PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS12630 [old locus tag: SA2203 ]
• pan locus tag^?: SAUPAN005949000
• symbol: SA_RS12630
• pan gene symbol^?: mdeA
• synonym:
• product: drug:proton antiporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS12630 [old locus tag: SA2203 ]
• symbol: SA_RS12630
• product: drug:proton antiporter
• replicon: chromosome
• strand: -
• coordinates: 2471701..2473104
• length: 1404
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (2471701..2473104) NCBI
• BioCyc: SA_RS12630 BioCyc
• MicrobesOnline: see SA2203

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGCTGATGGGTGGATTTTTTGGATTATTAAATGAAACACTATTAGTGACGGCTTTACCA
  AGTATTATGAAAGATTTTGAAATTTCATATACACAAGTTCAATGGCTGACAACAGCTTTT
  TTATTGACTAATGGGATTGTTATTCCTTTGTCCGCGCTTGTTATACAACGTTATACAACA
  AGACAAGTGTTTTTAGTGGGTATTTCTATCTTTTTCTTAGGTACATTACTCGGCGGCTTG
  AGTCCGCACTTTGCAACATTATTAGTTGCTAGAATTATTCAGGCGTTAGGCGCAGGTATT
  ATGATGCCATTGATGATGACAACGATTTTGGATGTTTTCCAACCGCATGAACGCGGTAAA
  TATATGGGGATATTTGGTTTGGTAATTGGTTTAGCACCAGCTATTGGACCTACTCTTTCA
  GGTTACCTTGTTGAATATTTAAACTGGAGATCGCTTTTCCATGTTGTCGCTCCAATTGCA
  GCTGTTACATTTTTAATTGGATTTAAAACGATAAAAAATGTTGGAACTACAATTAAAGTA
  CCTATTGATTTTATTTCTGTCATTTTTTCTGTACTAGGTTTCGGCGGGTTATTGTATGGA
  ACGAGTTCAATTTCAGAAAAAGGTTTTGATAATCCTATCGTATTAGTATCTATGATTGGA
  GGCGTTGTTTTAGTCGCATTATTTGTATTACGTCAATATCGGCTATCAACACCATTATTA
  AATTTTGCTGTATTTAAAAATAAACAATTTACAGTTGGTATCATTATTATGGGTGTCACA
  ATGGTATCGATGATTGGTTCGGAAACGATTTTACCTATCTTTGTGCAAAATTTATTGCAT
  CGTTCAGCTTTAGATTCTGGATTAACTTTATTACCAGGAGCAATTGTTATGGCATTTATG
  TCGATGACTTCGGGTGCTTTATATGAAAAGTTTGGTCCTAGAAAGCTTGCTTTAGTAGGT
  ATGGCGATTGTTGTTATTACTACGGCTTATTTTGTTGTAATGGATGAACAAACATCAACA
  ATCATGTTGGCAACAGTTTATGCGATTCGAATGGTTGGTATCGCGTTAGGATTAATTCCA
  GTAATGACCCATACGATGAATCAATTAAAGCCAGAAATGAATGCACACGGTTCATCTATG
  ACAAACACAGTACAACAAATTGCTGGTTCTATCGGAACAGCTGCATTAATTACTATTTTA
  TCTCATGCAAGCAAAAACTTTTCTCCAACTATGTCAGACTATAATGGCATGAACAAAATT
  GATATGATGAATCAAATTAAAGTTGATACGATGTTGCATGGTTATCACGCTGGATTTTTA
  TTTGCATTATTGATTACAGTTGTTAGTTTCTTTTGTTCATTTATGTTACAAGGGAAGAAA
  AAAGAAGTTGATTCTCGTCAATAA
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⊟Protein

⊟General

• locus tag: SA_RS12630 [old locus tag: SA2203 ]
• symbol: SA_RS12630
• description: drug:proton antiporter
• length: 467
• theoretical pI: 9.85404
• theoretical MW: 50764.5
• GRAVY: 0.777088

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 337.8)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 337.8)
  and 16 more
  drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 93.6)
  multidrug resistance protein (TIGR00880; HMM-score: 81)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 49.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 48.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 44.9)
  Metabolism Transport and binding proteins Unknown substrate MFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 33.7)
  Metabolism Transport and binding proteins Anions Na(+)-dependent inorganic phosphate cotransporter (TIGR00894; HMM-score: 28)
  oxalate/formate antiporter (TIGR04259; HMM-score: 26.5)
  Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 26.4)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 20.9)
  Metabolism Transport and binding proteins Anions nitrite transporter (TIGR00886; HMM-score: 18)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids monocarboxylate transporter (TIGR00892; HMM-score: 17.7)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 17)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids polyol permease (TIGR00897; HMM-score: 15.8)
  synaptic vesicle protein SV2 (TIGR01299; HMM-score: 7.5)
  sodium-independent organic anion transporter (TIGR00805; HMM-score: 5.2)
• TheSEED: see SA2203
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 180.4)
  and 3 more
  MFS_2; MFS/sugar transport protein (PF13347; HMM-score: 29.9)
  OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 9.2)
  no clan defined MerC; MerC mercury resistance protein (PF03203; HMM-score: 6.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 14
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006867
  • TAT(Tat/SPI): 0.000203
  • LIPO(Sec/SPII): 0.010616
• predicted transmembrane helices (TMHMM): 13

⊟Accession numbers

• GI: see SA2203
• RefSeq: WP_072416042 NCBI
• UniProt: see SA2203

⊟Protein sequence

• MLMGGFFGLLNETLLVTALPSIMKDFEISYTQVQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVARIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYLNWRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISEKGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSMIGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRKLALVGMAIVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSMTNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAGFLFALLITVVSFFCSFMLQGKKKEVDSRQ

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: Zur* see SA2203

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications