCCCGACATGTTGATGCTGGCTATGACATCGCTATTCAAACAGCTAAAGAAAAA
  GGTATTCATATTCCAATGATTGATAAAGCAGGTGATAAATAA
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⊟Protein

⊟General

• locus tag: SA_RS12195 [old locus tag: SA2122 ]
• symbol: SA_RS12195
• description: urocanate hydratase
• length: 553
• theoretical pI: 5.03205
• theoretical MW: 60632.4
• GRAVY: -0.292586

⊟Function

• reaction:
  EC 4.2.1.49?  ExPASy
  Urocanate hydratase 3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate = urocanate + H₂O
• TIGRFAM:
  Metabolism Energy metabolism Amino acids and amines urocanate hydratase (TIGR01228; EC 4.2.1.49; HMM-score: 969.4)
• TheSEED: see SA2122
• PFAM:
  NADP_Rossmann (CL0063) Urocanase; Urocanase Rossmann-like domain (PF01175; HMM-score: 302.8)
  no clan defined Urocanase_C; Urocanase C-terminal domain (PF17392; HMM-score: 299)
  and 3 more
  Urocanase_N; Urocanase N-terminal domain (PF17391; HMM-score: 203.8)
  TPR (CL0020) TPR_14; Tetratricopeptide repeat (PF13428; HMM-score: 12.3)
  no clan defined Exonuc_VII_S; Exonuclease VII small subunit (PF02609; HMM-score: 11.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: NAD⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00465
  • TAT(Tat/SPI): 0.000226
  • LIPO(Sec/SPII): 0.00071
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447149567 NCBI
• RefSeq: WP_001226823 NCBI
• UniProt: see SA2122

⊟Protein sequence

• MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVYGGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVPEWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGTVTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLAEEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEAKVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDFPGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEKISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETESMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAERRLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA, HutR* see SA2122

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications