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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS12730 [old locus tag: SA2217 ]
  • pan locus tag?: SAUPAN005972000
  • symbol: SA_RS12730
  • pan gene symbol?: —
  • synonym:
  • product: ABC transporter ATP-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS12730 [old locus tag: SA2217 ]
  • symbol: SA_RS12730
  • product: ABC transporter ATP-binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2488916..2490679
  • length: 1764
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (2488916..2490679) NCBI
  • BioCyc: SA_RS12730 BioCyc
  • MicrobesOnline: see SA2217

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAACAATCAGACAAATTGGATAAAGATACTCAGCGGATTTGCTTCTGATAGTAAATGG
    AAAATTATGTTATCAATTTTGTTGTCTATCATCAGTGTCTTTTCTGGATTAGTTCCATAT
    TGGGCAGTCTTTAAAATTATTTTAATGATGATTAACAATGCATATACGATTAATTCGATT
    ATGGTTTATATCTTTATTGCTTTAATCACTTATATTTCACAAGTGTGTTGTTTTGGAGCG
    TCAACGATGCTATCACATATTACGGCATATGAAATTTTATCTGAAATTCGTAAAAAATTA
    GCTCAAAAATTAATGCGCCTCCCTTTAGGCGTAGTGGAATCTAAGAAAATAGGTGAATTA
    AAAAATATATTTGTCGATAAGGTTGAAACAATAGAATTACCTTTAGCACATATGATTCCT
    GAAGTTATAGGAAACTTACTTTTGTCAGCTGCTATCTTTTTATACATAATGCTCATTGAT
    TGGCGTATGGCTAGTGCCTTATTAGTAACGATACCAATTTCAATTTTCGCTTTTAAAAAA
    GTTATGTCTGGATTTAATGAGACATATGCTGAACAAATGAAATCAAATAATTATATGAAT
    AGCGCGATTGTAGAGTTTATCGAAGGCATTGAAGTGATTAAAACATTTAATCAATCTCAA
    AGTTCATATAAAAAATATAAAGATGCAGTAGATAATTATAAGATTCACACTTTGAATTGG
    TTTAAAAATACATGGGGGTATATGAATTTAGGAGCTAGTGTATTACCTTCAACATTTTTA
    GGGATTTTACCGGTCGGCATGTATTTGATATCTATCAACCAATTAAACTATGCGGAATTT
    TTCCTATGCATCGTATTATCTTTAGGTGTAGTAGCACCGATTAAGAATTTTACTAATTAT
    GTAAATCATTTAAAGTCTATACAATACGCATTAACTGAAGTGAATCAAATATTAAGTCTA
    GAAGAGTTAGTATTGTCAACAAAATTTAAGAAGCCTCAACATTATGAAATTGCTTTTAAT
    AATGTTGGATTTTCATATAACAAAGATAAGGACGATCTGGTCTTTAAGCATTTATCATTT
    ACAGTACCAGAAAATAATTTCACAGCTATCGTCGGGGCTTCTGGTAGCGGTAAGTCAACC
    ATTGCCAAGCTTATATCACGATACTGGGATGTGACTTCAGGTGAAATTACGATTGGCGGT
    ATAAATATAAAAGATATTGAATCGAAACAACTTAACGATTTAGTTGGTTTTGTTGGACAA
    GATAACTTTTTATTAAATCTTACTTTTAAAGAAAATATTAAACTTGGCAATCCAGAAGCT
    ACGGATGAGGCAGTTGAAAAAGCTGCCAAGTTAGCACAATGTCATGAATTTATTGAAAAG
    TTGCCAGATGGATATGATACGAATGTTGGTACAGTGGGAGATAAATTGTCTGGTGGTGAA
    AAACAACGAGTCACTATTGCAAGAATGATATTAAAAGATGCGCCTATCATTGTATTAGAT
    GAAGCGACTGCTTACGTTGATCCAGACAACGAACAAAAAATTCAAGAAGCATTAAATGTG
    TTGACGCAGGATAAAACATTGATTGTTATTGCACATCGGTTATCTACGATTCAACATGCA
    GATCAAATTATTGTTTTAGGTAAACAACAAATTTTAGAAAAAGGGTCACATCACTTATTG
    CTAAAATTAAACGGTAATTATAAGAAAATGTGGGATACGCACATGCATACAAAAGATTGG
    GGAATCAATACTGGACATAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1764

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS12730 [old locus tag: SA2217 ]
  • symbol: SA_RS12730
  • description: ABC transporter ATP-binding protein
  • length: 587
  • theoretical pI: 7.71696
  • theoretical MW: 66087.6
  • GRAVY: 0.129642

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides lipid A export permease/ATP-binding protein MsbA (TIGR02203; HMM-score: 341.1)
    Metabolism Transport and binding proteins Other lipid A export permease/ATP-binding protein MsbA (TIGR02203; HMM-score: 341.1)
    ABC transporter, permease/ATP-binding protein (TIGR02204; HMM-score: 310)
    thiol reductant ABC exporter, CydD subunit (TIGR02857; HMM-score: 295.6)
    Metabolism Transport and binding proteins Other antigen peptide transporter 2 (TIGR00958; HMM-score: 281.6)
    and 72 more
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR01846; HMM-score: 272.2)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking ABC-type bacteriocin transporter (TIGR01193; HMM-score: 271.4)
    Genetic information processing Protein fate Protein modification and repair ABC-type bacteriocin transporter (TIGR01193; HMM-score: 271.4)
    Metabolism Transport and binding proteins Other ABC-type bacteriocin transporter (TIGR01193; HMM-score: 271.4)
    Cellular processes Cellular processes Pathogenesis type I secretion system ATPase (TIGR03375; HMM-score: 269)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR03375; HMM-score: 269)
    Cellular processes Cellular processes Biosynthesis of natural products NHLM bacteriocin system ABC transporter, peptidase/ATP-binding protein (TIGR03796; HMM-score: 240.8)
    Metabolism Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system ABC transporter, peptidase/ATP-binding protein (TIGR03796; HMM-score: 240.8)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids glucan exporter ATP-binding protein (TIGR01192; EC 3.6.3.42; HMM-score: 232.7)
    thiol reductant ABC exporter, CydC subunit (TIGR02868; HMM-score: 218)
    Cellular processes Cellular processes Biosynthesis of natural products NHLM bacteriocin system ABC transporter, ATP-binding protein (TIGR03797; HMM-score: 212.1)
    Metabolism Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system ABC transporter, ATP-binding protein (TIGR03797; HMM-score: 212.1)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR01842; HMM-score: 188)
    Metabolism Transport and binding proteins Other multi drug resistance-associated protein (MRP) (TIGR00957; HMM-score: 150.4)
    Metabolism Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04520; EC 3.6.3.-; HMM-score: 133.6)
    Cellular processes Cellular processes Toxin production and resistance putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 113.6)
    Metabolism Transport and binding proteins Unknown substrate putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 113.6)
    Metabolism Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04521; EC 3.6.3.-; HMM-score: 112.1)
    Cellular processes Cellular processes Cell division cell division ATP-binding protein FtsE (TIGR02673; HMM-score: 102)
    Metabolism Transport and binding proteins Anions cystic fibrosis transmembrane conductor regulator (CFTR) (TIGR01271; EC 3.6.3.49; HMM-score: 97.2)
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, ATP-binding protein (TIGR00972; EC 3.6.3.27; HMM-score: 96.9)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids ABC transporter, ATP-binding subunit, PQQ-dependent alcohol dehydrogenase system (TIGR03864; HMM-score: 94.8)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking lipoprotein releasing system, ATP-binding protein (TIGR02211; EC 3.6.3.-; HMM-score: 92.5)
    Metabolism Transport and binding proteins Amino acids, peptides and amines glycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 92)
    Metabolism Transport and binding proteins Anions phosphonate ABC transporter, ATP-binding protein (TIGR02315; EC 3.6.3.28; HMM-score: 91.9)
    Metabolism Transport and binding proteins Other thiamine ABC transporter, ATP-binding protein (TIGR01277; EC 3.6.3.-; HMM-score: 85.6)
    Metabolism Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtE (TIGR03410; HMM-score: 85.4)
    ABC exporter ATP-binding subunit, DevA family (TIGR02982; HMM-score: 83.4)
    Metabolism Transport and binding proteins Amino acids, peptides and amines cyclic peptide transporter (TIGR01194; HMM-score: 81.5)
    Metabolism Transport and binding proteins Other cyclic peptide transporter (TIGR01194; HMM-score: 81.5)
    Metabolism Transport and binding proteins Anions molybdate ABC transporter, ATP-binding protein (TIGR02142; EC 3.6.3.29; HMM-score: 75.3)
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel import ATP-binding protein NikE (TIGR02769; EC 3.6.3.24; HMM-score: 74.1)
    gliding motility-associated ABC transporter ATP-binding subunit GldA (TIGR03522; HMM-score: 74)
    Metabolism Transport and binding proteins Anions sulfate ABC transporter, ATP-binding protein (TIGR00968; EC 3.6.3.25; HMM-score: 73.8)
    proposed F420-0 ABC transporter, ATP-binding protein (TIGR03873; HMM-score: 72.9)
    Metabolism Transport and binding proteins Other pigment precursor permease (TIGR00955; HMM-score: 71.4)
    ATP-binding cassette protein, ChvD family (TIGR03719; HMM-score: 70.5)
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel import ATP-binding protein NikD (TIGR02770; HMM-score: 68.7)
    lantibiotic protection ABC transporter, ATP-binding subunit (TIGR03740; HMM-score: 68.5)
    Metabolism Transport and binding proteins Amino acids, peptides and amines putative 2-aminoethylphosphonate ABC transporter, ATP-binding protein (TIGR03265; HMM-score: 68.1)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides LPS export ABC transporter ATP-binding protein (TIGR04406; HMM-score: 67.2)
    Metabolism Transport and binding proteins Other LPS export ABC transporter ATP-binding protein (TIGR04406; HMM-score: 67.2)
    Metabolism Transport and binding proteins Other daunorubicin resistance ABC transporter, ATP-binding protein (TIGR01188; HMM-score: 67)
    Metabolism Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtD (TIGR03411; HMM-score: 66.9)
    phosphonate C-P lyase system protein PhnL (TIGR02324; HMM-score: 66.7)
    D-methionine ABC transporter, ATP-binding protein (TIGR02314; EC 3.6.3.-; HMM-score: 64.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other FeS assembly ATPase SufC (TIGR01978; HMM-score: 64.2)
    Metabolism Transport and binding proteins Cations and iron carrying compounds cobalt ABC transporter, ATP-binding protein (TIGR01166; HMM-score: 64.1)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids peroxysomal long chain fatty acyl transporter (TIGR00954; HMM-score: 62.5)
    Cellular processes Cellular processes Other nodulation ABC transporter NodI (TIGR01288; HMM-score: 62.3)
    Metabolism Transport and binding proteins Other nodulation ABC transporter NodI (TIGR01288; HMM-score: 62.3)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 61.7)
    Metabolism Transport and binding proteins Other heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 61.7)
    Metabolism Transport and binding proteins Anions nitrate ABC transporter, ATP-binding proteins C and D (TIGR01184; HMM-score: 57)
    Metabolism Transport and binding proteins Other nitrate ABC transporter, ATP-binding proteins C and D (TIGR01184; HMM-score: 57)
    2-aminoethylphosphonate ABC transport system, ATP-binding component PhnT (TIGR03258; HMM-score: 55.1)
    Metabolism Transport and binding proteins Amino acids, peptides and amines polyamine ABC transporter, ATP-binding protein (TIGR01187; EC 3.6.3.31; HMM-score: 54.9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines choline ABC transporter, ATP-binding protein (TIGR03415; HMM-score: 54.7)
    Metabolism Energy metabolism Methanogenesis methyl coenzyme M reductase system, component A2 (TIGR03269; HMM-score: 54.4)
    ectoine/hydroxyectoine ABC transporter, ATP-binding protein EhuA (TIGR03005; HMM-score: 53.9)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit A (TIGR00630; EC 3.1.25.-; HMM-score: 44.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-xylose ABC transporter, ATP-binding protein (TIGR02633; EC 3.6.3.17; HMM-score: 43.9)
    Metabolism Transport and binding proteins Other rim ABC transporter (TIGR01257; HMM-score: 43.4)
    Metabolism Transport and binding proteins Other pleiotropic drug resistance family protein (TIGR00956; HMM-score: 41)
    Metabolism Transport and binding proteins Unknown substrate anchored repeat-type ABC transporter, ATP-binding subunit (TIGR03771; HMM-score: 40.1)
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02168; HMM-score: 32.6)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02168; HMM-score: 32.6)
    Metabolism Central intermediary metabolism Phosphorus compounds phosphonate C-P lyase system protein PhnK (TIGR02323; HMM-score: 30.4)
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02169; HMM-score: 26)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02169; HMM-score: 26)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA repair protein RecN (TIGR00634; HMM-score: 12.3)
    Genetic information processing Transcription RNA processing polynucleotide kinase-phosphatase (TIGR04075; HMM-score: 10.9)
  • TheSEED: see SA2217
  • PFAM:
    P-loop_NTPase (CL0023) ABC_tran; ABC transporter (PF00005; HMM-score: 105.6)
    ABC_membrane (CL0241) ABC_membrane; ABC transporter transmembrane region (PF00664; HMM-score: 89)
    and 20 more
    P-loop_NTPase (CL0023) SMC_N; RecF/RecN/SMC N terminal domain (PF02463; HMM-score: 41.7)
    AAA_21; AAA domain, putative AbiEii toxin, Type IV TA system (PF13304; HMM-score: 21.4)
    AAA_23; AAA domain (PF13476; HMM-score: 21.2)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 20)
    AAA_15; AAA ATPase domain (PF13175; HMM-score: 17.8)
    DUF87; Domain of unknown function DUF87 (PF01935; HMM-score: 17.5)
    ABC_ATPase; Predicted ATPase of the ABC class (PF09818; HMM-score: 17.2)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 15.9)
    AAA_18; AAA domain (PF13238; HMM-score: 15.6)
    NB-ARC; NB-ARC domain (PF00931; HMM-score: 15.2)
    AAA_22; AAA domain (PF13401; HMM-score: 15)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 14.9)
    AAA_30; AAA domain (PF13604; HMM-score: 14.9)
    SbcCD_C; Putative exonuclease SbcCD, C subunit (PF13558; HMM-score: 13.3)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 13.2)
    AAA_10; AAA-like domain (PF12846; HMM-score: 11.9)
    no clan defined DUF3987; Protein of unknown function (DUF3987) (PF13148; HMM-score: 11.4)
    NADP_Rossmann (CL0063) Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 11.2)
    P-loop_NTPase (CL0023) AAA_25; AAA domain (PF13481; HMM-score: 11)
    no clan defined DUF4229; Protein of unknown function (DUF4229) (PF14012; HMM-score: 7.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001985
    • TAT(Tat/SPI): 0.000136
    • LIPO(Sec/SPII): 0.029055
  • predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNNQTNWIKILSGFASDSKWKIMLSILLSIISVFSGLVPYWAVFKIILMMINNAYTINSIMVYIFIALITYISQVCCFGASTMLSHITAYEILSEIRKKLAQKLMRLPLGVVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAIFLYIMLIDWRMASALLVTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEVIKTFNQSQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMYLISINQLNYAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQILSLEELVLSTKFKKPQHYEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFTAIVGASGSGKSTIAKLISRYWDVTSGEITIGGINIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEAVEKAAKLAQCHEFIEKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLDEATAYVDPDNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLLLKLNGNYKKMWDTHMHTKDWGINTGHN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:
    SA_RS08505aldehyde dehydrogenase  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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