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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS12710 [old locus tag: SA2215 ]
  • pan locus tag?: SAUPAN005967000
  • symbol: SA_RS12710
  • pan gene symbol?: bioD
  • synonym:
  • product: dethiobiotin synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS12710 [old locus tag: SA2215 ]
  • symbol: SA_RS12710
  • product: dethiobiotin synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 2486019..2486705
  • length: 687
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (2486019..2486705) NCBI
  • BioCyc: SA_RS12710 BioCyc
  • MicrobesOnline: see SA2215

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAGGATTTTTATTACAAGTACGAATACTGATGTAGGCAAAACCTATGTTACAAAGCAT
    TTATACCATGCTTTGAAAACACGTGGTCATCGTGTTTGTGTTTTTAAACCATTTCAAACT
    GAGGAACGCCAAGACGGGACATTTCCAGATTTAGAAGTATTTAAAAATGAATGTGATTTA
    AGCTATGACATAACGTCACTTTATACTTTTAAGCAACCTGTATCACCACACCTTGCATTT
    AAAATGACAGATCAAATTTTTCTAAATAAGCAGCGTGTATTAGATAAGGTAAAAGTTTTA
    GATAAGGAATTTGATTTTATCTTAATTGAGGGTGCTGGGGGAATTGCCGTACCAATATAT
    GAAGGTACAGATGATTTCTACATGACTAAAGATCTAATCAATGATTGTGCAGATTGTGTC
    ATCAGTGTGTTGCCATCAAAATTAGGTGCTATTAGCGATGCCGTTGTTCACCAAGATTAT
    GTTAATAAGAATGTATCGGCGAGTAATTTTTTAATAATGAATCGCTATACAGACAGCTAT
    ATTGAAAAAGACAATCAAATTACGATTGGAAAATTAACAAATAAAACAGTCTATACATTT
    GAAGAACATGCCACGTATGAAAATTTCTCAGAAGCATTTTTAAAACAATTAATAGGAGTT
    AAAAATGAATTACACACAACAACTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    687

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS12710 [old locus tag: SA2215 ]
  • symbol: SA_RS12710
  • description: dethiobiotin synthase
  • length: 228
  • theoretical pI: 5.89072
  • theoretical MW: 26112.5
  • GRAVY: -0.265351

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.3.3?  ExPASy
    Dethiobiotin synthase ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin dethiobiotin synthase (TIGR00347; EC 6.3.3.3; HMM-score: 103.2)
    and 5 more
    Cellular processes Cellular processes Cell division septum site-determining protein MinD (TIGR01968; HMM-score: 30.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobyric acid synthase CobQ (TIGR00313; EC 6.3.5.10; HMM-score: 20.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdopterin-guanine dinucleotide biosynthesis protein B (TIGR00176; HMM-score: 13.9)
    cell division ATPase MinD (TIGR01969; HMM-score: 13.2)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides cellulose synthase operon protein YhjQ (TIGR03371; HMM-score: 12.1)
  • TheSEED: see SA2215
  • PFAM:
    P-loop_NTPase (CL0023) AAA_26; AAA domain (PF13500; HMM-score: 86.5)
    and 4 more
    CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 25.6)
    DUF1611; Domain of unknown function (DUF1611_C) P-loop domain (PF07755; HMM-score: 21.4)
    MobB; Molybdopterin guanine dinucleotide synthesis protein B (PF03205; HMM-score: 17.8)
    cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 13.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009671
    • TAT(Tat/SPI): 0.000295
    • LIPO(Sec/SPII): 0.001452
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCVFKPFQTEERQDGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMTDQIFLNKQRVLDKVKVLDKEFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAVVHQDYVNKNVSASNFLIMNRYTDSYIEKDNQITIGKLTNKTVYTFEEHATYENFSEAFLKQLIGVKNELHTTT

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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